Enhanced Production of Fibrinolytic Enzyme from Pseudomonas aeruginosa by Optimization Media Components

Objective: Microorganisms, especially bacteria and its proteins have proven to be potential anti-cancer agents as they selectively attack the tumor cells or tumor micro-environments. The extract of Pseudomonas aeruginosa found to contain proteins that have shown promising anticancer activity. In this work, it was attempted to increase the biomass and trigger the total protein fraction of Pseudomonas aeruginosa (MTCC 2453).Methods: The organism was cultivated in three different such as Luria-Bertani (LB) broth, minimal medium9 (M9), super broth medium (SB) and asparagine-proline (AP) broth. Asparagine proline broth was selected as it has shown high cell growth rate. The media was further optimized by the addition of NaHCO3 and copper sulphate to trigger the protein production. Optimized Aspergine proline broth has achieved highest cell biomass. After the shake flask culture, the overnight grown culture in optimized AP medium was further grown in a 5 L bioreactor by fed-batch cultivation to achieve higher cell densities.Results: The highest protein production was achieved at 40 ° C. Highest biomass and protein content was observed at pH 8 while lowest biomass was produced at pH 2. A gradual increase in biomass content observed from 12 h towards to 48 h.Conclusion: High biomass and proteins content and of Pseudomonas aeruginosa (MTCC 2453) can be produced in optimized asparagine-proline broth. Further the extract is purified to produce novel anti-cancer proteins.

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Optimization of process parameters for the enhanced production of fibrinolytic enzyme by a newly isolated marine bacterium

Bioresource Technology Reports ◽

10.1016/j.biteb.2020.100436 ◽

2020 ◽

Vol 11 ◽

pp. 100436 ◽

Cited By ~ 1

Author(s):

M. Anusree ◽

K. Swapna ◽

C.N. Aguilar ◽

A. Sabu

Keyword(s):

Process Parameters ◽

Marine Bacterium ◽

Fibrinolytic Enzyme ◽

Enhanced Production ◽

Optimization Of Process Parameters

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Enhanced production and characterization of a solvent stable protease from solvent tolerant Pseudomonas aeruginosa PseA

Enzyme and Microbial Technology ◽

10.1016/j.enzmictec.2007.07.019 ◽

2007 ◽

Vol 42 (1) ◽

pp. 11-16 ◽

Cited By ~ 53

Author(s):

Anshu Gupta ◽

S.K. Khare

Keyword(s):

Pseudomonas Aeruginosa ◽

Enhanced Production ◽

Solvent Tolerant ◽

Solvent Stable

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Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme byBacillussp. IND12 Using Response Surface Methodology in Solid-State Fermentation

BioMed Research International ◽

10.1155/2017/3909657 ◽

2017 ◽

Vol 2017 ◽

pp. 1-13 ◽

Cited By ~ 7

Author(s):

Ponnuswamy Vijayaraghavan ◽

P. Rajendran ◽

Samuel Gnana Prakash Vincent ◽

Arumugaperumal Arun ◽

Naif Abdullah Al-Dhabi ◽

...

Keyword(s):

Response Surface Methodology ◽

Solid State ◽

Response Surface ◽

Solid State Fermentation ◽

Enzyme Production ◽

Blood Clot ◽

Fibrinolytic Enzyme ◽

Surface Model ◽

Cow Dung ◽

Enhanced Production

Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings,Bacillussp. IND12 was selected for fibrinolytic enzyme production.Bacillussp. IND12 effectively used cow dung for its growth and enzyme production (687±6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation. Two-level full-factorial experiments revealed that moisture, pH, sucrose, peptone, and MgSO4were the vital parameters with statistical significance (p<0.001). Three factors (moisture, sucrose, and MgSO4) were further studied through experiments of central composite rotational design and response surface methodology. Enzyme production of optimized medium showed4143±12.31 U/g material, which was more than fourfold the initial enzyme production (978±36.4 U/g). The analysis of variance showed that the developed response surface model was highly significant (p<0.001). The fibrinolytic enzyme digested goat blood clot (100%), chicken skin (83±3.6%), egg white (100%), and bovine serum albumin (29±4.9%).

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