Antheia to access Ginkgo’s enzyme screening and design technology

In light of a growing research interest in the innovation potential that lies at the intersection of design, technology, and science, this paper offers a literature review of design initiatives centered on scientific discovery and invention. The focus of this paper is on evidence of design capabilities in the academic research environment. The results are structured along the Four Orders of Design, with examples of design-in-science initiatives ranging from (1) the design of scientific figures and (2) laboratory devices using new technology to (3) interactions in design workshops for scientists and (4) interdisciplinary design labs. While design capabilities have appeared in all four orders of design, there are barriers and cultural constraints that have to be taken into account for working at or researching these creative intersections. Modes of design integration and potentially necessary adaptations of design practice are therefore also highlighted.

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An Approach to Assessing the Environmental Impact Tradeoffs from Design, Technology, and Flight Procedures in Airport Area Operations

9th AIAA Aviation Technology, Integration, and Operations Conference (ATIO) ◽

10.2514/6.2009-7048 ◽

2009 ◽

Author(s):

Paul Brett ◽

Michelle Kirby ◽

Dimitri Mavris

Keyword(s):

Environmental Impact ◽

Design Technology

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Development of 96 Multiple Injection-GC-MS Technique and Its Application in Protein Engineering of Natural and Non-Natural Enzymatic Reactions

10.26434/chemrxiv.10314239 ◽

2019 ◽

Author(s):

Anja Knorrscheidt ◽

Pascal Püllmann ◽

Eugen Schell ◽

Dominik Homann ◽

Erik Freier ◽

...

Keyword(s):

Microtiter Plate ◽

Axial Ligand ◽

Cell System ◽

Enzymatic Reactions ◽

The Novel ◽

Internal Standards ◽

E Coli ◽

Enzyme Screening ◽

Plate Analysis ◽

Microtiter Plate Analysis

Directed evolution requires the screening of enzyme libraries in biological matrices. Available assays are mostly substrate or enzyme specific. Chromatographic techniques like LC and GC overcome this limitation, but require long analysis times. The herein developed multiple injections in a single experimental run (MISER) using GC coupled to MS allows the injection of samples every 33 s resulting in 96-well microtiter plate analysis within 50 min. This technique is implementable in any GC-MS system with autosampling. Since the GC-MS is far less prone to ion suppression than LCMS, no chromatographic separation is required. This allows the utilisation of an internal standards and the detection of main and side-product. To prove the feasibility of the system in enzyme screening, two libraries were assessed: i) YfeX library in an E. coli whole cell system for the carbene-transfer reaction on indole revealing the novel axial ligand tryptophan, ii) a library of 616 chimeras of fungal unspecific peroxygenase (UPO) in S. cerevisiae supernatant for hydroxylation of tetralin resulting in novel constructs. The data quality and representation are automatically assessed by a new R-script.

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