UV Spectrophotometric Method Development and Validation of Lignocaine Hydrochloride in Bulk and Semisolid Dosage Form

2021 ◽  
pp. 5280-5282
Author(s):  
Suyash Ingle ◽  
Varsha Tegeli ◽  
Avinash Birajdar ◽  
Vinod Matole ◽  
Sagar Adlinge ◽  
...  
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Spectroscopic Method ◽  
Uv Spectrum ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Development And Validation ◽  
The Given ◽  
Regression Correlation

Objective: A new, simple, sensitive, precise and reproducible UV spectroscopic method was developed for the estimation of Lignocaine Hydrochloride in bulk and Semisolid Formulation. Methods: The UV spectrum of Lignocaine Hydrochloride in RO water showed λ max at 228.8nm. Beer’s law is valid in the concentration range of 20-100µg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 20-100µg/ml with regression equation y = 0.0097x + 0.023 and regression correlation coefficient r2= 0.9992. Moreover, the method was found to be highly sensitive with LOD (3.55μg/ml) and LOQ (10.75μg/ml). Conclusion: Depending on results the given method can be successfully applied for assay of Lignocaine Hydrochloride in Semisolid formulation.

UV Spectrophotometric Method Development and Validation of Carbimazole in Bulk and Tablet Dosage form

2021 ◽  
pp. 163-166
Author(s):  
Zainab A. Bagalkote ◽  
Ganesh Gajeli
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Uv Spectrum ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Uv Visible ◽  
Development And Validation ◽  
Tablet Dosage ◽  
The Given

Objective: A new, simple, sensitive, precise, reproducible UV visible spectrophotometric method was developed for the estimation of Carbimazole in Tablet dosage form. Methods: The UV spectrum of Carbimazole in methanol and distilled water (30:70) showed λ max at 289.6nm. Beer’s law is valid in the concentration range of 10-50µg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 10-50µg/ml with the regression equation y = 0.0232x + 0.0466, and regression coefficient i.e, r2= 0.9992 moreover, the method was found to be highly sensitive with LOD (1.818µg/ml) and LOQ (5.509µg/ml). Conclusion: From the results it can be concluded that the given method can be successfully applied for assay of Carbimazole in Tablet dosage form.

Development and Validation of UV Spectroscopy Method for the Estimation of Dolutegravir in Bulk and Pharmaceutical Dosage Form

2021 ◽  
pp. 188-190
Author(s):  
Vaishnavi Dulange ◽  
G.B. Gajeli
Keyword(s):  
Dosage Form ◽  
Uv Spectroscopy ◽  
Spectroscopic Method ◽  
Water Mixture ◽  
Uv Spectrum ◽  
Pharmaceutical Dosage Form ◽  
Highly Sensitive ◽  
Development And Validation ◽  
The Given ◽  
Regression Correlation

UV spectroscopic method was developed for the estimation of Dolutegravir in bulk and Formulation.The UV spectrum of Dolutegravir in methanol and water mixture showed λ max at 254nm. Beer’s law is valid in the concentration range of 10-50µg/ml. This method was validated for linearity, accuracy, precision, LOD and LOQ. The method has demonstrated excellent linearity over the range of 10-50µg/ml with regression equation y = 0.030x + 0.008 and regression correlation coefficient r2= 0.998. Moreover, the method was found to be highly sensitive with LOD (2.056μg/ml) and LOQ (6.230μg/ml). Depending on results the given method can be successfully applied for assay of Dolutegravir in formulation.

scholarly journals UV VISIBLE SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF DASATINIB IN BULK AND SOLID DOSAGE FORM

2020 ◽  
pp. 90-93
Author(s):  
SAILI MADUR ◽  
VINOD MATOLE ◽  
MALLINATH KALSHETTI
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Uv Spectrum ◽  
Solid Dosage ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Uv Visible ◽  
Development And Validation ◽  
Tablet Dosage

Objective: A new, simple, sensitive, precise, reproducible UV visible spectrophotometric method was developed for the determination of Dasatinib in Tablet dosage form with­­­ methanol. Methods: The method is based on the formation of a colorlesscomplex. The UV spectrum of Dasatinib in methanol showed maximum wavelength at 248 nm. Beer’s law is valid in the concentration range of 7-35µg/ml. this method was validated for linearity, accuracy, precision, assay, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 7-35µg/ml with the regression equation y = 0.0332x+0.0633 and regression coefficient i.e. r2= 0.9994moreover, the method was found to be highly sensitive with LOD(1.08µg/ml) and LOQ(3.29µg/ml). Conclusion: Based on the results the proposed method can be successfully applied for the assay of Dasatinib in various tablet dosage forms.

