Assessment of In-vitro culture through Nodal explants of Dendrocalamus hamiltonii

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

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In vitro Regeneration and Callogenesis of Libidibia ferrea

Journal of Experimental Agriculture International ◽

10.9734/jeai/2020/v42i430495 ◽

2020 ◽

pp. 14-24

Author(s):

Daniel da Silva ◽

Angela Maria Imakawa ◽

Kamylla Rosas Vieira Guedes ◽

Flávio Mauro Souza Bruno ◽

Paulo de Tarso Barbosa Sampaio

Keyword(s):

Culture Media ◽

In Vitro Regeneration ◽

Shoot Multiplication ◽

Light Sources ◽

Multiplication Rate ◽

Medicinal Species ◽

Efficient System ◽

Blue Led ◽

Friable Callus

Libidibia ferrea (Fabaceae) is a valuable medicinal species in the Amazon, but as it is a protected plant, collection from natural populations is forbidden. Therefore, establishing an efficient system for in vitro regeneration and to improve callogenesis of this species is desirable. To determine the optimal nutritional factors needed for shoot multiplication and callus induction, different culture media, plant growth regulators and LED light sources were tested. The data were subjected to analysis of variance (ANOVA) and means compared by Tukey’s test at p < 0.05. We observe that explants inoculated in the Murashige and Skoog (MS) media with 0.05 mg L-1 of 6-benzilaminopurine (BAP) and cultivated under red-blue LED induced the highest number of shoots (3.67), number of buds (3.13), multiplication rate (15.67) and shoots length (22.03 mm) when compared with other treatments. MS and B5 media supplemented with 2.21 and 4.42 mg L-1 of 2,4-D induced 100% formation of friable callus cultivated under red-blue LED, demonstrating that the light quality significantly influenced callogenesis. Obtained results confirmed that in vitro regeneration and callogenesis is a useful strategy in the protection of endangered species. In this way, a new renewable source of biomass with high quality plant material is presented aiming at the bioprospecting of seedling extracts and friable callus to obtain secondary metabolites of this medicinal plant.

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In vitro Clonal Multiplication of Two Grape (Vitis spp.) Rootstock Genotypes

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v28i1.37193 ◽

2018 ◽

Vol 28 (1) ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

M Alizadeh ◽

SK Singh ◽

VB Patel ◽

PS Deshmukh

Keyword(s):

In Vitro Regeneration ◽

Shoot Multiplication ◽

Nodal Segments ◽

Multiplication Rate ◽

Culture Initiation ◽

Single Node ◽

Clonal Multiplication ◽

Rooting Medium ◽

In Vitro Performance

Single node segments were used to initiate in vitro cultures in two grape rootstocks namely, Dogridge (Vitis champini) and H-144 (Vitis vinifera × V. labrusca). Culture establishment was enhanced using different growth regulators, while BAP was found essential for culture initiation in both genotypes. Less success (38.31%) was obtained in culture establishment of H-144 but it exhibited better vegetative growth and rooting and ex vitro performance as compared to Dogridge. Higher shoot multiplication rate (12 micro-cuttings per culture) was recorded in H-144 while only 9 micro-cuttings per subculture were registered in Dogridge. Addition of activated charcoal to the rooting medium was found beneficial with enhancement of rooting and reduction in time to root initiation in both genotypes. The results suggested that multiplication of these two grape rootstock genotypes can be carried out efficiently by means of direct in vitro regeneration using nodal segments. In vitro performance of these two genotypes was also compared during different stages of micropropagation.Plant Tissue Cult. & Biotech. 28(1): 1-11, 2018 (June)

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In vitro propagation of Camellia fascicularis: a plant species with extremely small populations

Canadian Journal of Plant Science ◽

10.1139/cjps-2019-0027 ◽

2020 ◽

Vol 100 (2) ◽

pp. 202-208

Author(s):

Mengting Wang ◽

Guiliang Zhang ◽

Peiyao Xin ◽

Yun Liu ◽

Bin Li ◽

...

