HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC - DIODE ARRAY DETECTOR METHOD FOR SIMULTANEOUS ESTIMATION OF ESCITALOPRAM OXALATE AND FLUPENTIXOL DIHYDROCHLORIDE IN COMBINATION

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (08) ◽  
pp. 61-68
Author(s):  
V. V Gawande ◽  
A. V. Chandewar ◽  
Keyword(s):  
High Performance ◽  
Standard Addition ◽  
High Performance Liquid Chromatographic ◽  
Array Detector ◽  
Simultaneous Estimation ◽  
Diode Array ◽  
Diode Array Detector ◽  
Detector Method ◽  
High Degree ◽  
Liquid Chromatographic

The proposed work involves High Performance Liquid Chromatographic – Diode Array Detector method for estimation of escitalopram oxalate and flupentixol dihydrochloride in combination. The analytes were resolved on Waters C18 Xterra column (50 × 4.6 mm id, particle size 3.5 μm) with mobile phase composition of water (containing 0.2 % triethylamine pH adjusted to 2.5 with orthophosphoric acid): acetonitrile: methanol (62:28:10 % v/v/v). The flow rate kept was 1.0 mL/min opting isocratic mode and eluents were tracked down at 230 nm. The retention times for both the drugs were 1.38±0.21 and 2.98±0.41, respectively. Standard addition method was employed for the analysis of flupentixol dihydrochloride. ICH guidelines were followed to validate the method. The method assured linearity in concentration range of 50-150 μg/mL and 2.5-7.5 μg/mL for ESC and FLU, respectively. The method assured high degree of precision and accuracy. The method was proved to be robust by assessing robustness parameters.

scholarly journals Single-Laboratory Validation of a High-Performance Liquid Chromatographic-Diode Array Detector-Fluorescence Detector/Mass Spectrometric Method for Simultaneous Determination of Water-Soluble Vitamins in Multivitamin Dietary Tablets

2009 ◽  
Vol 92 (2) ◽  
pp. 680-688 ◽  
Author(s):  
Pei Chen ◽  
Renata Atkinson ◽  
Wayne R Wolf
Keyword(s):  
Formic Acid ◽  
Dietary Supplements ◽  
High Performance ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Fluorescence Detector ◽  
Liquid Chromatographic ◽  
Single Laboratory

Abstract The purpose of this study was to develop a single-laboratory validated (SLV) method using high-performance liquid chromatography with different detectors diode array detector (DAD); fluorescence detector (FLD); and mass spectrometry (MS) for determination of 7 B-complex vitamins (B1-thiamin, B2-riboflavin, B3-nicotinamide, B6-pyridoxine, B9-folic acid, pantothenic acid, and biotin) and vitamin C in multivitamin/multimineral dietary supplements. The method involves the use of a reversed-phase octadecylsilyl column (4 m, 250 2.0 mm id) and a gradient mobile phase profile. Gradient elution was performed at a flow rate of 0.25 mL/min. After a 5 min isocratic elution at 100 A (0.1 formic acid in water), a linear gradient to 50 A and 50 B (0.1 formic acid in acetonitrile) at 15 min was employed. Detection was performed with a DAD as well as either an FLD or a triple-quadrupole MS detector in the multiple reaction monitoring mode. SLV was performed using Standard Reference Material (SRM) 3280 Multivitamin/Multimineral Tablets, being developed by the National Institute of Standards and Technology, with support by the Office of Dietary Supplements of the National Institutes of Health. Phosphate buffer (10 mM, pH 2.0) extracts of the NIST SRM 3280 were analyzed by the liquid chromatographic (LC)-DAD-FLD/MS method. Following extraction, the method does not require any sample cleanup/preconcentration steps except centrifugation and filtration.

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