Peroxidative degradation of proteins and lipids in undifferentiated connective tissue dysplasia in children, Ukraine

The aim of the study was to assess the state of lipid and protein peroxidation in children with undifferentiated connective tissue dysplasia (UCTD). In this study, 63 children (33 children with UCTD and 30 children without UCTD were recruited and the indicators of lipid and protein peroxidation were measured. The enzymatic, colorimetric method was used to measure the level of total cholesterol (TC). The phospholipids were analyzed by liquid chromatography/mass spectrometry using electrospray ionization. Lipid peroxidation was studied by assessing the change of index and the end product of lipid peroxidation, malondialdehyde (MDA), using spectrophotometric method. Protein peroxidation - by the content of carbonylated protein based on 2,4 dinitrophenyl-hydrazones derivatization. The activity of the antioxidant defense system enzymes was assessed by measuring catalase (CAT) and superoxide dismutase (SOD). Our study revealed a significant increase of PPO products in the venous blood plasma and LPO products in erythrocytes in children with UCTD. Furthermore, an imbalance of the antioxidant defense system was observed in both blood plasma and erythrocytes membrane.

Download Full-text

Heritability of Some Important Parameters of the Antioxidant Defense System Like Glucose-6-Phosphate Dehydrogenase, Catalase, Glutathione Peroxidase and Lipid Peroxidation in Red Blood Cells by Twin Study

International Journal of Human Genetics ◽

10.1080/09723757.2001.11885764 ◽

2001 ◽

Vol 1 (3) ◽

pp. 1-4 ◽

Cited By ~ 2

Author(s):

Shila Chakraborty ◽

A.B. Das Chaudhuri

Keyword(s):

Lipid Peroxidation ◽

Glutathione Peroxidase ◽

Red Blood Cells ◽

Twin Study ◽

Blood Cells ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Defense System ◽

Glucose 6 Phosphate Dehydrogenase

Download Full-text

Dynamics of Lipid Peroxidation—Antioxidant Defense System during Alloxan Diabetes in Rats

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-013-2071-y ◽

2013 ◽

Vol 155 (1) ◽

pp. 26-29 ◽

Cited By ~ 3

Author(s):

I. A. Volchegorskii ◽

L. M. Rassokhina ◽

I. Yu. Miroshnichenko

Keyword(s):

Lipid Peroxidation ◽

Alloxan Diabetes ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Defense System

Download Full-text

Influence of ellagic acid on antioxidant defense system and lipid peroxidation in mice

Biochemical Pharmacology ◽

10.1016/0006-2952(91)90457-g ◽

1991 ◽

Vol 42 (7) ◽

pp. 1441-1445 ◽

Cited By ~ 41

Author(s):

Sabhiya Majid ◽

Krishan L. Khanduja ◽

Rajinder K. Gandhi ◽

Sanjay Kapur ◽

Rati R. Sharma

Keyword(s):

Lipid Peroxidation ◽

Ellagic Acid ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Defense System

Download Full-text

LIPID PEROXIDATION AND ANTIOXIDANT DEFENSE SYSTEM IN MENOPAUSAL WOMEN OF DIFFERENT ETHNIC GROUPS

Human Ecology ◽

10.33396/1728-0869-2019-6-30-38 ◽

2019 ◽

pp. 30-38

Author(s):

N. V. Semenova ◽

I. M. Madaeva ◽

M. A. Darenskaya ◽

L. I. Kolesnikova

Keyword(s):

Lipid Peroxidation ◽

Ethnic Groups ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Defense System ◽

Menopausal Women

Download Full-text

On the Opportunities of Using the Indices of Lipid Peroxidation – Antioxidant Defense System in the Diagnostics of Varicose Veins of Small Pelvis in Women

Acta biomedica scientifica ◽

10.29413/abs.2020-5.1.2 ◽

2020 ◽

Vol 5 (1) ◽

pp. 14-20

Author(s):

L. I. Kolesnikova ◽

A. A. Semendyaev ◽

D. A. Stupin ◽

M. A. Darenskaya ◽

L. A. Grebenkina ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Defense ◽

Varicose Veins ◽

Antioxidant Defense System ◽

Pathological Process ◽

Moderate Degree ◽

Defense System ◽

Mild Degree ◽

Severe Degree ◽

Degree Of Severity

Background: The prevalence of varicose veins of small pelvic veins in women of reproductive age varies widely – from 5.4 to 80 %, due to the low specificity and low sensitivity of clinical diagnostic techniques, and the absence of biomarkers that can be used to identify the formation and progression of varicose veins of small pelvic veins in women.Aims: To identify changes in the levels of parameters of the system “lipid peroxidation – antioxidant protection” with the development of varicose veins of the pelvis in women, as well as the possibility of their diagnostic use.Materials and methods: We examined 200 women with clinical signs of varicose veins of small pelvis. Control group included 30 women without any pathology of venous system. Treatment group included 137 women with varicose veins of small pelvis: with mild degree of severity – 39 women, with moderate degree of severity – 65 women, with severe degree of severity – 33 women. We performed comparative analysis of lipid peroxidation – antioxidant defense system in women with and without varicose veins of small pelvis taking into account the severity degrees of pathological process.Results: We obtained the most informative indices for lipid peroxidation – antioxidant defense processes in blood of women with varicose veins of small pelvis: concentration of diene conjugate (DC) for the mild degree of severity of pathological process, lipid hydroperoxide (LHP), DC and malonic dialdehyde (MDA) – for the moderate and severe degree of severity, and also changes in the activity of antioxidant enzymes with increase of the severity of the disease. Assessment of the level of catalase (Cat) and glutathione peroxidase (GP) showed their highest informative value at the mild degree of severity. Concentrations of glutathione reductase (GR), glutathione S-transferase (GST) and reduced glutathione (GSH) are more statistically significant markers for severe forms of pathological process.Conclusions: Our research showed the relevance and advisability of the studying the levels of lipid peroxidation – antioxidant defense indices in women with varicose veins of small pelvis. We registered the highest diagnostic value of DC, Cat and GP in the beginning of the disease and of LHP, DC, MDA, GR, GST and GSH – at severe forms of the pathological process. We obtained reference values for DC, MDA, Cat, SOD and GP concentration that can be considered as the predictors of the development of varicose veins of small pelvis.

Download Full-text