Enhanced Resistance against a Fungal Pathogen Sphaerotheca humuli in Transgenic Strawberry Expressing a Rice Chitinase Gene.

Mitochondria are essential for animal and plant immunity. Here, we report that the effector MoCDIP4 of the fungal pathogen Magnaporthe oryzae targets the mitochondria-associated OsDjA9-OsDRP1E protein complex to reduce rice immunity. The DnaJ protein OsDjA9 interacts with the dynamin-related protein OsDRP1E and promotes the degradation of OsDRP1E, which functions in mitochondrial fission. By contrast, MoCDIP4 binds OsDjA9 to compete with OsDRP1E, resulting in OsDRP1E accumulation. Knockout of OsDjA9 or overexpression of OsDRP1E or MoCDIP4 in transgenic rice results in shortened mitochondria and enhanced susceptibility to M. oryzae. Overexpression of OsDjA9 or knockout of OsDRP1E in transgenic rice, in contrast, leads to elongated mitochondria and enhanced resistance to M. oryzae. Our study therefore reveals a previously unidentified pathogen-infection strategy in which the pathogen delivers an effector into plant cells to target an HSP40-DRP complex; the targeting leads to the perturbation of mitochondrial dynamics, thereby inhibiting mitochondria-mediated plant immunity.

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Transgenic indica rice expressing a bitter melon (Momordica charantia) class I chitinase gene (McCHIT1) confers enhanced resistance to Magnaporthe grisea and Rhizoctonia solani

European Journal of Plant Pathology ◽

10.1007/s10658-009-9501-8 ◽

2009 ◽

Vol 125 (4) ◽

pp. 533-543 ◽

Cited By ~ 22

Author(s):

Ping Li ◽

Yan Pei ◽

Xianchun Sang ◽

Yinghua Ling ◽

Zhenglin Yang ◽

...

Keyword(s):

Rhizoctonia Solani ◽

Magnaporthe Grisea ◽

Indica Rice ◽

Chitinase Gene ◽

Momordica Charantia ◽

Class I ◽

Bitter Melon ◽

Enhanced Resistance

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Mutations in wheat leading to enhanced resistance to the fungal pathogen of yellow rust

Plant Protection Science ◽

10.17221/10324-pps ◽

2002 ◽

Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽

pp. S73-S75

Author(s):

L.A. Boyd ◽

J.A. Howie ◽

T. Worland ◽

R. Stratford ◽

P.H. Smith

Keyword(s):

Fungal Pathogen ◽

Puccinia Striiformis ◽

Yellow Rust ◽

Field Resistance ◽

Deletion Mutants ◽

Molecular Characterisation ◽

Wheat Line ◽

Enhanced Resistance ◽

Negative Effect ◽

Mutant Lines

The isolation and study of plant resistance genes is revealing a story more complicated than the gene-for-gene hypothesis originally implied. The story of resistance is complicated even further by the discovery of genes that appear to have a negative effect on resistance. Early studies in the wheat line Hobbit ‘sib’ identified a number of chromosomes that reduced the level of field resistance to the fungal pathogen Puccinia striiformis f.sp. tritici, the causal agent of yellow rust on wheat. From a series of deletion mutants generated in Hobbit ‘sib’ a number of mutant lines were selected that gave enhanced resistance to yellow rust. The phenotypic, genetic and molecular characterisation of some of these mutants is presented.

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