Sequencing Of The Luciferase Gene Of Photinus Pyralis

2015 ◽  
Author(s):  
Mihris Ibnu Saleem ◽  
Aravind S. P. ◽  
Rohini S. ◽  
Harikrishnan Madayath ◽  
Baji K. ◽  
...  
Keyword(s):  
Luciferase Gene ◽  
Photinus Pyralis

scholarly journals Novel Cyanobacterial Biosensor for Detection of Herbicides

2002 ◽  
Vol 68 (10) ◽  
pp. 5026-5033 ◽  
Author(s):  
C. Y. Shao ◽  
C. J. Howe ◽  
A. J. R. Porter ◽  
L. A. Glover
Keyword(s):  
Optical Density ◽  
Environmental Samples ◽  
Environmental Pollutants ◽  
Rapid Screening ◽  
Environmental Damage ◽  
Luciferase Gene ◽  
Cyanobacterial Strain ◽  
Photinus Pyralis ◽  
Freshwater Cyanobacterium ◽  
Successful Expression

ABSTRACT The aim of this work was to generate a cyanobacterial biosensor that could be used to detect herbicides and other environmental pollutants. A representative freshwater cyanobacterium, Synechocystis sp. strain PCC6803, was chromosomally marked with the luciferase gene luc (from the firefly Photinus pyralis) to create a novel bioluminescent cyanobacterial strain. Successful expression of the luc gene during growth of Synechocystis sp. strain PCC6803 cultures was characterized by measuring optical density and bioluminescence. Bioluminescence was optimized with regard to uptake of the luciferase substrate, luciferin, and the physiology of the cyanobacterium. Bioassays demonstrated that a novel luminescent cyanobacterial biosensor has been developed which responded to a range of compounds including different herbicide types and other toxins. This biosensor is expected to provide new opportunities for the rapid screening of environmental samples or for the investigation of potential environmental damage.

Development of a Suite of Luciferase Gene Probes for the Screening and Detection of Marine Bioluminescent Systems and Organisms

2007 ◽  
Author(s):  
M. D. Iglesia-Rodriguez ◽  
Oscar M. Schofield ◽  
Scott M. Glenn ◽  
Mark Moline
Keyword(s):  
Luciferase Gene ◽  
Gene Probes

scholarly journals Genomic Structure of the Luciferase Gene from the Bioluminescent Beetle,Nyctophilacf.Caucasica

2006 ◽  
Vol 6 (37) ◽  
pp. 1-8 ◽  
Author(s):  
John C. Day ◽  
Mohammad J. Chaichi ◽  
Iraj Najafil ◽  
Andrew S. Whiteley
Keyword(s):  
Genomic Structure ◽  
Luciferase Gene

Expression of Luciferase Gene Under Control of the puf Promoter from Rhodobacter sphaeroides

1999 ◽  
pp. 337-345
Author(s):  
Lyudmila Vasilyeva ◽  
Masato Miyake ◽  
Chikashi Nakamura ◽  
Eiji Nakada ◽  
Anatoly Tsygankov ◽  
...  
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Luciferase Gene

scholarly journals Efficient mobilization of E1-deleted adenovirus type 5 vectors by wild-type adenoviruses of other serotypes

2002 ◽  
Vol 83 (6) ◽  
pp. 1311-1314 ◽  
Author(s):  
Hendrik J. Rademaker ◽  
Mohamed A. Abou El Hassan ◽  
Gijs A. Versteeg ◽  
Martijn J. W. E. Rabelink ◽  
Rob C. Hoeben
Keyword(s):  
Hepg2 Cells ◽  
Adenovirus Type ◽  
Firefly Luciferase ◽  
Luciferase Gene ◽  
Wild Type ◽  
Genome Packaging ◽  
Firefly Luciferase Gene ◽  
Adenovirus Vectors ◽  
Initiation Of Dna Replication ◽  
Adenovirus Type 5

Mobilization of replication-deficient adenovirus vectors can lead to spread and shedding of the vector. Here we show that in cultured HepG2 cells wild-type (wt) adenoviruses of subgroup A (Ad12), B (Ad7, 11 and 16), C (Ad1, 2 and 5) and E (Ad4) can efficiently mobilize Ad5CMVluc, a ΔE1ΔE3-Ad5 vector carrying the firefly luciferase gene as reporter. In addition, we show that Ad5CMVluc can be propagated on Ad12E1-transformed human embryonic retinoblasts. This provides evidence that expression of the E1 region of Ad12 is sufficient for mobilizing ΔE1-Ad5-derived vectors. Thus, in therapeutic applications of replication-defective Ad vectors any active Ad infection is of potential concern, independent of the serotype involved. To prevent vector mobilization by wt Ads, new vectors should be developed in which essential functions such as the initiation of DNA replication and genome packaging are restricted.

scholarly journals Firefly genomes illuminate parallel origins of bioluminescence in beetles

2017 ◽  
Author(s):  
Timothy R. Fallon ◽  
Sarah E. Lower ◽  
Ching-Ho Chang ◽  
Manabu Bessho-Uehara ◽  
Gavin J. Martin ◽  
...  
Keyword(s):  
Firefly Luciferase ◽  
Chemical Defenses ◽  
Photinus Pyralis ◽  
Click Beetles ◽  
Origin And Evolution ◽  
The North ◽  
Click Beetle ◽  
Light Organs ◽  
The Caribbean ◽  
Shed Light

AbstractFireflies and their fascinating luminous courtships have inspired centuries of scientific study. Today firefly luciferase is widely used in biotechnology, but the evolutionary origin of their bioluminescence remains unclear. To shed light on this long-standing question, we sequenced the genomes of two firefly species that diverged over 100 million-years-ago: the North AmericanPhotinus pyralisand JapaneseAquatica lateralis.We also sequenced the genome of a related click-beetle, the CaribbeanIgnelater luminosus,with bioluminescent biochemistry near-identical to fireflies, but anatomically unique light organs, suggesting the intriguing but contentious hypothesis of parallel gains of bioluminescence. Our analyses support two independent gains of bioluminescence between fireflies and click-beetles, and provide new insights into the genes, chemical defenses, and symbionts that evolved alongside their luminous lifestyle.One Sentence Summary:Comparative analyses of the first linkage-group-resolution genomes of fireflies and related bioluminescent beetles address long-standing questions of the origin and evolution of bioluminescence and its associated traits.

Nucleocytoplasmic transport of luciferase gene mRNA requires CRM1/Exportin1 and RanGTPase

2009 ◽  
Vol 42 (2) ◽  
pp. 70-81 ◽  
Author(s):  
Tominori Kimura ◽  
Iwao Hashimoto ◽  
Masao Nishikawa ◽  
Hisao Yamada
Keyword(s):  
Nucleocytoplasmic Transport ◽  
Luciferase Gene ◽  
Gene Mrna
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