scholarly journals Zoonotic Surveilance (Brucellosis) in Enrekang District South Sulawesi Province

2021 ◽  
Vol 1 (2) ◽  
pp. 122-127
Author(s):  
Nur Alim Ahzan ◽  
Muhasirah Muhasirah ◽  
Nurhayati Nurhayati ◽  
Rosmiaty Rosmiaty

Brucellosis merupakan penyakit zoonosis yang disebabkan oleh bakteri genus brucella, brucellosis dapat menular dari hewan ke manusia.  Tujuan penelitian untuk mengetahui risiko penularan brucellosis pada peternak, pekerja peternakan dan petugas kesehatan hewan di Kabupaten Enrekang Propinsi Sulawesi Selatan. Metode Sampel darah diambil dengan menggunakan tenaga analis dengan tabung vakutainer, kemudian dilakukan centrifuge dan dibiarkan pada temperatur kamar sampai terbentuk serum. Serum kemudian di tetesi dengan RBT pada slide, hasil RBT positif kemudian diuji konfirmasi dengan dengan PCR-Konvensional  di Laboratorium Balai Teknik Kesehatan Lingkungan dan Pengendalian Penyakit Kelas I Makassar. Hasil penelitian berdasarkan uji RBT (Rose Bengal Test) didapatkan 3 sampel positif   dengan kategori positif +++ dan 1 sampel dengan kategori positif ++. dan setelah dilakukan uji konfirmasi terhadap sampel dengan metode PCR Konvensional didapatkan 2 sampel positif. Kesimpulan: Terdapat kasus penularan brucellosis pada peternak dan petugas kesehatan hewan di Kabupaten Enrekang.

2011 ◽  
Vol 5 (4) ◽  
pp. e950 ◽  
Author(s):  
Ramón Díaz ◽  
Aurora Casanova ◽  
Javier Ariza ◽  
Ignacio Moriyón

1933 ◽  
Vol 3 (1) ◽  
pp. 55-60 ◽  
Author(s):  
W. Parker Stowe ◽  
G. D. Delprat ◽  
Alanson Weeks

2010 ◽  
Vol 31 (2) ◽  
pp. 120-130 ◽  
Author(s):  
Donghee Cho ◽  
Hyangmi Nam ◽  
Jongwan Kim ◽  
Eunjeong Heo ◽  
Yunsang Cho ◽  
...  

Author(s):  
S.I.B. Cadmus ◽  
H.K. Adesokan ◽  
J. Stack

In this study, milk and blood samples collected simultaneously from 532 trade cows to be slaughtered at Bodija abattoir, Ibadan (southwestern, Nigeria) were examined for antibodies to Brucella using the milk ring test (MRT) and the rose bengal test (RBT). Overall, 18.61%of the milk samples were positive according to the MRT, while 9.77 % of the serum samples were positive according to the RBT. The difference was highly significant (Chi-square value 16.33; P<0.05); only 32 (6.02 %) of the samples were positive for both tests. The Red Bororo breed of cattle and the White Fulani had the highest positive rates, namely 20.93 % and 11.69%for the MRT and RBT respectively.No conclusion can be drawn about sensitivity because we do not know the true status of the animals tested. It is, however, obvious that although the MRT and RBT are 1st-line screening tests for brucellosis in cows in some countries, their lack of specificity is of concern. Therefore, the requirement for other confirmatory tests that are more specific should be considered for control and eradication of the disease, especially in Nigeria.


Author(s):  
A. Zorić ◽  
I. Krdžić
Keyword(s):  

Bruceloza je zarazno oboljenje domaćih, divljih životinja i ljudi, rašireno u celomsvetu. Dijagnostika bruceloze putem izolacije uzročnika je veoma teška, dugotrajnai predstavlja rizik od infekcije. Iz tih razloga, serološke metode predstavljaju metodeizbora.Cilj rada je bila uporedna analiza specifi čnosti i osetljivosti testova za dijagnostikubruceloze koji se rutinski koriste na teritoriji Republike Srbije.Ukupno je ispitano 120 uzoraka krvnog seruma goveda na prisustvo specifi čnihantitela protiv Brucella spp. Svi serumi su prvo ispitani primenom Rose Bengal testa.Uzorci koji su pokazali pozitivnu reakciju su zatim ispitivani primenom iELISA i cELISAtesta. Izračunavanje osetljivosti i specifi čnosti seroloških testova je sprovedenona osnovu dobijenih rezultata.Analizom osetljivosti i specifi čnosti zaključeno je da su obe metode (Rose Bengaltest, iELISA test) visoko osetljive (100%, 92%) sa razlikom od 8% u korist iELISA testa,koji se pokazao i kao daleko specifi čnija metoda (91%) u odnosu na Rose Bengaltest (69%).Uporednom analizom rezultata dobijenih u ispitivanju osetljivosti i specifi čnostiRose Bengal testa i indirektne imunoenzimske metode (iELISA) u otkrivanju specifičnih antitela protiv Brucella spp. u krvnim serumima goveda, utvđena je većaosetljivost i specifi čnost metode iELISA u odnosu na Rose Bengal test.


2012 ◽  
Vol 35 (2) ◽  
pp. 133-137
Author(s):  
Md. Siddiqur Rahman ◽  
Amitavo Chakrabartty ◽  
Md. Taohidul Islam ◽  
Roma Rani Sarker ◽  
M.E. Alam ◽  
...  
Keyword(s):  

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