scholarly journals When morphogenetic proteins encounter special extracellular matrix and cell-cell connections at the interface of the renal stem/progenitor cell niche

2015 ◽  
Vol 48 (1) ◽  
pp. 1 ◽  
Author(s):  
Will W. Minuth ◽  
Lucia Denk
Keyword(s):  
Extracellular Matrix ◽  
Progenitor Cell ◽  
Cell Niche ◽  
Cell Cell

Peculiarities of the extracellular matrix in the interstitium of the renal stem/progenitor cell niche

2011 ◽  
Vol 136 (3) ◽  
pp. 321-334 ◽  
Author(s):  
Will W. Minuth ◽  
Lucia Denk ◽  
Christian Miess ◽  
Anne Glashauser
Keyword(s):  
Extracellular Matrix ◽  
Progenitor Cell ◽  
Cell Niche

scholarly journals Enhanced Piezoelectric Fibered Extracellular Matrix to Promote Cardiomyocyte Maturation and Tissue Formation: A 3D Computational Model

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 135
Author(s):  
Pau Urdeitx ◽  
Mohamed H. Doweidar
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
Computational Models ◽  
Cell Junctions ◽  
Tissue Formation ◽  
Cardiac Muscle Cells ◽  
Cell Processes ◽  
Electrical Stimuli ◽  
Cell Cell

Mechanical and electrical stimuli play a key role in tissue formation, guiding cell processes such as cell migration, differentiation, maturation, and apoptosis. Monitoring and controlling these stimuli on in vitro experiments is not straightforward due to the coupling of these different stimuli. In addition, active and reciprocal cell–cell and cell–extracellular matrix interactions are essential to be considered during formation of complex tissue such as myocardial tissue. In this sense, computational models can offer new perspectives and key information on the cell microenvironment. Thus, we present a new computational 3D model, based on the Finite Element Method, where a complex extracellular matrix with piezoelectric properties interacts with cardiac muscle cells during the first steps of tissue formation. This model includes collective behavior and cell processes such as cell migration, maturation, differentiation, proliferation, and apoptosis. The model has employed to study the initial stages of in vitro cardiac aggregate formation, considering cell–cell junctions, under different extracellular matrix configurations. Three different cases have been purposed to evaluate cell behavior in fibered, mechanically stimulated fibered, and mechanically stimulated piezoelectric fibered extra-cellular matrix. In this last case, the cells are guided by the coupling of mechanical and electrical stimuli. Accordingly, the obtained results show the formation of more elongated groups and enhancement in cell proliferation.

scholarly journals Cellular dynamics of EMT: lessons from live in vivo imaging of embryonic development

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Jeffrey D. Amack
Keyword(s):  
Extracellular Matrix ◽  
Embryonic Development ◽  
In Vivo Imaging ◽  
Cytoskeletal Proteins ◽  
Cell Junctions ◽  
Model Organisms ◽  
Cellular Dynamics ◽  
Mesenchymal Transition ◽  
Cell Cell

AbstractEpithelial-mesenchymal transition (EMT) refers to a process in which epithelial cells lose apical-basal polarity and loosen cell–cell junctions to take on mesenchymal cell morphologies and invasive properties that facilitate migration through extracellular matrix. EMT—and the reverse mesenchymal-epithelial transition (MET)—are evolutionarily conserved processes that are used throughout embryonic development to drive tissue morphogenesis. During adult life, EMT is activated to close wounds after injury, but also can be used by cancers to promote metastasis. EMT is controlled by several mechanisms that depend on context. In response to cell–cell signaling and/or interactions with the local environment, cells undergoing EMT make rapid changes in kinase and adaptor proteins, adhesion and extracellular matrix molecules, and gene expression. Many of these changes modulate localization, activity, or expression of cytoskeletal proteins that mediate cell shape changes and cell motility. Since cellular changes during EMT are highly dynamic and context-dependent, it is ideal to analyze this process in situ in living organisms. Embryonic development of model organisms is amenable to live time-lapse microscopy, which provides an opportunity to watch EMT as it happens. Here, with a focus on functions of the actin cytoskeleton, I review recent examples of how live in vivo imaging of embryonic development has led to new insights into mechanisms of EMT. At the same time, I highlight specific developmental processes in model embryos—gastrulation in fly and mouse embryos, and neural crest cell development in zebrafish and frog embryos—that provide in vivo platforms for visualizing cellular dynamics during EMT. In addition, I introduce Kupffer’s vesicle in the zebrafish embryo as a new model system to investigate EMT and MET. I discuss how these systems have provided insights into the dynamics of adherens junction remodeling, planar cell polarity signaling, cadherin functions, and cytoskeletal organization during EMT, which are not only important for understanding development, but also cancer progression. These findings shed light on mechanisms of actin cytoskeletal dynamics during EMT, and feature live in vivo imaging strategies that can be exploited in future work to identify new mechanisms of EMT and MET.

scholarly journals Hydrophilic cell-derived extracellular matrix as a niche to promote adhesion and differentiation of neural progenitor cells

RSC Advances ◽  
2017 ◽  
Vol 7 (72) ◽  
pp. 45587-45594 ◽  
Author(s):  
Lingyan Yang ◽  
Ziyun Jiang ◽  
Linhong Zhou ◽  
Keli Zhao ◽  
Xun Ma ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Basal Forebrain ◽  
Progenitor Cell ◽  
Neural Progenitor Cells ◽  
Cholinergic Neurons ◽  
Neural Progenitor Cell ◽  
Neural Progenitor ◽  
Basal Forebrain Cholinergic Neurons ◽  
Adhesion Performance ◽  
Excellent Adhesion

Cell-derived extracellular matrix exhibits excellent adhesion performance for neural progenitor cell anchoring and residency, resulting in promoted proliferation of the stem cells to basal forebrain cholinergic neurons.

scholarly journals Extracellular matrix and the neural stem cell niche

2011 ◽  
Vol 71 (11) ◽  
pp. 1006-1017 ◽  
Author(s):  
Ilias Kazanis ◽  
Charles ffrench-Constant
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Niche ◽  
Neural Stem Cell Niche ◽  
Cell Niche

scholarly journals Insights into the local pathogenesis induced by fish toxins: Role of natterins and nattectin in the disruption of cell–cell and cell–extracellular matrix interactions and modulation of cell migration

Toxicon ◽  
2011 ◽  
Vol 58 (6-7) ◽  
pp. 509-517 ◽  
Author(s):  
Evilin Naname Komegae ◽  
Anderson Daniel Ramos ◽  
Ana Karina Oliveira ◽  
Solange Maria de Toledo Serrano ◽  
Mônica Lopes-Ferreira ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
Matrix Interactions ◽  
Extracellular Matrix Interactions ◽  
Cell Cell
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