scholarly journals Effects of polymorphisms at <i>LEP</i>, <i>CAST</i>, <i>CAPN1</i>, <i>GHR</i>, <i>FABP4</i> and <i>DGAT1</i> genes on fattening performance and carcass traits in Simmental bulls

2017 ◽  
Vol 60 (2) ◽  
pp. 61-70 ◽  
Author(s):  
Sena Ardicli ◽  
Deniz Dincel ◽  
Hale Samli ◽  
Faruk Balci
Keyword(s):  
Average Daily Gain ◽  
Carcass Traits ◽  
Meat Production ◽  
The Novel ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Single Nucleotide ◽  
Fattening Performance ◽  
Pcr Rflp ◽  
Fattening Period

Abstract. The aim of this study was to investigate the effects of single nucleotide polymorphisms (SNPs) at six candidate genes (LEP, CAST, CAPN1, GHR, FABP4 and DGAT1) on fattening performance and carcass traits of Simmental bulls in Turkey. The analysis covered a total of 81 Simmental bulls grown on a private farm that were randomly selected for their fattening period for use in this study. Genotyping was performed using the PCR-RFLP method. The S20T polymorphism at the CAST gene and the G316A polymorphism at the CAPN1 gene were associated with variation in final weight, fattening period, weight gain and average daily gain (P < 0.05). In addition, LEP A80V had a significant effect on hot and chilled carcass weight and dressing percentage (P < 0.05). There was no association between GHR S555G, FABP4 V110M and DGAT1 K232A markers with the traits analysed. These results suggested that focusing on the novel effects of LEP, CAST and CAPN1 gene polymorphisms on meat production traits might be useful for marker-assisted selection in Simmental cattle.

scholarly journals Single Nucleotide Polymorphisms of Porcine lncMGPF Regulate Meat Production Traits by Affecting RNA Stability

2021 ◽  
Vol 9 ◽  
Author(s):  
Wei Lv ◽  
Shiyu Zhao ◽  
Yunqing Hou ◽  
Qian Tong ◽  
Yaxin Peng ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Myogenic Differentiation ◽  
Muscle Growth ◽  
Backfat Thickness ◽  
Meat Production ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Muscle Area ◽  
Single Nucleotide ◽  
Pig Breeds

lncMGPF is a novel positive regulator of myogenic differentiation, muscle growth and regeneration in mouse, pig, and human. But whether natural mutations within lncMGPF gene regulate animal meat production traits is unclear. In this study, ten single nucleotide polymorphisms (SNPs) of pig lncMGPF (plncMGPF) gene were identified among commercial pig breeds and Chinese local pig breeds. These SNPs are highly linked and constructed into multiple haplotypes, and haplotype ATTCATGTTC (H1) mainly exists in commercial pig breeds while haplotype GCCTGCACCT (H3) is more frequent in Chinese local pig breeds. Association analysis indicated that all SNPs are significantly associated with the backfat thickness and loin muscle area (P &lt; 0.05), respectively, and homologous H1 individuals have higher loin muscle area and lower backfat thickness than H3 pigs. Bioinformatics and functional analysis showed that haplotype H1 has a longer half-life and more stable RNA secondary structure than haplotype H3. plncMGPF haplotype H1 has stronger effects on pig primary myogenic progenitor cells differentiation and muscle growth than haplotype H3. Further experiments showed that two SNPs (rs81403974 and rs325492834) function together to confer plncMGPF stability and function. Our observation suggested that the SNPs in lncMGPF can change the RNA stabilities and lncMGPF function, thereby affecting the porcine meat production traits.

scholarly journals Association of novel polymorphisms in the bovine myocyte enhancer factor 2D (MEF2D) gene with carcass traits of Polish Holstein-Friesian cattle

2013 ◽  
Vol 58 (No. 6) ◽  
pp. 262-269 ◽  
Author(s):  
E. Juszczuk-Kubiak ◽  
K. Wicińska ◽  
J. Oprządek
Keyword(s):  
Carcass Traits ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Myocyte Enhancer Factor ◽  
Holstein Friesian ◽  
Pcr Rflp ◽  
Combined Genotypes ◽  
Strong Candidate ◽  
Friesian Cattle ◽  
Enhancer Factor

