Identification of differentially expressed genes in prostatic epithelium in relation to androgen receptor CAG repeat length

2006 ◽  
Vol 21 (2) ◽  
pp. 96-105 ◽  
Author(s):  
C.M. Coutinho-Camillo ◽  
E.C. Miracca ◽  
M.L. dos Santos ◽  
S. Salaorni ◽  
A.S. Sarkis ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Differentially Expressed Genes ◽  
Repeat Length ◽  
Cag Repeat ◽  
Differentially Expressed ◽  
Prostatic Epithelium

scholarly journals Identification of Differentially Expressed Genes in Prostatic Epithelium in Relation to Androgen Receptor CAG Repeat Length

2006 ◽  
Vol 21 (2) ◽  
pp. 96-105 ◽  
Author(s):  
C.M. Coutinho-Camillo ◽  
E.C. Miracca ◽  
M.L. Dos Santos ◽  
S. Salaorni ◽  
A.S. Sarkis ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Differentially Expressed Genes ◽  
Repeat Length ◽  
Cag Repeat ◽  
Differentially Expressed ◽  
Cag Repeats ◽  
Pcr Analysis ◽  
Length Variation ◽  
Rt Pcr

The CAG repeat within exon 1 of the androgen receptor (AR) has been associated with the development of prostate cancer. The shorter number of glutamine residues in the protein has been associated with a higher transcriptional activity of the AR and increased relative risk for prostate cancer. In an attempt to identify differentially expressed genes in prostate cancer in relation to AR CAG repeat length variation, in this study we used total mRNA from normal and tumor tissues from 2 prostate cancer patients with AR alleles containing 19 and 26 CAG repeats to perform differential-display RT-PCR analysis. We were able to identify 48 different transcripts that showed homology to several known genes associated with different biological pathways. Among the differentially expressed genes, ATRX and SFRP1 were further validated by quantitative RT-PCR. The transcripts of both ATRX and SFRP1 genes proved to be down-regulated in most of the prostate tumors analyzed by quantitative RT-PCR. Hypermethylation of the promoter region of the SFRP1 gene was found in 17.5% (7/40) of the cases analyzed and was associated with the loss of SFRP1 expression (p=0.014). The differentially expressed genes identified in this study are implicated in several cellular pathways that, when up- or down-regulated, might play a role in the tumorigenic process of the prostate.

Genetic Screening in Infertile Mexican Men: Chromosomal Abnormalities, Y Chromosome Deletions, and Androgen Receptor CAG Repeat Length

2008 ◽  
Vol 29 (6) ◽  
pp. 654-660 ◽  
Author(s):  
S. G. Martinez-Garza ◽  
M. C. Gallegos-Rivas ◽  
M. Vargas-Maciel ◽  
J. M. Rubio-Rubio ◽  
M. E. de los Monteros-Rodriguez ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Y Chromosome ◽  
Genetic Screening ◽  
Chromosomal Abnormalities ◽  
Repeat Length ◽  
Cag Repeat ◽  
Chromosome Deletions ◽  
Mexican Men

Androgen Receptor CAG Repeat Length in Relation to Phenotype Among Females with Nonclassical 21-Hydroxylase Deficiency

2014 ◽  
Vol 47 (07) ◽  
pp. 491-496
Author(s):  
S. Ben-Shachar ◽  
I. Ayalon ◽  
H. Reznik-Wolf ◽  
Y. Tenenbaum-Rakover ◽  
N. Zuckerman-Levin ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Repeat Length ◽  
Cag Repeat ◽  
Hydroxylase Deficiency ◽  
21 Hydroxylase Deficiency

scholarly journals Is the effect of testosterone on body composition modulated by the androgen receptor gene CAG repeat polymorphism in elderly men?

2007 ◽  
Vol 156 (3) ◽  
pp. 395-401 ◽  
Author(s):  
B Lapauw ◽  
S Goemaere ◽  
P Crabbe ◽  
J M Kaufman ◽  
J B Ruige
Keyword(s):  
Body Composition ◽  
Androgen Receptor ◽  
Effect Modification ◽  
Community Dwelling ◽  
Repeat Length ◽  
Fat Free Mass ◽  
Cag Repeat ◽  
Repeat Polymorphism ◽  
Elderly Men ◽  
Body Composition Assessment

