scholarly journals High Frequency Somatic Embryogenic Callus Induction and Plant Regeneration from Various Indica Rice Genotypes

2006 ◽  
Vol 33 (4) ◽  
pp. 257-262
Author(s):  
Md. Enamul Hoque ◽  
John W. Mansfield
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
High Frequency ◽  
Embryogenic Callus ◽  
Indica Rice ◽  
Rice Genotypes ◽  
Embryogenic Callus Induction ◽  
Somatic Embryogenic

scholarly journals Embryogenic Callus Induction and Regeneration of Elite Bangladeshi Indica Rice Cultivars

1970 ◽  
Vol 17 (1) ◽  
pp. 65-70 ◽  
Author(s):  
ME Hoque ◽  
MS Ali ◽  
NH Karim
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Indica Rice ◽  
Rice Cultivars ◽  
Embryogenic Calli ◽  
Rice Callus ◽  
Embryogenic Callus Induction ◽  
Embryogenic Callus Formation

Significant variations were observed among six elite Bangladeshi Indica rice cultivars tested in relation to total callus induction frequency (p = 0.017), embryogenic callus formation frequency (p = 0.001) and subsequent plant regeneration responses (p = 0.005). In all the cases, embryogenic callus formation frequency was much more less than the total callus (embryogenic + non-embryonegic) formation frequency. The embryogenic calli derived from mature seed embryos produced green plants, successfully established in soil and produced fertile seeds.Key words: Indica rice, Callus induction, Plant regeneration, Genotypic variationsDOI = 10.3329/ptcb.v17i1.1122Plant Tissue Cult. & Biotech. 17(1): 65-70, 2007 (June)

scholarly journals High frequency plant regeneration from embryogenic callus of a popular indica rice (Oryza sativa L.)

2009 ◽  
Vol 15 (4) ◽  
pp. 371-375 ◽  
Author(s):  
Alagarsamy Karthikeyan ◽  
Shunmugiah Thevar Karutha Pandian ◽  
Manikandan Ramesh
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
High Frequency ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice

Effect of 2,4-D on Embryogenic Callus Induction of Malaysian indica Rice (Oryza sativa L.) Cultivars MR123 and MR127

2013 ◽  
Vol 64 (2) ◽  
Author(s):  
Fauziah Illyas Ahmad ◽  
Nur Shafiqoh Johan ◽  
Alina Wagiran
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Staining Method ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Simple Protocol ◽  
Basal Media ◽  
Embryogenic Callus Induction ◽  
Mature Seeds

The aim of this study is to study the effect of various concentrations of 2,4-D on embryogenic callus induction of indica rice MR123 cultivar and MR127 cultivar using mature seeds. Optimal media for induction of callus of both cultivars was MS basal media supplemented with 30g/L sucrose, 500mg/L glutamine and 500mg/L proline supplemented with 2.5 mg/L 2,4-D. The highest percentage of callus induction was 70% and 76% for MR123 and MR127 respectively. Both cultivars produced an embryogenic callus from scutellum after a week in culture with white-yellowish in color. The viability tested using Evans blue show callus obtained was embryogenic. This simple protocol using staining method could be used for screening embryogenic callus before further experiments can be conducted.

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