scholarly journals ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF STAPHYLOCOCCUS AUREUS STRAINS IN ISLAMABAD, PAKISTAN

2021 ◽  
Vol 71 (3) ◽  
pp. 1056-60
Author(s):  
Ambreen Gul ◽  
Yasir Rasheed ◽  
Kaleem Imdad ◽  
Raheela Yasmin ◽  
Aneela Jamil ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Minimal Inhibitory Concentration ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Media Analysis ◽  
Aureus Strain ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Methicillin Resistant ◽  
Group 1

Objective: To investigate the prevalence of S. aureus in hospitalized patients of Islamabad. Study Design: Cross-sectional study. Study Duration: Pakistan Institute of Medical Science, Applied Microbiology and Biotechnology Lab, COMSATS Institute of Information Technology, Islamabad, from Sep 2017 to Sep 2018. Methodology: A total of 500 samples were collected. The isolates were divided into four study groups according to their source of origin i.e. group 1 (dermal group), group 2 (nasal group), group 3 (blood group) and group 4 (urine group). Gram staining, catalase test and DNA se media analysis were done for validation of S. aureus. Disc diffusion test (for antibiotic susceptibility), Oxacillin disc test (to differentiate between methicillin-resistant Staphylococcus aureus and methicillin-susceptible staphylococcus aureus) and minimal inhibitory concentration (for susceptibility to vancomycin), were performed. Results: Degree of the prevalence of staphylococcus aureus was 21%, 17%, 9% and 8% in group 1, 2, 3 & 4 respectively. The overall prevalence of staphylococcus aureus was 19.5% in all isolates. The disc diffusion test showed the descending resistance pattern of isolates i.e. 100, 94, 94, 76, 58, 55, 47, 43, 40 and 37% for penicillin, ciprofloxacin, Kanamycin, erythromycin, tetracycline, oxazolidinone, sulfamethoxazole, doxycycline, clindamycin, and cipoxin respectively. Minimal inhibitory concentration found only one sample resistant at 2ug/l concentration of Vancomycin. Moreover, Oxacillin disc test showed 52% methicillin-susceptibleStaphylococcus aureus while 48.2% methicillin-resistant staphylococcus aureus among all isolates. Conclusion: There is an increase in the frequency of methicillin-resistant staphylococcus aureus. Single vancomycin resistant staphylococcus aureus strain was also isolated.

scholarly journals Validity of Cefoxitin Disc Diffusion Test for the Detection of Methicillin-resistant Staphylococcus Aureus

2018 ◽  
Vol 6 (1) ◽  
pp. 26-29 ◽  
Author(s):  
Hafiza Sultana ◽  
Jogendra Nath Sarker ◽  
Md. Abdullah Yusuf ◽  
Md. Tofael Hossain Bhuiyan ◽  
Md. Mostaqimur Rahman ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Methicillin Resistant

scholarly journals Evaluation of MRSA Chrome agar for the detection of methicillin resistant Staphylococcus aureus

2014 ◽  
Vol 7 (1) ◽  
pp. 1-4
Author(s):  
Durdana Chowdhury ◽  
Sanya Tahmina Jhora ◽  
Tarek Mahbub Khan ◽  
Sadia Afroz
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meca Gene ◽  
Clinical Samples ◽  
Clinical Microbiology Laboratory ◽  
Blue Color ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Gold Standard Method ◽  
Methicillin Resistant

The aim of this study was to evaluate the efficacy of MRSA Chrome agar to detect methicillin resistant Staphylococcus aureus (MRSA) and compare it with 1?g oxacillin disc diffusion tests and detection of mecA gene by PCR. A total 116 Staphylococcus aureus (S. aureus), isolated from various clinical samples, were obtained from three tertiary care hospitals of Dhaka city. S. aureus was identified by colony characters, Gram stain and standard biochemical procedures. MRSA was detected by susceptibility to 1?g oxacillin disc, growth of denim blue color colonies of S. aureus on the Brilliance MRSA Chrome agar at 24 and 48 hours of incubation. PCR was performed for amplification of mecA gene as a gold standard method. Out of 116 isolated S. aureus, 33 (28.44%) were MRSA by oxacillin disc diffusion test where mecA gene was detected in 28 strains. On MRSA Chrome agar, 29 (25.0%) S. aureus produced denim blue colonies at 24 hours, of which 28 isolates possessed mecA gene. At 48 hours incubation, an additional 4 isolates yielded denim blue colonies from which mecA gene could not be identified. All the strains of S. aureus that produced denim blue colonies at 24 and 48 hours were resistant to oxacillin. The sensitivity, specificity and accuracy of oxacillin disc diffusion test were 100%, 94.31% and 95.68% and Chrome agar at 24 hours were 100%, 98.86% and 99.13% respectively. Thus MRSA Chrome agar could be good choice in clinical microbiology laboratory for rapid and accurate identification of MRSA. DOI: http://dx.doi.org/10.3329/imcj.v7i1.17697 Ibrahim Med. Coll. J. 2013; 7(1): 1-4

scholarly journals Prevalence of MecC Gene Among Methicillin Resistant Staphylococcus aureus isolated from Patients in Ain-Shams University Hospital

2020 ◽  
Vol 14 (4) ◽  
pp. 2807-2813
Author(s):  
Heba Raafat Shebl ◽  
Wafaa Khalil Zaki ◽  
Ashraf Nabil Saleh ◽  
Shimaa Ahmed Abdel Salam
Keyword(s):  
Staphylococcus Aureus ◽  
Minimal Inhibitory Concentration ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Inhibitory Concentration ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
University Hospital ◽  
University Hospitals ◽  
Methicillin Resistant ◽  
The Uk

Methicillin-Resistant Staphylococcus aureus (MRSA) is an important cause of healthcare associated infections globally. New mecA homologue (mecC), was first reported in the UK and Denmark. The mecC mediated MRSA is resistant only to Β-lactams antibiotics and is sensitive to other antibiotics. Detecting the prevalence of mecC MRSA provides more options in treatment of MRSA infections. The aim of this study was to prevalence of mecC gene in clinical isolates of MRSA in Ain-Shams university hospitals & to correlate Minimal Inhibitory concentration (MIC) of Oxacillin with the mecC gene expression in MRSA isolates. Fifty MRSA isolates were collected from different intensive care units (ICUs) of Ain-Shams university hospital from April-December 2018. Methicillin resistance was detected by Cefoxitin disc, and antimicrobial susceptibility testing was done for all isolates and its results were interpreted according to Clinical & Laboratory Standards Institute (CLSI) guidelines 2018. Minimal Inhibitory Concentration of Oxacillin was detected using Oxacillin E-test and the results were interpreted according to the manufacturer’s instructions, then Polymerase Chain Reaction was done to detect mecA and mecC genes among MRSA isolates. Fifty isolates were identified as MRSA by Cefoxitin disc out of 163 samples. Twelve isolates were sensitive to Oxacillin while 38 isolates were resistant to Oxacillin. All isolates were positive to mecA gene while only 3 isolates were positive to both mecA and mecC genes. MecC is a new emerging gene responsible for methicillin resistance in staphylococci and was detected in 6 % of the isolates in this study.

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