Keyword(s):

Acetic Acid ◽

Large Scale ◽

In Vitro Regeneration ◽

Germplasm Conservation ◽

Shoot Multiplication ◽

Shoot Tips ◽

Naphthalene Acetic Acid ◽

Multiplication Rate ◽

Ms Medium

Camellia fascicularis is an endangered evergreen ornamental plant with pale yellow flowers. An efficient and reproducible in vitro regeneration method is required for its large-scale propagation and germplasm conservation. In this study, one axillary bud per nodal stem was obtained from C. fascicularis cultured on Murashige & Skoog (MS) medium containing 0.1 mg L−1 indole-3-acetic acid (IAA) combined with 1.0 mg L−1 6-benzylaminopurine (BA). Axillary buds from the stem segments were transferred to modified woody plant medium (WPM) supplemented with 3.0 mg L−1 BA in combination with 0.3 mg L−1 IAA for multiplication, thereby resulting in a high shoot multiplication rate of 6.8. Multiple shoots were divided into nodal stems and shoot tips and were induced to root. The shoot tips were induced to root by culturing on one-half MS medium supplemented with 2.0 mg L−1 indole-3-butyric acid (IBA) in combination with 0.3 mg L−1 α-naphthalene acetic acid (NAA), which resulted in 76.0% rooting efficiency with 2.3 roots per shoot. The optimal hormone ratio for inducing rooting of nodal stems was 1.0 mg L−1 IBA in combination with 2.0 mg L−1 NAA, which resulted in 72.7% rooting efficiency with 1.7 roots per nodal stem. These two rooted plantlets were successfully acclimatized and established in a greenhouse.

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Comparison of the effects of growth regulators on in vitro regeneration of ridge gourd and sponge gourd through shoot

Journal of Bio-Science ◽

10.3329/jbs.v18i0.8781 ◽

1970 ◽

Vol 18 ◽

pp. 88-93

Author(s):

E Nahar ◽

ME Haque ◽

B Sikdar

Keyword(s):

Growth Regulators ◽

In Vitro Regeneration ◽

Shoot Multiplication ◽

Shoot Tips ◽

Luffa Cylindrica ◽

Sponge Gourd ◽

Seed Propagation ◽

Luffa Acutangula

Context: Luffa cylindrica and Luffa acutangula are highly cross pollinated crops and propagated mainly by seeds. Genetic stability cannot be maintained easily by seed propagation. It can be maintain by developing special vegetative technique through tissue culture. Objectives: To compare the effects of growth regulators between two species of Luffa using shoot tips for develop the rapid, simple and efficient in vitro regeneration protocol. Materials and Methods: Shoot tips used were collected from in vivo grown plants. They were excised from plants and surface sterilized by HgCl2 treatment. The isolated tips were cultured on semisolid MS medium supplemented with different concentrations and combinations of different growth regulators. Results: The highest result of direct shoot multiplication of ridge gourd was observed using 2 mg/l BAP + 0.2 mg/l GA and in case of sponge gourd it was 1.5 mg/l BAP. For callus induction significant result was found using 4 mg/l BAP + 0.2 mg/l NAA in ridge gourd and 3 mg/l BAP + 0.2 mg/l NAA in sponge gourd. Indirect regeneration was performed by subculturing organogenic callus of sponge gourd on MS with 1.5 mg/l BAP + 0.2 mg/l GA3 and the callus of ridged gourd on MS + 1.5 mg/l BAP + 0.2 mg/l NAA + 0.2 mg/l nicotinic acid. Regenerated shoots of both species were rooted well on MS containing NAA at low concentration. Conclusion: Hormonal differences and simple rapid in vitro regeneration protocol of L. cylindrica and L. acutangula have been established. Keywords: Growth regulators; in vitro regeneration; ridge gourd; sponge gourd. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8781 JBS 2010; 18(0): 88-93

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Augmenting in vitro Shoot Multiplication by Growth Regulators and Photoperiod in Vigna mungo L. (Hepper.)

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2020/v39i3431035 ◽

2020 ◽

pp. 36-45

Author(s):

Soumya Sucharita Singh ◽

Cinmaya Pradhan ◽

Dhaneswar Swain ◽

Gyana Ranjan Rout

Keyword(s):

Crop Improvement ◽

Continuous Light ◽

Shoot Multiplication ◽

Vigna Mungo ◽

Nodal Explants ◽

Multiplication Rate ◽

Semisolid Medium ◽

Apical Meristems ◽

Regenerated Plantlets

An efficient in vitro protocol was developed for the mass multiplication of Vigna mungo vars. PU30 and PU31, an important legume crop through apical meristems and cotyledonary nodal explants. Both apical meristems and cotyledonary nodal explants were cultured on Murashige and Skoog (MS, 1962) medium fortified with 0.5 – 1.50 mg/L 6- benzyl aminopurine (BA) or Kinetin and 3 % (w/v) sucrose. The rate of multiplication was higher when the cultures were incubated under continuous light (24h) than the 16h photoperiod. The average number of multiple shoots per culture was enhanced from 2.43 to 5.46 in the case of var. PU30 and 3.12 to 5.82 in the case of var.PU31 within 4 weeks of culture under 24h photoperiod. The multiplication rate was enhanced till 5th subculture declined thereafter. Rooting was readily achieved upon transferring the shoots to half-strength MS basal semisolid medium supplemented with 0.1– 1.0 mg/L indole-3-butyric acid (IBA) and 2% (w/v) sucrose after 2 weeks of culture. The average number of roots per explants ranged from 3.12 to 5.76. About 80% of regenerated plantlets were hardened in the greenhouse and successfully established in the soil. There is no morphological variation among the regenerated plantlets. This protocol can be used for genetic transformation study for crop improvement of black gram.

