UV Spectrophotometric Method Development and Validation for the Estimation of Gliclazide in Bulk and Pharmaceutical Dosage Form

2016 ◽  
Vol 6 (3) ◽  
pp. 143 ◽  
Author(s):  
Saroj Kumar Raul ◽  
Bukkuru Spandana ◽  
Patibandla Sameera ◽  
Vegiraju Vikitha
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Pharmaceutical Dosage Form ◽  
Method Development And Validation ◽  
Development And Validation

scholarly journals UV-SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF IPRATROPIUM BROMIDE IN API AND PHARMACEUTICAL DOSAGE FORM

2020 ◽  
pp. 69-73
Author(s):  
ANJALI P. KOKANE ◽  
VARSHA S. TEGELI ◽  
BHAGYASHRI S. SHINDE
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Ipratropium Bromide ◽  
Method Development ◽  
Specific Method ◽  
Pharmaceutical Dosage Form ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Method Development And Validation ◽  
Development And Validation

Objective: The current work intended towards the developed and validated by using Simple, rapid, sensitive, precise and specific method UV Spectrophotometric method for the estimation of Ipratropium bromide in API and pharmaceutical formulation. Methods: Water used as a solvent and the absorbance of the drug was measured at the absorbance’s maxima of Ipratropium bromide λmax is 214 nm. Result: Calibration curve plotted in concentration range 20-120µg/ml exhibit the linearity relationship with line equation y=0.0062x+0.3161 and r2=0.995. The Accuracy was found to be 99.5-100.1%, the precision %RSD= 0.12888-0.30533, and the LOD and LOQ is 8.78266-28.5881. The method was found to comply with all the validation parameters as per ICH guidelines indicating the sensitivity of the method towards analyte. Conclusion: The method can be used satisfactory for the routine analysis of Ipratropium Bromide present in API and Pharmaceutical dosage form.

UV-SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF SAXAGLIPTIN IN API AND PHARMACEUTICAL DOSAGE FORM

2020 ◽  
pp. 63-66
Author(s):  
BHAGYASHRI S. SHINDE ◽  
M. S. KALSHETTI ◽  
ANJALI P. KOKANE
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Pharmaceutical Formulation ◽  
Pharmaceutical Dosage Form ◽  
Ich Guidelines ◽  
Recovery Study ◽  
Method Development And Validation ◽  
Curve Method ◽  
Development And Validation

Objective: A new sensitive, economical, simple, rapid UV spectrophotometric method has been developed for the estimation of Saxagliptin in API and pharmaceutical formulation. Methods: Calibration curve method was used for the estimation of Saxagliptin in API and pharmaceutical formulation. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD, LOQ. Linearity was found in the range of 10-60µg/ml. Accuracy was performed by using a recovery study. The amount of drug recovered was found to be in the range of 99.01-100.1%. All the parameters were validated as per the ICH guidelines. Conclusion: This method is suitable forused routine analysis of present Saxagliptin in API and Pharmaceutical dosage form.

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