Isolation and Molecular characterization of Contagious Ecthyma (ORF) Virus from Small Ruminants and Human in Egypt

The aim of this study was to know the epidemiologic characteristics of contagious ecthyma and molecular characterization of the contagious ecthyma virus or ORF virus (ORFV). Sixty four households having 293 goats in Chuadanga district of Bangladesh were surveyed and 30 scab materials were collected from ORF affected goat. Virus detection by PCR was attempted. Sequencing was done for characterization. Goats (n=70) in 15 out of 64 (23.44%) households were found to be infected with ORF. The overall morbidity, mortality and case fatality was 23.89 (70/293), 1.02 (3/293) and 4.29% (3/70), respectively. A proliferative lesion in mouth, lips and gum was found in 100.00% affected goats. Besides, proliferative lesions were also found in legs (19.00%), body (13.00%), and ears (10.00%) of goats. Highest morbidity (29.00%) was found in goats of 7 months-1years aged group and lowest (2.05%) in goats of 2-3 years or more. About 79.00% goats were affected during January-March, 2011 and 21.00% during OctoberDecember, 2010. About 93.33% (28/30) scab samples were found positive by PCR. For molecular characterization PCR amplified viral interlukin-10 (vIL10) gene of 5 different ORFV were sequenced. One virus obtained from sheep and four from goat. Goat isolates were found very closely related (97.20-98.60%) to each other while sheep virus has 95.20-97.20% homology with goat viruses and clustered separately. Our isolates were closely related to Norwegian sheep isolates. It is concluded that ORFV is circulating in Bangladesh with high morbidity but low mortality and all age group of goats are susceptible to this disease. Circulating viruses are closely related to each other.Asian J. Med. Biol. Res. December 2016, 2(4): 555-561

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Identification and molecular characterization of Orf virus in Argentina

Virus Genes ◽

10.1007/s11262-015-1189-6 ◽

2015 ◽

Vol 50 (3) ◽

pp. 381-388 ◽

Cited By ~ 11

Author(s):

Andrea Peralta ◽

Carlos Robles ◽

Agustín Martínez ◽

Lucía Alvarez ◽

Alejandro Valera ◽

...

Keyword(s):

Molecular Characterization ◽

Orf Virus

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Molecular characterization of Brazilian isolates of orf virus

Veterinary Microbiology ◽

10.1016/s0378-1135(99)00151-0 ◽

2000 ◽

Vol 73 (4) ◽

pp. 253-259 ◽

Cited By ~ 29

Author(s):

C Mazur ◽

I.I Ferreira ◽

F.B Rangel Filho ◽

R Galler

Keyword(s):

Molecular Characterization ◽

Orf Virus

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Isolation and molecular characterization of Orf virus from natural outbreaks in goats of Assam

VirusDisease ◽

10.1007/s13337-015-0255-y ◽

2015 ◽

Vol 26 (1-2) ◽

pp. 82-88 ◽

Cited By ~ 7

Author(s):

Mousumi Bora ◽

Durlav Prasad Bora ◽

Nagendra Nath Barman ◽

Biswajyoti Borah ◽

Padma Lochan Bora ◽

...

Keyword(s):

Molecular Characterization ◽

Orf Virus

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First Molecular Characterization of Poxviruses in Cattle, Sheep, and Goats in Botswana

10.21203/rs.3.rs-419694/v1 ◽

2021 ◽

Author(s):

Boitumelo Magret Modise ◽

Tirumala Bharani Kumar Settypalli ◽

Tebogo Kgotlele ◽

Dingrong Xue ◽

Kebonyemodisa Ntesang ◽

...

