Plantlet Regeneration from Leaf Explant of Zelkova serrata Makino.

The study was undertaken with a view to investigate the effects of different basal media and organic extracts on organogenesis of Dendrobium orchid. Protocorm like bodies (PLBs) were cultured in Knudson C (KC), Vacin and Went (VW), half strength Murashige and Skoog (Â½MS) and New Phalaenopsis (NP) media supplemented with Sabri banana pulp (Sb), Charcoal (C) and Coconut water (Cw) organic extracts. KC, VW, Â½MS and NP media significantly influenced the plantlet regeneration of Dendrobium orchid. Among the media, Â½MS showed better performance on fresh weight, number PLBs, shoots and leaves per plantlet, The longest shoots were found in KC medium and maximum length of leaves was found in both KC and Â½MS media. In case of different organic extracts Sb showed significantly better performance on fresh weights of PLBs, number of shoot and leaves per explant, length of shoots and leaves. Key words: Organogenesis, Dendrobium, media, organic extracts. DOI = 10.3329/jard.v5i1.1454 J Agric Rural Dev 5(1&2), 30-35, June 2007

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High frequency somatic embryogenesis through leaf explant-derived callus culture in Muscari armeniacum cv. 'Early Giant'

Journal of Plant Biotechnology ◽

10.5010/jpb.2012.39.1.069 ◽

2012 ◽

Vol 39 (1) ◽

pp. 69-74 ◽

Cited By ~ 1

Author(s):

Hyang-Bun Lee ◽

Su-Min Jeon ◽

Mi-Young Chung ◽

Jeung-Sul Han ◽

Chang-Kil Kim ◽

...

Keyword(s):

Somatic Embryogenesis ◽

Callus Culture ◽

High Frequency ◽

Leaf Explant

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In vitro regeneration performance of Corchorus olitorius

Journal of the Bangladesh Agricultural University ◽

10.3329/jbau.v8i1.6390 ◽

1970 ◽

Vol 8 (1) ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

M Hoque ◽

KM Nasiruddin ◽

GKMN Haque ◽

GC Biswas

Keyword(s):

Shoot Regeneration ◽

Callus Induction ◽

Root Formation ◽

Plantlet Regeneration ◽

Ms Medium ◽

Corchorus Olitorius ◽

The Media ◽

Regenerated Plantlets

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the callus induction, shoot regeneration and root formation ability of the cotyledon with attached petiole derived explant of three genotypes viz. O-9897, O-72 and OM-1. The highest callus induction (92.85%) was observed in O-9897 followed by O-72 (82.14%) in the MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. Genotype O-9897 in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA produced the highest percentage of shoot regenerants (83.33%) followed by O-72 (75.00%) in the media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. The root formation from regenerants was the best on halfstrength of MS media supplemented with 0.6 mg/L IBA in genotype O-9897 (45.00%). The in vitro regenerated plantlets from the genotypes O-9897 could be established in the field. Therefore, the genotypes O-9897 of C. olitorius in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA could be used for callus induction and shoot regeneration. Keywords: Regeneration; Phytohormone; Corchorus olitorius DOI: 10.3329/jbau.v8i1.6390J. Bangladesh Agril. Univ. 8(1): 1-6, 2010

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