scholarly journals Effects of Plant Growth Regulators and Culture Medium Supplements on Embryogenic Callus Induction from Seeds of Zoysiagrass

2009 ◽  
Vol 64 (3-4) ◽  
pp. 239-243 ◽  
Author(s):  
Latiporn Udomsuk ◽  
Kanokwan Jarukamjorn ◽  
Hiroyuki Tanaka ◽  
Waraporn Putalun

Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66 - 100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem explants of P. candollei var. mirifica.


Author(s):  
Nisha Malik ◽  
Rakesh Singh Sengar ◽  
Manoj Kumar Yadav ◽  
Shiv Kumar Singh ◽  
Gopal Singh ◽  
...  

2004 ◽  
Vol 7 (3) ◽  
pp. 331-334 ◽  
Author(s):  
Md. Monirul Islam ◽  
Sanjay Kumar Adhikar . ◽  
Purnendu Gain . ◽  
Md. Mizanur Rahman . ◽  
Noor-e-Alam Siddique .

2015 ◽  
Vol 27 (2) ◽  
pp. 123-134 ◽  
Author(s):  
Ali Pourkhaloee ◽  
Morteza Khosh-Khui

AbstractAn investigation was conducted to study the effects of explant sources, plant growth regulators, carbohydrates and light conditions on indirect cormlet regeneration and the induction of embryogenic callus of freesia (Freesia×hybridaBailey ‘Argenta’). Sections of two different types of explants, corms and pupae (cold storage-produced corms), were placed on Murashige and Skoog (MS) media containing different concentrations of plant growth regulators. The results showed that the highest percentage of callus induction (100%), the highest callus growth (15 mm diameter) and the best type of calli were achieved for pupa explants grown on the medium that contained 4 mg L−11-naphthaleneacetic acid (NAA) and 2 mg L−16-benzylaminopurine (BAP) in the dark. Increasing BAP up to 3 to 4.5 mg L−1resulted in the maximum number of regenerated cormlets from 1 cm2calli (2 cormlets) under light conditions. Overall, the best rooting of regenerated cormlets was achieved on MS media supplemented with 1 mg L−1indole-3-butyric acid (IBA). In the next stage, high quality calli were subcultured on MS media containing sorbitol, sucrose, maltose and mannitol (0, 5, 10 and 15 g L−1). The results indicated that 15 g L−1maltose was able to induce the highest percentage of embryogenic callus, with an average of 88.9% on media containing 2 mg L−1BAP and 1 mg L−1NAA.


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