In the present study the polymorphism of the myocyte enhancer factor 2D (MEF2D) gene was analyzed as a genetic marker candidate for carcass traits in Polish Holstein-Friesian cattle. Four novel single nucleotide polymorphisms (SNPs): HQ692911:g.93C&gt;T in exon 8, JX088659:g.69C&gt;T in intron 8, JX0692914:g.47C&gt;T in intron 9 as well as JX088658:g.100G&gt;A in the 3&rsquo;flanking region were identified by DNA sequencing. By applying the PCR-RFLP method, the SNPs (g.93C&gt;T/HaeIII and g.47C&gt;T/Tsp45I) were genotyped in 401 Polish Holstein-Friesian bulls and their association with carcass traits was analyzed. Statistical analysis showed that the g.93C&gt;T SNP was significantly associated with weight of lean (WLVC) and fat in valuable cuts (WFVC). Animals with the TT genotype had significantly higher WLVC (P &lt; 0.05) and lower WFVC (P &lt; 0.05) than those with the CC and CT genotypes. No significant relationship with carcass traits was found for the g.47C&gt;T SNP (P &gt; 0.05) in intron 9. Moreover, we found a significant effect of the combined genotypes onWFVC (P &lt; 0.01), percent of lean (PLVC) (P &lt; 0.05), and fat (PFVC) in valuable cuts (P &lt; 0.01). This suggested that MEF2D is a strong candidate gene that affects carcass traits in cattle.&nbsp;

scholarly journals The novel SNPs of the <i>IGFBP3</i> gene and their associations with litter size and weight traits in goat (Brief report)

2007 ◽  
Vol 50 (2) ◽  
pp. 223-224 ◽  
Author(s):  
X. Y. Lan ◽  
C. Y. Pan ◽  
H. Chen ◽  
M. Zhao ◽  
J. Y. Li ◽  
...  
Keyword(s):  
Growth Factors ◽  
Litter Size ◽  
The Novel ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Coding Region ◽  
Single Nucleotide ◽  
Growth Development ◽  
Igfbp 3 ◽  
Insulin Like Growth Factors

Abstract. The insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene responsible for the multiple effects of insulin-like growth factors (IGFs) playing a key role in mammalian growth, development and reproduction (BALE et al., 1992; HASTIE et al., 2004). Single nucleotide polymorphisms (SNPs) have been described in the bovine IGFBP3 gene which was associated with production traits, as well as in the buffalo (MACIULLA et al., 1997; PADMA et al., 2004). No polymorphism was detected in the sheep IGFBP3 gene (KUMAR et al., 2006). In present experiment most of the coding region and part of introns of IGFBP3 were scanned for SNPs in predominant goat breeds of China. Associations of SNPs of IGFBP3 with litter size and weight traits were analyzed.

scholarly journals Tetra-primer ARMS-PCR identified a missense mutation of the bovine <i>NRIP1</i> gene associated with growth traits

2015 ◽  
Vol 58 (1) ◽  
pp. 165-169
Author(s):  
D. Liu ◽  
Z. Wang ◽  
W. Ma ◽  
Y. Gao ◽  
A. Li ◽  
...  
Keyword(s):  
Growth Traits ◽  
Average Daily Gain ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Interacting Protein ◽  
Association Analyses ◽  
Arms Pcr ◽  
Pcr Rflp ◽  
Cheap Method ◽  
Pcr Method

Abstract. Nuclear receptor-interacting protein 1 (NRIP1) specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors to inhibit transcription. Previous work has demonstrated this protein to be a key regulator in modulating transcriptional activity of many transcription factors, some of which are closely related to development and growth. In this study, we have successfully genotyped two newly identified bovine NRIP1 single-nucleotide polymorphisms (SNPs) (c.605A > G and c.1301G > A) using the T-ARMS-PCR method and validated the accuracy by means of PCR-RFLP assay using 1809 individuals of 9 different cattle breeds. The association analyses results indicated that c.605A > G locus was significantly associated with body weight and average daily gain in Nanyang cattle at 18 months (P < 0.05). Thus it can be inferred that T-ARMS-PCR is a rapid, reliable, and cheap method for SNP genotyping and that c.605A > G polymorphism in bovine NRIP1 is associated with growth traits. These findings will be of benefit for the application of DNA markers related to growth traits in marker-assisted selection (MAS), and will improve the promotion of beef cattle.

scholarly journals Identification of novel single nucleotide polymorphisms in the PRKAG3 gene of Pakistani river buffalo

2019 ◽  
Vol 22 (3) ◽  
pp. 353-358
Author(s):  
W. A. Khan ◽  
T. Hussain ◽  
M. E. Babar ◽  
A. Nadeem ◽  
A. R. Awan ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Catalytic Subunit ◽  
Meat Production ◽  
River Buffalo ◽  
The Novel ◽  
Nucleotide Polymorphisms ◽  
Subunit Gene ◽  
Single Nucleotide ◽  
First Report ◽  
Relationship Of