UV Spectrophotometric Method Development and Validation of Darunavir in bulk and Solid Dosage Form

2021 ◽  
pp. 3262-3264
Author(s):  
Varsha Tegeli ◽  
Avinash Birajdar ◽  
Vinod Matole
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Uv Spectrum ◽  
Solid Dosage ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Tablet Dosage

Objective: A new, simple, sensitive, precise, reproducible UV visible spectrophotometric method was developed for the determination of Darunavir in Tablet dosage form with¬¬¬ 0.1N HCl. Method: The method is based on the formation of a colorless complex. The UV spectrum of Darunavir in 0.1N HCl showed maximum wavelength at 298nm. Beer’s law is valid in the concentration range of 10-60µg/ml. this method was validated for linearity, accuracy, precision, assay, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 10-60µg/ml with the regression equation y=0.0113x+0.0098, and regression coefficient i.e. r2=0.9992 moreover, the method was found to be highly sensitive with LOD (1.85µg/ml) and LOQ (5.62µg/ml). Conclusion: Based on the results the proposed method can be successfully applied for the assay of Darunavir in various tablet dosage forms.

scholarly journals VISIBLE SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF IMATINIB IN BULK AND FORMULATION

2020 ◽  
pp. 63-67
Author(s):  
SMITA KUMBHAR ◽  
VINOD MATOLE ◽  
YOGESH THORAT ◽  
ANITA SHEGAONKAR ◽  
AVINASH HOSMANI
Keyword(s):  
Spectrophotometric Method ◽  
Method Development ◽  
Pharmaceutical Formulations ◽  
Uv Spectrum ◽  
Colored Complex ◽  
Pharmaceutical Dosage Forms ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Development And Validation

Objective: A new, simple, sensitive, precise and reproducible UV visible spectrophotometric method was developed for the determination of Imatinib in pharmaceutical formulations with alizarin. Methods: The method is based on formation of yellow-colored complex. The UV spectrum of Imatinib in methanol showed λ max at 431 nm. Beer’s law is valid in the concentration range of 10-70 μg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 10-70 μg/ml with regression equation y =0.013x-0.017 and regression correlation coefficient r2= 0.997. Moreover, the method was found to be highly sensitive with LOD (4.3μg/ml) and LOQ (13.07μg/ml). Conclusion: Based on results the proposed method can be successfully applied for the assay of Imatinib in various pharmaceutical dosage forms.

scholarly journals UV-SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF IPRATROPIUM BROMIDE IN API AND PHARMACEUTICAL DOSAGE FORM

2020 ◽  
pp. 69-73
Author(s):  
ANJALI P. KOKANE ◽  
VARSHA S. TEGELI ◽  
BHAGYASHRI S. SHINDE
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Ipratropium Bromide ◽  
Method Development ◽  
Specific Method ◽  
Pharmaceutical Dosage Form ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Method Development And Validation ◽  
Development And Validation

Objective: The current work intended towards the developed and validated by using Simple, rapid, sensitive, precise and specific method UV Spectrophotometric method for the estimation of Ipratropium bromide in API and pharmaceutical formulation. Methods: Water used as a solvent and the absorbance of the drug was measured at the absorbance’s maxima of Ipratropium bromide λmax is 214 nm. Result: Calibration curve plotted in concentration range 20-120µg/ml exhibit the linearity relationship with line equation y=0.0062x+0.3161 and r2=0.995. The Accuracy was found to be 99.5-100.1%, the precision %RSD= 0.12888-0.30533, and the LOD and LOQ is 8.78266-28.5881. The method was found to comply with all the validation parameters as per ICH guidelines indicating the sensitivity of the method towards analyte. Conclusion: The method can be used satisfactory for the routine analysis of Ipratropium Bromide present in API and Pharmaceutical dosage form.

Method Development and Validation of Evening Primrose oil and its Pharmaceutical dosage form by Simple UV- Spectrophotometric Method

2021 ◽  
pp. 133-138
Author(s):  
Shaheen Begum ◽  
Jahnavi. B ◽  
Nameera Jabeen ◽  
Umema Naaz. T ◽  
Sandhya. M
Keyword(s):  
Dosage Form ◽  
Method Development ◽  
Quantitative Estimation ◽  
Spectroscopic Method ◽  
Evening Primrose Oil ◽  
Pharmaceutical Dosage Form ◽  
Evening Primrose ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Development And Validation

In the present work, simple and sensitive UV spectroscopic method has been developed for the quantitative estimation of Evening Primrose Oil in efem capsule. Cyclohexane was used as solvent. In the range of 100-500μg/ml, the linearity of Evening Primrose Oil shows a correlation co-efficient of 0.999. The UV detection wavelength was 233nm. The percentage RSD for precision and accuracy of the method was found to be less than 2%(LOQ) of 23μg/ml and (LOD) of 7.5μg/ml. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Evening Primrose Oil and in formulation dosage form.

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