A Simple Method for the Characteristic Differentiation of Antibiotics by TLC-Bioautography in Graded Concentration of Ammonium Chloride
1988 ◽
Vol 51
(10)
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pp. 786-789
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A simple classification method for 24 known antibiotics by TLC-bioautographic procedure was developed. The approach used was to change the Rf values in seven TLC systems with an ammonium chloride solution in a graded concentration range (0.5, 1, 2, 3, 5, 10 and 20%). The antibiotics were divided into four groups (A to D) showing the characteristic behavior of Rf values corresponding to similarities in chemical structure: β-lactam, aminoglycoside, macrolide and tetracycline antibiotics. TLC-bioautography helps to estimate the character of antibiotics and the characteristic change of Rf values may be very useful for classifying unknown residual antibiotics in animal samples as a routine laboratory test.
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1989 ◽
Vol 32
(1)
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pp. 47-68
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1982 ◽
Vol 15
(1)
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pp. 17-21
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2016 ◽
Vol 26
(6)
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pp. 1688-1695
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1964 ◽
Vol 28
(3)
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pp. 221-227
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2001 ◽
Vol 33
(7-8)
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pp. 1037-1048
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