Prospective clinical evaluation of Amplicor Mycobacterium tuberculosis PCR test as a screening method in a low-prevalence population.

There was a concern onMycobacterium tuberculosisspreading to the bone marrow, when it was applied on tuberculous spine infection. This research aimed to study the probability of using autologous bone marrow as a source of mesenchymal stem cell for patients with tuberculous spondylitis. As many as nine patients with tuberculous spondylitis were used as samples. During the procedure, the vertebral lesion material and iliac bone marrow aspirates were obtained for acid fast staining, bacteria culture, and PCR (polymerase chain reaction) tests forMycobacterium tuberculosisat the Clinical Microbiology Laboratory of Faculty of Medicine Universitas Indonesia. This research showed that there was a relationship between diagnostic confirmation of tuberculous spondylitis based on the PCR test and bacterial culture on the solid vertebral lesion material with the PCR test and bacterial culture from the bone marrow aspirates. If the diagnostic confirmation concluded positive results, then there was a higher probability that there would be a positive result for the bone marrow aspirates, so that it was not recommended to use autologous bone marrow as a source of mesenchymal stem cell for patients with tuberculous spondylitis unless the PCR and culture examination of the bone marrow showed a negative result.

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Clinical Evaluation of COBAS TaqMan PCR for the Detection ofMycobacterium tuberculosisandM. aviumComplex

Tuberculosis Research and Treatment ◽

10.1155/2012/170459 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 3

Author(s):

Satoshi Ikegame ◽

Yoritake Sakoda ◽

Nao Fujino ◽

Kazuhito Taguchi ◽

Masayuki Kawasaki ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Observational Study ◽

Careful Consideration ◽

Pcr Analysis ◽

Test Results ◽

Clinical Specimens ◽

Cobas Taqman ◽

Taqman Pcr ◽

Smear Negative ◽

Pcr Test

A retrospective observational study was performed to determine the sensitivity and limitation of PCR test for the detection ofMycobacterium tuberculosisandM. aviumcomplex. We obtained clinical specimens collected from the respiratory tract, culturedM. tuberculosisorM. aviumcomplex, and performed PCR analysis. A total of 299 samples (M. tuberculosis, 177;M. avium, 35;M. intracellulare, 87) were analyzed by COBAS TaqMan PCR from April 2007 to March 2011. The PCR positivity rates were 50–55%, 70–100%, 88–98%, and 100% in smear-negative, smear 1+, 2+, and 3+ groups, respectively. The PCR positivity of tuberculosis in smear 1+ was 80.6%, which was statistically significantly (P<0.001) lower than that of smear 2+ (97.3%). From January 2005 to March 2007, we collected an additional 138 samples (M. tuberculosis, 74;M. avium, 21; M. intracellulare, 43), which were analyzed by COBAS Amplicor PCR. The PCR positivity rates obtained using COBAS TaqMan PCR and COBAS Amplicor PCR were not significantly different. The sensitivity of PCR test for mycobacteria is not sufficient in case of smear 1+. Careful consideration must be given to the interpretation of negative PCR test results in smear 1+, because smear-positive tuberculosis is the criterion for isolation.

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Study on cartridge based nucleic acid amplification test in children with Neurotuberculosis at RNT Medical College, Udaipur, Rajasthan, India

International Journal of Contemporary Pediatrics ◽

10.18203/2349-3291.ijcp20192760 ◽

2019 ◽

Vol 6 (4) ◽

pp. 1588

Author(s):

Shashi Bala ◽

Suresh Goyal

Keyword(s):

Mycobacterium Tuberculosis ◽

Nucleic Acid ◽

Clinical Evaluation ◽

Controlled Trial ◽

Nucleic Acid Amplification Test ◽

Nucleic Acid Amplification ◽

Gastric Aspirate ◽

Age Group ◽

Major Health Problem ◽

Medical College

Background: The aim was to determine utility of Cartridge based nucleic acid amplification test (CBNAAT) in diagnosis of mycobacterium tuberculosis in children with neurotuberculosis diagnosed on the basis of clinical evaluation, CSF findings and neuroimaging.Methods: A prospective randomized controlled trial was conducted in Pediatric Department of RNT Medical College, Udaipur, Rajasthan, India from July 2017 to June 2018. Total 110 children of age group of 6 months to 18 years with the diagnosis of tubercular meningoencephalitis (TBME) on the basis of clinical evaluation, CSF examination and neuroimaging were included in the study.Results: A total 110 children were enrolled. Maximum number of cases admitted with TBME were among 1-5 years of age group (60.91%). CSF and gastric aspirate were examined by CBNAAT for MTB. 5 (4.55%) children had CBNAAT positivity in CSF. Gastric aspirate was positive among 16 (14.55%) children. None of the patient had CBNAAT positive result both in CSF and gastric aspirate.Conclusions: TBME is a major health problem in children below 5 years. Gene Xpert assay has the potential to significantly improve and escalate the diagnosis of smear-negative body fluid specimens. CBNAAT for mycobacterium tuberculosis was positive in 5 (4.55%) children from CSF and 16 (14.55%) from gastric aspirate. Negative CBNAAT should not prevent any patient with suspected features of TBME from starting anti tubercular treatment (ATT) as sensitivity of this test remains low. Final judgement to start ATT should be based on clinical, biochemical and radiological profile especially in CNS tuberculosis.

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