scholarly journals UV VISIBLE SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF DASATINIB IN BULK AND SOLID DOSAGE FORM

2020 ◽  
pp. 90-93
Author(s):  
SAILI MADUR ◽  
VINOD MATOLE ◽  
MALLINATH KALSHETTI
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Uv Spectrum ◽  
Solid Dosage ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Uv Visible ◽  
Development And Validation ◽  
Tablet Dosage

Objective: A new, simple, sensitive, precise, reproducible UV visible spectrophotometric method was developed for the determination of Dasatinib in Tablet dosage form with­­­ methanol. Methods: The method is based on the formation of a colorlesscomplex. The UV spectrum of Dasatinib in methanol showed maximum wavelength at 248 nm. Beer’s law is valid in the concentration range of 7-35µg/ml. this method was validated for linearity, accuracy, precision, assay, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 7-35µg/ml with the regression equation y = 0.0332x+0.0633 and regression coefficient i.e. r2= 0.9994moreover, the method was found to be highly sensitive with LOD(1.08µg/ml) and LOQ(3.29µg/ml). Conclusion: Based on the results the proposed method can be successfully applied for the assay of Dasatinib in various tablet dosage forms.

UV Spectrophotometric Method Development and Validation of Carbimazole in Bulk and Tablet Dosage form

2021 ◽  
pp. 163-166
Author(s):  
Zainab A. Bagalkote ◽  
Ganesh Gajeli
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Uv Spectrum ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Uv Visible ◽  
Development And Validation ◽  
Tablet Dosage ◽  
The Given

Objective: A new, simple, sensitive, precise, reproducible UV visible spectrophotometric method was developed for the estimation of Carbimazole in Tablet dosage form. Methods: The UV spectrum of Carbimazole in methanol and distilled water (30:70) showed λ max at 289.6nm. Beer’s law is valid in the concentration range of 10-50µg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 10-50µg/ml with the regression equation y = 0.0232x + 0.0466, and regression coefficient i.e, r2= 0.9992 moreover, the method was found to be highly sensitive with LOD (1.818µg/ml) and LOQ (5.509µg/ml). Conclusion: From the results it can be concluded that the given method can be successfully applied for assay of Carbimazole in Tablet dosage form.

UV Spectrophotometric Method Development and Validation of Darunavir in bulk and Solid Dosage Form

2021 ◽  
pp. 3262-3264
Author(s):  
Varsha Tegeli ◽  
Avinash Birajdar ◽  
Vinod Matole
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Uv Spectrum ◽  
Solid Dosage ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Tablet Dosage

Objective: A new, simple, sensitive, precise, reproducible UV visible spectrophotometric method was developed for the determination of Darunavir in Tablet dosage form with¬¬¬ 0.1N HCl. Method: The method is based on the formation of a colorless complex. The UV spectrum of Darunavir in 0.1N HCl showed maximum wavelength at 298nm. Beer’s law is valid in the concentration range of 10-60µg/ml. this method was validated for linearity, accuracy, precision, assay, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 10-60µg/ml with the regression equation y=0.0113x+0.0098, and regression coefficient i.e. r2=0.9992 moreover, the method was found to be highly sensitive with LOD (1.85µg/ml) and LOQ (5.62µg/ml). Conclusion: Based on the results the proposed method can be successfully applied for the assay of Darunavir in various tablet dosage forms.

UV Spectrophotometric Method Development and Validation of Lignocaine Hydrochloride in Bulk and Semisolid Dosage Form

2021 ◽  
pp. 5280-5282
Author(s):  
Suyash Ingle ◽  
Varsha Tegeli ◽  
Avinash Birajdar ◽  
Vinod Matole ◽  
Sagar Adlinge ◽  
...  
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Spectroscopic Method ◽  
Uv Spectrum ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Development And Validation ◽  
The Given ◽  
Regression Correlation

Objective: A new, simple, sensitive, precise and reproducible UV spectroscopic method was developed for the estimation of Lignocaine Hydrochloride in bulk and Semisolid Formulation. Methods: The UV spectrum of Lignocaine Hydrochloride in RO water showed λ max at 228.8nm. Beer’s law is valid in the concentration range of 20-100µg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 20-100µg/ml with regression equation y = 0.0097x + 0.023 and regression correlation coefficient r2= 0.9992. Moreover, the method was found to be highly sensitive with LOD (3.55μg/ml) and LOQ (10.75μg/ml). Conclusion: Depending on results the given method can be successfully applied for assay of Lignocaine Hydrochloride in Semisolid formulation.

scholarly journals VISIBLE SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF IMATINIB IN BULK AND FORMULATION

