IMAGE ANALYSIS COMBINED FLUORESCENCE MICROSCOPY - Examples of ImageJ Software Application in Yeast Studies

2019 ◽  
Vol 65 (3-4) ◽  
pp. 218-222
Author(s):  
Miloš Gejdoš ◽  
Tomáš Gergeľ ◽  
Tomáš Bucha ◽  
Zuzana Vyhnáliková

Abstract Wood assessment optimization should be the top priority of the forestry subjects that are fundamentally dependent on the income from its sale. The aim of this paper is to analyse the beech, oak and ash tree logs that were categorized into quality classes according to the size of one of the qualitative characters related to the surface area (false heartwood, rot). The classical methodology used in forestry was compared with the application of ImageJ software. In total, thirty logs were analysed. The characters of false heartwood and rot were chosen and evaluated according to their size on the log end. There were no other characters that obstructed the categorization into quality classes. The ImageJ software application led to improved assessment (transfer to a higher quality class) in 56% of the logs. The volume of the evaluated assortments was 18.43 m3. The total difference in the value of the assortments with the ImageJ software application reached + €70.44 (+ 4.7%). The analysis therefore confirmed that in case of a considerable irregularity in a qualitative character (when the surface area of the character significantly differs from the circumscribed circular surface), the standard STN EN 1309–3 methodology systematically overvalues the surface area of this character. That affects the assessment potential of the specific log.


2021 ◽  
pp. 106907
Author(s):  
Lindomar Sousa Brito ◽  
Ana Karina da Silva Cavalcante ◽  
Alexandra Soares Rodrigues ◽  
Priscila Assis Ferraz ◽  
Rodrigo Freitas Bittencourt ◽  
...  

Crop Science ◽  
2002 ◽  
Vol 42 (6) ◽  
pp. 2201-2206 ◽  
Author(s):  
Agustin E. Fonseca ◽  
Mark E. Westgate ◽  
Robert T. Doyle

BioEssays ◽  
2012 ◽  
Vol 34 (5) ◽  
pp. 427-436 ◽  
Author(s):  
Sven van Teeffelen ◽  
Joshua W. Shaevitz ◽  
Zemer Gitai

2017 ◽  
Vol 372 (1720) ◽  
pp. 20150512 ◽  
Author(s):  
Alexandre C. Dufour ◽  
Anneliene H. Jonker ◽  
Jean-Christophe Olivo-Marin

In recent years developmental biology has greatly benefited from the latest advances in fluorescence microscopy techniques. Consequently, quantitative and automated analysis of this data is becoming a vital first step in the quest for novel insights into the various aspects of development. Here we present an introductory overview of the various image analysis methods proposed for developmental biology images, with particular attention to openly available software packages. These tools, as well as others to come, are rapidly paving the way towards standardized and reproducible bioimaging studies at the whole-tissue level. Reflecting on these achievements, we discuss the remaining challenges and the future endeavours lying ahead in the post–image analysis era. This article is part of the themed issue ‘Systems morphodynamics: understanding the development of tissue hardware’.


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