Content determination of vitexdoin in the fruits of four Vitex species by HPLC method

A sulphurous acid thiolysis-HPLC method for the determination of procyanidins in pine bark products was established. The concentration of sulphurous acid is 1.2%, the concentration of benzyl mercaptan is 2%, the reaction temperature is 90 °C, and the reaction time is 60 minutes. Under these conditions, the preservative rates of catechin, epicatechin, epicatechin gallate and their benzyl sulfide derivatives were 89.7%, 86.2%, 95.4%, 63.1%, 64.6% and 73.5%, respectively. According to this study, the calculation method for the procyanidin content determination by the thiolysis-HPLC method was corrected.

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Content determination of verbascoside in leaves of Rehmannia glutinosa Libosch.by HPLC method

Pharmaceutical Care and Research ◽

10.5428/pcar20150108 ◽

2015 ◽

Vol 15 (1) ◽

pp. 26-46

Author(s):

Liang WANG ◽

QiaoYan ZHANG

Keyword(s):

Hplc Method ◽

Rehmannia Glutinosa ◽

Content Determination

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Content determination of metformin hydrochloride in mice plasma by HPLC method

Pharmaceutical Care and Research ◽

10.5428/pcar20140621 ◽

2014 ◽

Vol 14 (6) ◽

pp. 465-467

Author(s):

SONG DanDan ◽

CAO XiuQin ◽

SHEN JiaWei ◽

ZHOU WeiLi

Keyword(s):

Hplc Method ◽

Metformin Hydrochloride ◽

Content Determination

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Content determination of glycyrrhizin in compound glycyrrhiza oral solution by HPLC method

Pharmaceutical Care and Research ◽

10.5428/pcar20150621 ◽

2015 ◽

Vol 15 (6) ◽

pp. 468-470

Author(s):

QingHua WANG ◽

ShouHong GAO ◽

XiaoJuan XIONG ◽

Feng ZHANG ◽

ZhuoYing HUANG

Keyword(s):

Hplc Method ◽

Oral Solution ◽

Content Determination

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Uncertainty analysis of content determination of icariin in Huangmai mixture by HPLC method

Pharmaceutical Care and Research ◽

10.5428/pcar20120611 ◽

2012 ◽

Vol 12 (6) ◽

pp. 434-437

Author(s):

Li SUN ◽

KeJian SU ◽

GuanQin JIN

Keyword(s):

Uncertainty Analysis ◽

Hplc Method ◽

Content Determination

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DEVELOP A NEW RP-HPLC METHOD AND VALIDATION FOR QUANTITATIVE DRUG CONTENT DETERMINATION OF EZETIMIBE FROM MARKETED FORMULATED TABLETS

International Research Journal of Pharmacy ◽

10.7897/2230-8407.04743 ◽

2013 ◽

Vol 4 (7) ◽

pp. 194-196

Author(s):

Raju Chandra ◽

MD Naushad Alam ◽

Keshav Dutt Sharma ◽

Sujeet Singh ◽

Sanjay Kumar ◽

...

Keyword(s):

Hplc Method ◽

Drug Content ◽

Content Determination ◽

Rp Hplc

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A validated high performance liquid chromatography method for determination of three bioactive compounds p-hydroxy benzoic acid, negundoside and agnuside in Vitex species

Macedonian Journal of Chemistry and Chemical Engineering ◽

10.20450/mjcce.2015.500 ◽

2015 ◽

Vol 34 (2) ◽

pp. 321 ◽

Cited By ~ 1

Author(s):

Tushar Dhanani ◽

Sonal Shah ◽

Satyanshu Kumar

Keyword(s):

Benzoic Acid ◽

High Performance ◽

Limit Of Detection ◽

Solid Phase ◽

Hplc Method ◽

Vitex Negundo ◽

Limit Of Quantification ◽

Chromatography Method ◽

Vitex Species

A validated rapid and simple isocratic HPLC-PDA method was developed for identification and quantification of p- hydroxy benzoic acid and two iridoids negundoside and agnuside in the extracts of two Vitex species, Vitex negundo and Vitex trifolia. The separation of the three compounds was achieved on a RP-18 (250 mm X 4 mm, 5µm) column at 25o C using acetonitrile (15%) and 0.05% trifluoroacetic acid (TFA) in water (85%). Limit of detection (LOD) were 1, 2.5 and 2.5 μg/ml for p- hydroxy benzoic acid, negundoside and agnuside respectively. Similarly, limit of quantification (LOQ) were 2.5, 5 and 5 μg/ml for p- hydroxy benzoic acid, negundoside and agnuside respectively. Good linearity (r2 > 0.999) was observed for all the three compounds in wide concentration range. Using the developed HPLC method, the three compounds were identified and quantified in leaves and bark extracts of Vitex negundo and Vitex trifolia. The novelty of the developed HPLC method is that it does not require complex sample processing such as use of solid phase extraction as well as use of buffer in mobile phase. This is the first report of a validated HPLC method for the simultaneous determination of p- hydroxy benzoic acid, negundoside and agnuside in Vitex species.

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