Use of Radioimmunoassay to Determine the Nature, Quantity and Source of Allergenic Contamination of Sunflower Butter

1983 ◽  
Vol 46 (7) ◽  
pp. 625-628 ◽  
Author(s):  
JOHN W. YUNGINGER ◽  
MARY B. GAUERKE ◽  
RICHARD T. JONES ◽  
MARY JO E. DAHLBERG ◽  
STEVEN J. ACKERMAN

A girl known to be allergic to peanuts experienced a mild allergic reaction after eating sunflower butter, a peanut butter facsimile prepared from sunflower nuts. After learning that the same manufacturing plant also produced peanut butter, we examined several sunflower butter preparations for peanut butter contamination by a solid-phase radioimmunoassay which used naturally-occurring immunoglobulin E (IgE) antibodies from known peanut-sensitive individuals. Peanut butter contamination, ranging from 0.3 to 3.3%, was found in six of eight sunflower butter samples, including both creamy and chunky varieties, and including lots prepared with either peanut oil or palm oil. We concluded that inadequate cleaning of food processing machines following peanut butter production permitted the contamination by peanut butter of subsequent jars of sunflower butter in amounts which could pose risks to peanut-sensitive individuals.

PEDIATRICS ◽  
1990 ◽  
Vol 85 (5) ◽  
pp. 946-959

Purpose of Study The purpose of this paper was to report clinical and laboratory findings from seven cases of fatal anaphylaxis due to foods. Study Population The report was based on the findings from seven patients ranging from 11 to 43 years of age: two female and five male patients. The cases were evaluated over a 16-month period. Methods Serum samples were obtained from six of the seven cases during resuscitation attempts or at autopsy. Food-specific IgE antibodies were measured by solid-phase radioimmunoassay. Findings Elevated levels of IgE antibodies to the incriminated foods were present in the six cases where the serum was available for study. Three were due to peanut and one each were due to pecan, crab, and cod. The seventh case (without serum) was due to peanut. The patients had some features in common: Most were highly atopic; most reactions occurred away from home; all had previously had generalized immediate reactions to the food; and none were on β-adrenergic blocking agents. Several recommendations are made by the authors. Patients and physicians must be made aware of the potential consequences of severe systemic reactions to foods. Patients and physicians must know that the immediate treatment is injectable, aqueous adrenalin not oral antihistamines, nor epinephrine by metered-dose aerosol or in suspension. The patients should have the adrenalin available to them at all times and know how to administer it. Patients receiving concomitant steroid therapy should be assumed to have adrenal suppression and treated appropriately during resuscitation. Ingredients of prepared foods must be made available and patients (and/or their families) must use this information to try to avoid catastrophic results.


1981 ◽  
Vol 64 (5) ◽  
pp. 1077-1082 ◽  
Author(s):  
Ossama El-Nakib ◽  
James J Pestka ◽  
Fun S Chu

Abstract Determinations of aflatoxin B1 in corn, wheat, and peanut butter by an enzyme-linked immunoassay (ELISA) and a solid-phase radioimmunoassay (RIA) were compared. Samples spiked with 2.9-43.2 ppb B1 were subjected to AOAC extraction procedure 26.017 or 26.023. The extracts were concentrated, redissolved in methanol, diluted in phosphate-buffered saline with Tween 20, and directly analyzed for B1 by either ELISA or RIA. At 5.8 ppb or greater, recoveries for B1 in corn, wheat, and peanut butter samples were 80.0,86.6, and 94.8% by ELISA and 61.0, 93.3, and 110.0% by RIA, respectively. Recoveries greater than 120% were obtained for the wheat and peanut butter samples spiked with 2.9 ppb aflatoxin B1 by the RIA method but not by ELISA. Overall results indicated that ELISA gave more consistent data, relatively lower standard deviations, and lower coefficients of variation than did RIA. Analysis of 3 samples naturally contaminated with aflatoxins revealed that the ELISA data were comparable to those obtained by other established chemical methods.


1968 ◽  
Vol 20 (01/02) ◽  
pp. 001-006 ◽  
Author(s):  
K. J Catt ◽  
J Hirsh ◽  
D. J Castelan ◽  
H. D Niall ◽  
G. W Tregear

SummaryThe solid-phase radioimmunoassay method has been applied to the measurement of fibrinogen. The method is extremely sensitive, being able to detect fibrinogen concentrations as low as 10 ng/ml. The immunoreactivity of fibrinogen proteolysis products differs from that of native fibrinogen, early proteolysis products showing enhanced immunoreactivity which decreases progressively with further digestion.


Science ◽  
1967 ◽  
Vol 158 (3808) ◽  
pp. 1570-1572 ◽  
Author(s):  
K. Catt ◽  
G. W. Tregear

1979 ◽  
Vol 29 (3) ◽  
pp. 263-270 ◽  
Author(s):  
S. Romagnani ◽  
G.F. Del Prete ◽  
Grazia M. Giudizi ◽  
F. Almerigogna ◽  
M. Ricci

Author(s):  
Stephen A. Tillson ◽  
Ian H. Thorneycroft ◽  
Guy E. Abraham ◽  
Rex J. Scaramuzzi ◽  
Burton V. Caldwell

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