In vitro studies of alcohol-induced liver injury in virally-infected human hepatocytes: Advantages and limitations

AbstractWe have succeeded in stable mass production of chimeric PXB-mice, whose liver is repopulated by human hepatocytes at a ratio of more than 70%, and we are providing these mice to academia and pharmaceutical companies to support the development of new drugs or studies of liver function. Furthermore, we isolated human hepatocytes, called PXB-cells, from the chimeric mice, and provide them for clients weekly for in vitro studies. In this review, we summarize the existing characterizations of PXB-mice and PXB-cells and their present and future applications.

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Oxidized metabolites of linoleic acid mediated hepatotoxicity as a potential mechanism of dietary unsaturated fat and alcohol induced liver injury: in vivo and in vitro studies (959.20)

The FASEB Journal ◽

10.1096/fasebj.28.1_supplement.959.20 ◽

2014 ◽

Vol 28 (S1) ◽

Author(s):

Huilin Liu ◽

Christopher Ramsden ◽

Ariel Feldstein ◽

Craig McClain ◽

Irina Kirpich

Keyword(s):

Linoleic Acid ◽

Liver Injury ◽

In Vitro Studies ◽

Potential Mechanism ◽

Unsaturated Fat

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The Liver

Bioprinting ◽

10.1093/oso/9780190943547.003.0008 ◽

2021 ◽

pp. 119-137

Author(s):

Kenneth Douglas

Keyword(s):

Liver Injury ◽

Blood Supply ◽

Liver Tissue ◽

Liver Cells ◽

Human Hepatocytes ◽

Human Tissues ◽

Vital Functions ◽

Regenerative Ability ◽

Hybrid Printing

Abstract: This chapter reports on efforts to bioprint liver tissue, including the important types of liver cells and also the liver’s cytoarchitecture—the typical pattern of cellular arrangement within liver tissue. The chapter gives an account of the liver’s remarkable regenerative ability, its over 500 vital functions, its unusual blood supply, and the difficulty of growing liver cells in vitro (in the laboratory). The chapter includes a description of a hybrid printing/casting method employing human hepatocytes (liver cells) encapsulated in a hydrogel called a “liver tissue seed.” Implanted into mice with a liver injury, the seed tissue provided functional support to the failing liver and expanded in size by 50-fold over the course of 11 weeks. The chapter also mentions Organovo, the first commercial bioprinting company and a pioneer in bioprinting commercially available human tissues, notably their lead product, liver tissue.

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Highlight report: prediction of drug induced liver injury (DILI) with human hepatocytes in vitro

Archives of Toxicology ◽

10.1007/s00204-017-2132-5 ◽

2017 ◽

Vol 91 (12) ◽

pp. 4021-4022 ◽

Cited By ~ 3

Author(s):

Wiebke Albrecht

Keyword(s):

Liver Injury ◽

Human Hepatocytes ◽

Drug Induced ◽

Drug Induced Liver Injury

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Protective effects and possible mechanisms of Centella asiatica (L.) urban extract against acute and chronic liver injury: Evidence from in vivo and in vitro studies

Phytotherapy Research ◽

10.1002/ptr.7024 ◽

2021 ◽

Author(s):

Jiabin Lu ◽

Chao Chen ◽

Renhua Gai ◽

Huacheng Qiu ◽

Yue Wu ◽

...

Keyword(s):

Liver Injury ◽

Centella Asiatica ◽

Chronic Liver ◽

In Vitro Studies ◽

Protective Effects ◽

Chronic Liver Injury

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Human hepatocyte-derived extracellular vesicles attenuate the carbon tetrachloride-induced acute liver injury in mice

Cell Death and Disease ◽

10.1038/s41419-021-04204-7 ◽

2021 ◽

Vol 12 (11) ◽

Author(s):

Masatoshi Kakizaki ◽

Yuichiro Yamamoto ◽

Shunya Nakayama ◽

Kazuaki Kameda ◽

Etsuko Nagashima ◽

...

Keyword(s):

Liver Transplantation ◽

Liver Injury ◽

Immune Responses ◽

Extracellular Vesicles ◽

Acute Liver Injury ◽

Human Hepatocytes ◽

Target Cells ◽

Surgical Costs ◽

Chemokine Ligand

AbstractAcute liver injury (ALI) induced by chemicals or viruses can progress rapidly to acute liver failure (ALF), often resulting in death of patients without liver transplantation. Since liver transplantation is limited due to a paucity of donors, expensive surgical costs, and severe immune rejection, novel therapies are required to treat liver injury. Extracellular vesicles (EVs) are used for cellular communication, carrying RNAs, proteins, and lipids and delivering them intercellularly after being endocytosed by target cells. Recently, it was reported that EVs secreted from human hepatocytes have an ability to modulate the immune responses; however, these roles of EVs secreted from human hepatocytes were studied only with in vitro experiments. In the present study, we evidenced that EVs secreted from human hepatocytes attenuated the CCL4-induced ALI by inhibiting the recruitment of monocytes through downregulation of chemokine receptor in the bone marrow and recruitment of neutrophils through the reduction of C-X-C motif chemokine ligand 1 (CXCL1) and CXCL2 expression levels in the liver.

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