Phylogenetic Analysis of Nuclear Ribosomal DNA Intergenic Spacer (IGS) I Region of Phellinus linteus

In this study, phylogenetic analysis of Turkish Conringia (Brassicaceae) species was conducted based on nuclear ribosomal DNA (nrITS) and chloroplast DNA (trnL-F) sequences. In addition, the relationships between the sequences of some Brassicaceae family species retrieved from NCBI, and Conringia species were documented. All of the plant specimens were collected at their flowering and vegetation periods from different regions of Turkey, and brought to the laboratory. Total genomic DNA was extracted using the GeneMark kit. In PCR analyses, ITS4 and ITS5A primers were used for the amplification of the nrITS region, while the trnLe and trnLf primers were used for the cpDNA trnL-F region. The DNA sequences obtained were then edited using BioEdit and FinchTV, and analyzed using MEGA 6.0 software. Neighbor joining (NJ) and bootstrap trees were constructed in order to identify the relationships among Conringia taxa. The nrITS sequences ranged between 573 and 672 nucleotides, while the trnL-F sequences ranged between 346 and 764 nucleotides. The divergence values of nrITS sequences differed between 0.177 and 0.00 and divergence values of trnL-F sequences differed between 0.902 and 0.00. NJ tree generated using nrITS and trnL-F sequences consisted of two clades. In trees generated with both the nrITS and trnL-F sequences, C. orientalis, C. grandiflora and C. austriaca appeared within the same group. In addition, according to the phylogenetic analysis results obtained with other Brassicaceae species, it is revealed that the Conringia genus is polyphyletic.

Download Full-text

Euphorbia mbuinzauensis, a new succulent species in Kenya from the Synadenium group in Euphorbia sect. Monadenium (Euphorbiaceae)

PhytoKeys ◽

10.3897/phytokeys.183.70285 ◽

2021 ◽

Vol 183 ◽

pp. 21-35

Author(s):

Neng Wei ◽

Fredrick Munyao Mutie ◽

Geoffrey Mwachala ◽

Olwen M. Grace ◽

Guang-Wan Hu ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Ribosomal Dna ◽

Phylogenetic Inference ◽

Distinct Species ◽

Nuclear Ribosomal Dna ◽

Molecular Phylogenetic Analysis ◽

Molecular Phylogenetic ◽

Smooth Margin ◽

Iucn Criteria

Euphorbia mbuinzauensis, a succulent new species of the Synadenium group in Euphorbiaceae from Makueni County, Kenya, is described and illustrated. Morphologically, it is most similar to E. pseudomollis, but differs mainly by its shrubby habit (up to 4 m), abaxial leaves surfaces with densely stellate hairs, 2–4-forked cymes, smaller bracts (ca. 2.5 × 3.0 mm), smaller cyathia (6 mm wide), crimson glands without narrow smooth margin, smaller fruits (ca. 8 × 7 mm) and ovoid seeds (ca. 1.8 × 2.2 mm). Furthermore, we performed a molecular phylogenetic analysis of the Synadenium group in Euphorbia sect. Monadenium, based on complete nuclear ribosomal DNA (nrDNA) datasets. This phylogenetic inference also supports it to be a distinct species. The new species is assessed as Endangered using the IUCN criteria.

Download Full-text