Keyword(s):

Molecular Characterization ◽

Clinical Symptoms ◽

Phylogenetic Analyses ◽

Small Ruminants ◽

Virus Diseases ◽

Sequence Alignments ◽

Multiple Sequence ◽

Lumpy Skin Disease ◽

Multiple Sequence Alignments

Abstract BackgroundPoxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former presenting also zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the country, poxvirus infections of small ruminants and cattle have received little attention in Botswana, and there was no prior attempt of using molecular tools to diagnose the diseases and characterize the pathogens.MethodsA high-resolution melting (HRM) assay was used to detect and differentiate poxviruses in samples from four cattle (from Mahalapye, Kasane, and Molepolole), one sheep (from Jwaneng), and one goat (from Kasane). Molecular characterization of capripoxviruses and parapoxviruses was undertaken by sequence analysis of RPO30 and GPCR genes.ResultsThe HRM assay revealed lumpy skin disease virus (LSDV) in three cattle samples, pseudocowpox virus (PCPV) in one cattle sample, and orf virus (ORFV) in one goat and one sheep sample. The phylogenetic analyses, based on the RPO30 and GPCR, and the inspection of the multiple sequence alignments showed that the LSDV sequences of Botswana were more like those of common LSDV field isolates encountered in Africa, Asia, and Europe. The Botswana PCPV presented unique features and clustered between camel isolates and cattle isolates of the PCPV group. The Botswana ORFV from goat differed from the ORFV collected in sheep.ConclusionsThis study is the first report on the genetic characterization of pox virus diseases circulating in cattle, goats, and sheep in Botswana. It shows the importance of molecular methods to differentially diagnose pox virus diseases of ruminants.

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Clinical, histopathological, and molecular characterization of Mycoplasma species in sheep and goats in Egypt

Veterinary World ◽

10.14202/vetworld.2021.2561-2567 ◽

2021 ◽

pp. 2561-2567

Author(s):

Walid S. Mousa ◽

Ahmed A. Zaghawa ◽

Ahmed M. Elsify ◽

Mohamed A. Nayel ◽

Zarroug H. Ibrahim ◽

...

Keyword(s):

Molecular Characterization ◽

Respiratory Infections ◽

Histopathological Examination ◽

Small Ruminants ◽

Systemic Reaction ◽

Economic Losses ◽

Mycoplasma Species ◽

Nasal Discharge ◽

Sheep And Goats

Background and Aim: Mycoplasma infection in small ruminants is a serious problem in sheep and goat herds around the world. It is responsible for high economic losses and decreased animal productivity. This study aimed to highlight the clinical, histopathological, minimum inhibitory concentration (MIC), and molecular characterization of Mycoplasma species in sheep and goats in Menoufiya Governorate, Egypt. Materials and Methods: A total of 234 samples were collected; 104 samples were collected from pneumonic lung tissues from the abattoir, in addition, 10 and 20 samples collected from apparently and diseased sheep, respectively, and 40 and 60 samples were collected from apparently and diseased goats, respectively, which were subjected to isolation onto pleuropneumonia-like organism medium. Polymerase chain reaction (PCR), histopathological examination, and determination of the MIC were also performed. Results: Of 104 samples of lung tissues showing pneumonic lesions, 56 (53.84%) were positive for Mycoplasma isolation. The positive isolation of Mycoplasma from 10 and 20 samples from apparently and diseased sheep was 30% and 40%, respectively as well as the positive isolation of Mycoplasma was 17% and 56.66% out of 40 and 60 apparently healthy and diseased field goat's cases, respectively. All the diseased sheep and goats showed respiratory manifestations, including cough, bilateral nasal discharge, conjunctivitis, and systemic reaction. Evaluation of the MIC for Mycoplasma ovipneumoniae revealed that lincospectin and tylosin were the most effective antibiotics at 2.5 μg/mL. Histopathological examination of affected lung tissue showed extensive hemorrhagic pneumonia with extensive alveolar hemorrhage. The PCR technique proved to be a rapid, specific, and sensitive method for the detection of M. ovipneumoniae and Mycoplasma arginini at 390 and 326 bp, respectively. Conclusion: M. ovipneumoniae and M. arginini were the most prevalent species associated with respiratory infections in sheep and goats in the study area. Further studies are needed to investigate the role of these species in dissemination of the disease within herds of small ruminants.

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