Pakistani river buffalo is classified in five breeds out of which Kundi buffalo has been least documented. This study is designed to find single nucleotide polymorphisms (SNPs) in exonic and in-tronic regions of PRKAG3 (AMP-activated, Gamma3 non-catalytic subunit) gene in the Kundi buf-falo. The PRKAG3 gene of 95 animals each from Kundi and Nili-Ravi were sequenced for identifica-tion of novel SNPs. Comparing with the Nili Ravi breed of buffalo PRKAG3 gene, six SNP sites were identified in the Kundi buffalo. The novel SNPs found in this work can function as a genomic indicator for genetic-phenotypic relationship of PRKAG3 gene with milk and meat production in buffalo. This is the first report of SNPs in PRKAG3 gene of Kundi Buffalo.

scholarly journals The Investigation of Associations between TP53 rs1042522, BBC3 rs2032809, CCND1 rs9344, EGFR rs2227983 Polymorphisms and Breast Cancer Phenotype and Prognosis

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1419
Author(s):  
Justina Bekampytė ◽  
Agnė Bartnykaitė ◽  
Aistė Savukaitytė ◽  
Rasa Ugenskienė ◽  
Erika Korobeinikova ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Regression Analysis ◽  
Cox Regression ◽  
Cancer Prognosis ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Cox Regression Analysis ◽  
Pcr Rflp ◽  
Oncological Diseases ◽  
Cancer Phenotype

Breast cancer is one of the most common oncological diseases among women worldwide. Cell cycle and apoptosis—related genes TP53, BBC3, CCND1 and EGFR play an important role in the pathogenesis of breast cancer. However, the roles of single nucleotide polymorphisms (SNPs) in these genes have not been fully defined. Therefore, this study aimed to analyze the association between TP53 rs1042522, BBC3 rs2032809, CCND1 rs9344 and EGFR rs2227983 polymorphisms and breast cancer phenotype and prognosis. For the purpose of the analysis, 171 Lithuanian women were enrolled. Genomic DNA was extracted from peripheral blood; PCR-RFLP was used for SNPs analysis. The results showed that BBC3 rs2032809 was associated with age at the time of diagnosis, disease progression, metastasis and death. CCND1 rs9344 was associated with tumor size, however an association resulted in loss of significance after Bonferroni correction. In survival analysis, significant associations were observed between BBC3 rs2032809 and OS, PFS and MFS. EGFR rs2227983 also showed some associations with OS and PFS (univariate Cox regression analysis). However, the results were in loss of significance (multivariate Cox regression analysis). In conclusion, BBC3 rs2032809 polymorphism was associated with breast cancer phenotype and prognosis. Therefore, it could be applied as potential markers for breast cancer prognosis.

scholarly journals Relationship of the bovine IGF1, TG, DGAT1 and MYF5 genes to meat colour, tenderness and cooking loss

2018 ◽  
Vol 69 (3) ◽  
pp. 1077 ◽  
Author(s):  
S. ARDICLI ◽  
H. SAMLI ◽  
D. DINCEL ◽  
B. EKIZ ◽  
H. YALCINTAN ◽  
...  
Keyword(s):  
Muscle Metabolism ◽  
Bovine Insulin ◽  
Cooking Loss ◽  
Farm Animals ◽  
Meat Production ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Colour Parameters ◽  
Meat Colour ◽  
Meat Samples

Bovine insulin-like growth factor 1 (IGF1), thyroglobulin (TG), diacylglycerol-O-acyltransferase 1 (DGAT1) and myogenic factor 5 (MYF5) genes play an important role in the physiology of lipid and muscle metabolism and are therefore considered as candidate genes for meat production traits in farm animals. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in IGF1, TG, DGAT1 and MYF5 genes and to evaluate whether these polymorphisms affected meat colour, tenderness and cooking loss in Holstein cattle. Initially, the SNPs were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. Meat samples (N= 50) derived from M. longissimus thoracis et lumborum (LTL) were used in the current study. Significant differences in variations of meat colour parameters were observed at 24 hours post-mortem. IGF1 was associated with colour parameters of a* and chroma values. In addition, effects of TG were statistically significant on L* and a* values, while, effects of MYF5 were significant on a* value. There was no association of the tested SNPs with meat pH, tenderness and cooking loss. The results presented here may give the valuable information for improving meat colour in cattle.

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