Rapid detection of clarithromycin-resistant
            Helicobacter pylori
            in patients with dyspepsia by fluorescent in situ hybridization (FISH) compared with the E-test

This study reports the use of the fluorescent in situ hybridization (FISH) with Sal3 probe for Salmonella detection in swine carcasses inner surface (swab); and in the correspondent samples of ileum, ileocolic, and mandibular lymph nodes; and tonsils, after dilution (1:10) in buffered peptone water and a pre-enrichment step (37(0)C, 18h). In order to evaluate the efficiency of FISH, 235 naturally contaminated samples were simultaneously tested by the cultural method (ISO 6579) and by the Vitek Immuno Diagnostic Assay System (VIDAS®) - Salmonella (SLM) system. The cultural method identified 39 positive samples. From these, VIDAS®- SLM only detected 23. FISH identified 115 positive samples. This difference was highly significant (P<0.001). From positive samples, 32 were also confirmed by the cultural method. The results indicate FISH as a promising tool for rapid Salmonella detection in samples of pork and swine carcasses.

Download Full-text

Detection of clarithromycin-resistant Helicobacter pylori in frozen gastric biopsies from pediatric patients by a commercially available fluorescent in situ hybridization

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2007.06.020 ◽

2007 ◽

Vol 59 (4) ◽

pp. 421-423 ◽

Cited By ~ 11

Author(s):

Alba E. Vega ◽

Teresa Alarcón ◽

Diego Domingo ◽

Manuel López-Brea

Keyword(s):

Helicobacter Pylori ◽

In Situ Hybridization ◽

Fluorescent In Situ Hybridization ◽

Pediatric Patients ◽

Gastric Biopsies

Download Full-text

Molecular detection of Helicobacter pylori in a large Mediterranean river, by direct viable count fluorescent in situ hybridization (DVC-FISH)

Journal of Water and Health ◽

10.2166/wh.2014.171 ◽

2014 ◽

Vol 12 (4) ◽

pp. 868-873 ◽

Cited By ~ 9

Author(s):

Ilias Tirodimos ◽

Mattheos Bobos ◽

Evangelos Kazakos ◽

Anna-Bettina Haidich ◽

Theodore Dardavessis ◽

...

Keyword(s):

Helicobacter Pylori ◽

In Situ Hybridization ◽

Fluorescent In Situ Hybridization ◽

Potable Water ◽

Molecular Detection ◽

Viable Count ◽

Potential Health ◽

Viable Cells ◽

H Pylori

Although the precise route and mode of transmission of Helicobacter pylori are still unclear, molecular methods have been applied for the detection of H. pylori in environmental samples. In this study, we used the direct viable count fluorescent in situ hybridization (DVC-FISH) method to detect viable cells of H. pylori in the River Aliakmon, Greece. This is the longest river in Greece, and provides potable water in metropolitan areas. H. pylori showed positive detection for 23 out of 48 water samples (47.9%), while no seasonal variation was found and no correlation was observed between the presence of H. pylori and indicators of fecal contamination. Our findings strengthen the evidence that H. pylori is waterborne while its presence adds to the potential health hazards of the River Aliakmon.

Download Full-text