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Growth regulator effect on in vitro regeneration of rhododendron cultivars

Horticultural Science ◽

10.17221/643-hortsci ◽

2008 ◽

Vol 35 (No. 2) ◽

pp. 90-94 ◽

Cited By ~ 3

Author(s):

H. Vejsadová

Keyword(s):

Growth Regulators ◽

Growth Regulator ◽

Indoleacetic Acid ◽

In Vitro Regeneration ◽

Shoot Multiplication ◽

Ms Medium ◽

Indolebutyric Acid ◽

Commercial Preparation ◽

High Level

In Rhododendron L. cv. Azuro, Bohumil Kavka, Catharine van Toll, Grandiflorum, Mars, Nova Zembla, Ortrud, Ovation, Prof. Scholz, Purple Splendour, Rebe and Van Werden Poelman, the effect of growth regulators on organogenesis induction of shoot-tip meristems was tested. All cultivars significantly showed the highest shoot regeneration on MS medium containing 6 mg/dm3 isopentenyladenine (2iP). For most rhododendrons, the highest shoot multiplication was found on a medium with 8–10 mg/dm3 2iP in combination with 1 mg/dm3 indoleacetic acid (IAA). Shoots rooted successfully in the substrate with high level of peat without growth regulators. However, the commercial preparation Racine significantly increased rooting in cv. Grandiflorum, Nova Zembla and Rebe compared with 0.03% indolebutyric acid (IBA).

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Effects of Plant Growth Regulators on In vitro Regeneration of Malaysian Indica rice (Oryza sativa L.) cv. MR219 by Shoot Apical Meristem

Asian Journal of Agricultural Research ◽

10.3923/ajar.2012.180.187 ◽

2012 ◽

Vol 6 (4) ◽

pp. 180-187 ◽

Cited By ~ 1

Author(s):

Lavanya Silvarajan ◽

Rosimah Nulit ◽

Faridah Qamaruz Za

Keyword(s):

Oryza Sativa ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Shoot Apical Meristem ◽

Apical Meristem ◽

Oryza Sativa L ◽

Indica Rice ◽

In Vitro Regeneration

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In vitro propagation of Rosa ‘Konstancin’ (R. rugosa × R. beggeriana), a plant with high nutritional and pro-health value

Folia Horticulturae ◽

10.2478/fhort-2018-0022 ◽

2018 ◽

Vol 30 (2) ◽

pp. 259-267 ◽

Cited By ~ 2

Author(s):

Agnieszka Wojtania ◽

Bożena Matysiak

Keyword(s):

In Vitro Propagation ◽

Photosynthetic Activity ◽

Shoot Culture ◽

Bud Break ◽

Multiplication Rate ◽

Shoot Cultures ◽

Old Field ◽

Health Value

Abstract The aim of the study was to develop an efficient micropropagation system for Rosa ‘Konstancin’, an interspecific hybrid between R. rugosa and R. beggeriana, whose fruits have high pro-health value. Shoot cultures were initiated from shoot buds collected in May and August from 15-year-old field-grown Rosa ‘Konstancin’ shrubs. The effect and interaction of different concentrations of phytohormones, sucrose and iron sources on in vitro initiation, multiplication and rooting of shoots were studied. The time of collecting explants from donor plants significantly affected the initiation of shoot culture of Rosa ‘Konstancin’. Considerably higher frequency of bud break (100%) was obtained in explants isolated in August as compared to those collected at the end of May (30%). All buds developed into single shoots after 2-4 weeks of growing on the basal Murashige and Skoog medium containing 2.2 µM BAP, 0.3 µM GA3 and 88 mM of sucrose. The highest multiplication rate (4.8 shoots/explant) in a 5-week period was obtained on MS medium containing 50% of nitrogen salts, 3.1 µM BAP, 0.9 µM GA3 and 58 mM sucrose. High rooting frequency (100%) and quality of rooted plantlets was obtained on a medium containing 0.5 µM IBA, 138 µM Fe-EDDHA and 88 mM sucrose. Fe-EDDHA had a beneficial effect on the growth and photosynthetic activity of Rosa ‘Konstancin’ plantlets, which were successfully acclimatized ex vitro, with a more than 90% survival rate.

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