2020 ◽  
pp. 63-67
Author(s):  
SMITA KUMBHAR ◽  
VINOD MATOLE ◽  
YOGESH THORAT ◽  
ANITA SHEGAONKAR ◽  
AVINASH HOSMANI
Keyword(s):  
Spectrophotometric Method ◽  
Method Development ◽  
Pharmaceutical Formulations ◽  
Uv Spectrum ◽  
Colored Complex ◽  
Pharmaceutical Dosage Forms ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Development And Validation

Objective: A new, simple, sensitive, precise and reproducible UV visible spectrophotometric method was developed for the determination of Imatinib in pharmaceutical formulations with alizarin. Methods: The method is based on formation of yellow-colored complex. The UV spectrum of Imatinib in methanol showed λ max at 431 nm. Beer’s law is valid in the concentration range of 10-70 μg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 10-70 μg/ml with regression equation y =0.013x-0.017 and regression correlation coefficient r2= 0.997. Moreover, the method was found to be highly sensitive with LOD (4.3μg/ml) and LOQ (13.07μg/ml). Conclusion: Based on results the proposed method can be successfully applied for the assay of Imatinib in various pharmaceutical dosage forms.

scholarly journals New Smartphone Based Colorimetric Method Development and Validation of Emtricitabine in Bulk and Tablet Dosage Form

2021 ◽  
Vol 11 (4) ◽  
pp. 35-40
Author(s):  
Yashashree Mane ◽  
Rajashree Mashru
Keyword(s):  
Dosage Form ◽  
Method Development ◽  
Colorimetric Method ◽  
Uv Spectrophotometry ◽  
Statistical Tool ◽  
Method Development And Validation ◽  
Uv Visible ◽  
Colour Development ◽  
Development And Validation ◽  
Tablet Dosage

The novel smartphone based colorimetric application called PhotoMetrix-PRO has been used for quantitative analysis of Emtricitabine in bulk and tablet dosage form and it compared with conventional UV –visible spectrophtotometry. Smartphone based colorimetric method and UV method was based on detection of intensity of color with increasing concentration Ammonium metavanadate (inorganic oxidizing agent) which was used for preparation Mandeline reagent they used as coloring agent for colour development .The reagent is orange red colour but when it react with Emtricitabine it convert into green color complex. The developed methods have good linearity 50-250μg/ml. The color intensity of API increase with increasing concentration. All images were captured through mobile phone and analysed in PhotoMetrix PRO application. This application convert image into Red, Green and Blue(RGB)histogram and regression models were built into PhotoMetrix-PRO Application both the method have correlation coefficient R2>0.995.The LOD and LOQ for PhotoMetrix PRO  4.77μg/ml and14.92μg/ml and UV-visible spectrophotometry method 4.99 μg/ml and 14.96 μg/ml respectively. The % RSD of Emtricitabine by PhotoMetrix PRO and UV method was 99% (%RSD <2). Appling statistical tool i.e paired two test on both the methods result indicate that both methods are equally significant. Keywords: Smartphone, PhotoMetrix PRO, UV Spectrophotometry, RGB

scholarly journals A NEW ROBUST ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF RIBOCICLIB AND LETROZOLE IN SOLID DOSAGE FORM (TABLET)

2021 ◽  
pp. 129-134
Author(s):  
BHAGYALATA SATAPATHY ◽  
CHAITANYA BANGARI
Keyword(s):  
Analytical Method ◽  
Dosage Form ◽  
Method Development ◽  
Simultaneous Estimation ◽  
Solid Dosage Form ◽  
Solid Dosage ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Tablet Dosage

Objective: The objective of the study was to develop a new robust, sensitive, precise, accurate RP-HPLC analytical method and validate for simultaneous estimation of ribociclib and letrozole in solid dosage form (tablet). Methods: The chromatographic separation was carried out on Waters, symmetry C18 (150 mm×4.6 mm with 3.5 μm), mobile phase used was a mixture of buffer and acetonitrile in the ratio of 80:20, with flow rate of 1ml/min and injection volume of 10 μL for the assay. The detection was done using PDA at 260 nm, with run time of 5 min. The retention time for the drugs ribociclib and letrozole was detected to be 2.648 min and 3.151 min, respectively. The method was validated according to ICH guidelines. Results: The linearity of letrozole and ribociclib was observed to be in the range of 0.50–7.50 and 40.01–600.15, Correlation coefficient (r2) 0.999 and 0.9983, respectively. Accuracy for ribociclib and letrozole is carried out by repeatable concentrations of 50%, 100%, and 150. Validation factors of robustness and ruggedness were detected to be in limits. Conclusion: The developed method was simple, rapid, and consistent; it can be used for the simultaneous estimation of ribociclib and letrozole tablet dosage form in routine analysis.

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