Objective: The androgen receptor (AR) gene contains a CAG repeat polymorphism coding for a polyglutamine chain, the length of which is inversely correlated with AR transcriptional activity. We explored whether this polymorphism modulates the activities of testosterone (T) related to body composition in elderly men. Design: We performed cross-sectional analyses using data from a 4-year follow-up study in community-dwelling men aged 75–89 years (n=159). Methods: Body composition was assessed by dual-energy X-ray absorptiometry and its relation with T and the AR gene CAG repeat length was assessed by multiple linear regression analyses, adjusting for confounding and exploring effect modification. Results: AR gene CAG repeat length was not directly related to body composition, either with or without adjustment for confounding variables like age, weight, total T or sex hormone binding globulin (SHBG) levels. However, exploration of effect modification showed that CAG repeat length modulated the relation between T and body composition (standardized regression coefficients of interaction term: β=0.12, P<0.01 and β=−0.09, P<0.05 for fat-free mass and fat mass respectively). These results were confirmed using similar models and data of mean T, SHBG and weight of the 2 years’ preceding body composition assessment instead of data of the same year (β=0.09, P<0.05 and β=−0.09, P<0.05 respectively). Conclusion: These findings suggest that the AR gene CAG polymorphism contributes, albeit modestly, to the between-subject variation of T action on body composition in community-dwelling elderly men.

CAG repeat length in androgen-receptor gene and reproductive variables in fertile and infertile men

The Lancet ◽  
2002 ◽  
Vol 359 (9300) ◽  
pp. 44-46 ◽  
Author(s):  
Ewa Rajpert-De Meyts ◽  
Henrik Leffers ◽  
Jørgen H Petersen ◽  
Anne-Grethe Andersen ◽  
Elisabeth Carlsen ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Receptor Gene ◽  
Androgen Receptor Gene ◽  
Repeat Length ◽  
Cag Repeat ◽  
Infertile Men

scholarly journals Longer CAG repeat length in the androgen receptor gene is associated with premature ovarian failure

2009 ◽  
Vol 24 (12) ◽  
pp. 3230-3235 ◽  
Author(s):  
S. Chatterjee ◽  
R. Singh ◽  
S. Kadam ◽  
A. Maitra ◽  
K. Thangaraj ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Premature Ovarian Failure ◽  
Receptor Gene ◽  
Androgen Receptor Gene ◽  
Ovarian Failure ◽  
Repeat Length ◽  
Cag Repeat

scholarly journals Cag Repeat Number in the Androgen Receptor Gene and Prostate Cancer

2012 ◽  
Vol 15 (1) ◽  
pp. 31-36 ◽  
Author(s):  
S Madjunkova ◽  
A Eftimov ◽  
V Georgiev ◽  
D Petrovski ◽  
A Dimovski ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Cancer Risk ◽  
Receptor Gene ◽  
Prostate Cancer Risk ◽  
Androgen Receptor Gene ◽  
Repeat Length ◽  
Cag Repeat ◽  
Cag Repeats ◽  
Repeat Number

Cag Repeat Number in the Androgen Receptor Gene and Prostate CancerProstate cancer (PC) is the second leading cause of cancer deaths in men. The effects of androgens on prostatic tissue are mediated by the androgen receptor (AR) gene. The 5' end of exon 1 of the AR gene includes a polymorphic CAG triplet repeat that numbers between 10 to 36 in the normal population. The length of the CAG repeats is inversely related to the transactivation function of the AR gene. There is controversy over association between short CAG repeat numbers in the AR gene and PC. This retrospective case-control study evaluates the possible effect of short CAG repeats on the AR gene in prostate cancer risk in Macedonian males. A total of 392 male subjects, 134 PC patients, 106 patients with benign prostatic hyperplasia (BPH) and 152 males from the general Macedonian population were enrolled in this study. The CAG repeat length was determined by fluorescent polymerase chain reaction (PCR) amplification of exon1 of the AR gene followed by capillary electrophoresis (CE) on a genetic analyzer. The mean repeat length in PC patients was 21.5 ±2.65, in controls 22.28 ±2.86 (p = 0.009) and in BPH patients 22.1 ±2.52 (p = 0.038). Short CAG repeats (<19) were found in 21.64% of PC patients vs. 9.43% in BPH patients (p = 0.0154). We also found an association of low Gleason score (<7) with short CAG repeat (<19) in PC patients (p = 0.0306), and no association between the age at diagnosis of PC and BPH and CAG repeat length. These results suggest that reduced CAG repeat length may be associated with increased prostate cancer risk in Macedonian men.

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