scholarly journals Effect of plant growth regulators on two different types of eggplant flowers regarding style length and fruit setting

2021 ◽  
Vol 19 (4) ◽  
pp. e0906-e0906
Author(s):  
Sedighehsadat Khaleghi ◽  

Aim of study: (i) to explore differences between eggplant flowers capable of setting fruit including long (LGs) and medium style flowers (MEs) and those which suffer from severe problems with fertility and fruit setting including short style ones (SRTs); (ii) to study the effect of plant growth regulators on floral morphology and fruit setting. Area of study: Isfahan University of Technology, Isfahan, Iran, 2017 and 2018. Material and methods: First the floral morphology and initial fruit setting of 13 eggplant genotypes from Iran were investigated. Then the differences between LGs and SRTs of two genotypes were explored. Finally, the effect of 1-naphthaleneacetic acid (NAA) and spermidine (Spd) on floral morphology and initial and final fruit setting of these two genotypes was determined. Main results: Results showed SRTs were not capable of fruit setting. Compared to SRTs, LGs had larger central canals, higher protein, total sugar, reducing sugar and K concentrations, as well as longer polar axis and pollen tubes and greater pollen viability. Although 1.5 mM Spd and 20 mg L-1 NAA resulted in increasing of LGs and MEs, and also total initial fruit set, surprisingly, no significant differences were observed in the final yield and final fruit set between the control and these treatments. Researching highlights: Since the rate of fruit dropping was higher in those treatments compared to the control, plants with more SRTs likely regulate their final load by abscising their flowers, and plants with more LGs regulate them by abscising their fruits.

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.


2021 ◽  
Author(s):  
Yuan-yuan Meng ◽  
Shi-jie Song ◽  
Sven Landrein

Abstract Passiflora xishuangbannaensis (Passifloraceae) is endemic to a few sites of Mengyang nature reserve in Yunnan, Xishuangbanna and less than 40 individuals have been recorded. Nine Passiflora species are endemic to Yunnan with most species occurring in South America, making P. xishuangbannaensis highly significant and emblematic to the conservation work in the region. This study is designed to provide the first protocol for in vitro organogenesis and plant regeneration for ex situ conservation and reintroduction for an Asian Passiflora species. Using internodes, petioles and tendrils we optimize calli formation and root elongation using several plant growth regulators, individually or in combination. We also assess the genetic stability of regenerated cells. The maximum callus induction and shoot bud differentiation were both achieved on half Murashige and Skoog basal medium supplemented with 4.44 µM 6-Benzylaminopurine and 1.08 µM 1-Naphthaleneacetic acid. The best rooting was achieved from 30 days old, regenerated shoots on half Murashige and Skoog basal medium supplemented with 1.08 µM 1-Naphthaleneacetic acid. Micropropagated plants were subjected to inter simple sequence repeat markers analyses. Collectively, 86 bands were generated from 6 primers of which 12 bands were polymorphic, showing genetic variation between the regenerated plantlets and the original plant. Response to plant growth regulators was more specific than most other studies using South American species, which could be explained by the morphological and physiological differences between South American and Asian Passiflora species


2021 ◽  
Vol 93 (3) ◽  
Author(s):  
BRUNO CARRA ◽  
MATEUS S. PASA ◽  
EVERTON S. ABREU ◽  
MAXIMILIANO DINI ◽  
CARINA P. PASA ◽  
...  

Author(s):  
Ileana MICLEA ◽  
Rita BERNAT

The aim of the current research was to find the best plant growth regulators for the multiplication of Sarracenia purpurea. Murashige and Skoog medium (MS) was prepared with macronutrients and micronutrients at 1/3 strength, full strength vitamins, supplemented with 30 g/l sucrose and 5 g/l phytagel and autoclaved. After cooling 0.5 mg\l α-naphthaleneacetic acid (NAA), 5 mg\l 6-benzyladenine (BA) or 0.5 mg\l NAA + 3 mg\l BA were added. Young S. purpurea plants were selected and transferred to media with or without plant growth regulators and cultured for 12 weeks. At the end of this time frame number of roots, root length (cm) and number of shoots were evaluated and differences were analysed by the analysis of variance and interpreted using the Tuckey test. The largest number of roots grew in medium supplemented with 0.5 mg\l NAA but the the absence of plant growth regulators increased their length. The best conditions for shoot multiplication were provided by supplementing 1/3MS with 5 mg\l BA.


Author(s):  
Jiequn Ren ◽  
Minghai Zhang ◽  
Li Chen ◽  
Zhinian Li ◽  
Zhangyun Zheng

Author(s):  
Rajpaul Yadav ◽  
Kuldip Kumar ◽  
M. S. Joon ◽  
B. S. Daulta

A field experiment was conducted at Fruit Research Farm of Haryana Agricultural University, Hisar to evaluate the effect of micronutrients and plant growth regulators on fruit cracking and fruit yield in pomegranate cv. Jodhpur Red. The treatments comprising of two micronutrients (H3BO3 and ZnSO4 at 0.20, 0.40, 0.60 %), two growth regulators (2,4-D and NAA at 10, 20, 40 ppm) and water were applied fifteen and thirty days after fruit set. Occurrence of premature fruit cracking was more as compared to mature fruits during both the stages of application. Longitudinal (radial) cracking was more as compared to diagonal (transverse) cracking. 2,4-D and NAA at 10 ppm recorded significantly lower incidence of cracking than all other treatments. Highest fruit yield per acre (50.8 q) was obtained with NAA at 20 ppm when applied fifteen days after fruit set; while 2,4-D at 10 ppm recorded maximum fruit yield when applied thirty days after fruit set.


2015 ◽  
Vol 27 (2) ◽  
pp. 123-134 ◽  
Author(s):  
Ali Pourkhaloee ◽  
Morteza Khosh-Khui

AbstractAn investigation was conducted to study the effects of explant sources, plant growth regulators, carbohydrates and light conditions on indirect cormlet regeneration and the induction of embryogenic callus of freesia (Freesia×hybridaBailey ‘Argenta’). Sections of two different types of explants, corms and pupae (cold storage-produced corms), were placed on Murashige and Skoog (MS) media containing different concentrations of plant growth regulators. The results showed that the highest percentage of callus induction (100%), the highest callus growth (15 mm diameter) and the best type of calli were achieved for pupa explants grown on the medium that contained 4 mg L−11-naphthaleneacetic acid (NAA) and 2 mg L−16-benzylaminopurine (BAP) in the dark. Increasing BAP up to 3 to 4.5 mg L−1resulted in the maximum number of regenerated cormlets from 1 cm2calli (2 cormlets) under light conditions. Overall, the best rooting of regenerated cormlets was achieved on MS media supplemented with 1 mg L−1indole-3-butyric acid (IBA). In the next stage, high quality calli were subcultured on MS media containing sorbitol, sucrose, maltose and mannitol (0, 5, 10 and 15 g L−1). The results indicated that 15 g L−1maltose was able to induce the highest percentage of embryogenic callus, with an average of 88.9% on media containing 2 mg L−1BAP and 1 mg L−1NAA.


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