Clinical Evaluation of a Rapid Immunochromatographic Serological Method in the Diagnosis of Mycobacterium Tuberculosis

Background: The aim was to determine utility of Cartridge based nucleic acid amplification test (CBNAAT) in diagnosis of mycobacterium tuberculosis in children with neurotuberculosis diagnosed on the basis of clinical evaluation, CSF findings and neuroimaging.Methods: A prospective randomized controlled trial was conducted in Pediatric Department of RNT Medical College, Udaipur, Rajasthan, India from July 2017 to June 2018. Total 110 children of age group of 6 months to 18 years with the diagnosis of tubercular meningoencephalitis (TBME) on the basis of clinical evaluation, CSF examination and neuroimaging were included in the study.Results: A total 110 children were enrolled. Maximum number of cases admitted with TBME were among 1-5 years of age group (60.91%). CSF and gastric aspirate were examined by CBNAAT for MTB. 5 (4.55%) children had CBNAAT positivity in CSF. Gastric aspirate was positive among 16 (14.55%) children. None of the patient had CBNAAT positive result both in CSF and gastric aspirate.Conclusions: TBME is a major health problem in children below 5 years. Gene Xpert assay has the potential to significantly improve and escalate the diagnosis of smear-negative body fluid specimens. CBNAAT for mycobacterium tuberculosis was positive in 5 (4.55%) children from CSF and 16 (14.55%) from gastric aspirate. Negative CBNAAT should not prevent any patient with suspected features of TBME from starting anti tubercular treatment (ATT) as sensitivity of this test remains low. Final judgement to start ATT should be based on clinical, biochemical and radiological profile especially in CNS tuberculosis.

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A multicenter clinical evaluation of Mycobacterium tuberculosis IgG/IgM antibody detection using the colloidal gold method

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-014-2150-7 ◽

2014 ◽

Vol 33 (11) ◽

pp. 1989-1994

Author(s):

Y. Wang ◽

B. Lu ◽

J. Liu ◽

T. Xiao ◽

K. Wan ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Clinical Evaluation ◽

Colloidal Gold ◽

Antibody Detection ◽

Igm Antibody

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Prospective clinical evaluation of Amplicor Mycobacterium tuberculosis PCR test as a screening method in a low-prevalence population.

Journal of Clinical Microbiology ◽

10.1128/jcm.34.8.2001-2003.1996 ◽

1996 ◽

Vol 34 (8) ◽

pp. 2001-2003 ◽

Cited By ~ 6

Author(s):

R Cartuyvels ◽

C De Ridder ◽

S Jonckheere ◽

L Verbist ◽

J Van Eldere

Keyword(s):

Mycobacterium Tuberculosis ◽

Clinical Evaluation ◽

Screening Method ◽

Low Prevalence ◽

Pcr Test

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Clinical Evaluation of BD MAX MDR-TB Assay for Direct Detection of Mycobacterium tuberculosis Complex and Resistance Markers

Journal of Molecular Diagnostics ◽

10.1016/j.jmoldx.2020.06.013 ◽

2020 ◽

Vol 22 (10) ◽

pp. 1280-1286

Author(s):

Sabine Hofmann-Thiel ◽

Sara Plesnik ◽

Marina Mihalic ◽

Marion Heiß-Neumann ◽

Korkut Avsar ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Clinical Evaluation ◽

Mycobacterium Tuberculosis Complex ◽

Direct Detection ◽

Tuberculosis Complex ◽

Mdr Tb ◽

Resistance Markers

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Meropenem-Clavulanic Acid Shows Activity against Mycobacterium tuberculosis In Vivo

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.05690-11 ◽

2012 ◽

Vol 56 (6) ◽

pp. 3384-3387 ◽

Cited By ~ 67

Author(s):

Kathleen England ◽

Helena I. M. Boshoff ◽

Kriti Arora ◽

Danielle Weiner ◽

Emmanuel Dayao ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Clavulanic Acid ◽

Clinical Evaluation ◽

Half Life ◽

Bacterial Burden ◽

Content Type ◽

Term Administration ◽

Poor Stability

ABSTRACTThe carbapenems imipenem and meropenem in combination with clavulanic acid reduced the bacterial burden inMycobacterium tuberculosis-infected macrophages by 2 logs over 6 days. Despite poor stability in solution and a short half-life in rodents, treatment of chronically infected mice revealed significant reductions of bacterial burden in the lungs and spleens. Our results show that meropenem has activity in twoin vivosystems, but stability and pharmacokinetics of long-term administration will offer significant challenges to clinical evaluation.

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