Protective Activity of Chitosan Nanoparticle against Cadmium Chloride Induced Gastric Toxicity in Rat

Cadmium is a common transition metal that entails an extremely wide range of toxic effects in humans and animals. The cytotoxicity of cadmium ions and its compounds is due to various genotoxic effects, including both DNA damage and chromosomal aberrations. Some bone diseases, kidney and digestive system diseases are determined as pathologies that are closely associated with cadmium intoxication. In addition, cadmium is included in the list of carcinogens because of its ability to initiate the development of tumors of several forms of cancer under conditions of chronic or acute intoxication. Despite many studies of the effects of cadmium in animal models and cohorts of patients, in which cadmium effects has occurred, its molecular mechanisms of action are not fully understood. The genotoxic effects of cadmium and the induction of programmed cell death have attracted the attention of researchers in the last decade. In recent years, the results obtained for in vivo and in vitro experimental models have shown extremely high cytotoxicity of sublethal concentrations of cadmium and its compounds in various tissues. One of the most studied causes of cadmium cytotoxicity is the development of oxidative stress and associated oxidative damage to macromolecules of lipids, proteins and nucleic acids. Brain cells are most sensitive to oxidative damage and can be a critical target of cadmium cytotoxicity. Thus, oxidative damage caused by cadmium can initiate genotoxicity, programmed cell death and inhibit their viability in the human and animal brains. To test our hypothesis, cadmium cytotoxicity was assessed in vivo in U251 glioma cells through viability determinants and markers of oxidative stress and apoptosis. The result of the cell viability analysis showed the dose-dependent action of cadmium chloride in glioma cells, as well as the generation of oxidative stress (p <0.05). Calculated for 48 hours of exposure, the LD50 was 3.1 μg×ml-1. The rates of apoptotic death of glioma cells also progressively increased depending on the dose of cadmium ions. A high correlation between cadmium concentration and apoptotic response (p <0.01) was found for cells exposed to 3–4 μg×ml-1 cadmium chloride. Moreover, a significant correlation was found between oxidative stress (lipid peroxidation) and induction of apoptosis. The results indicate a strong relationship between the generation of oxidative damage by macromolecules and the initiation of programmed cell death in glial cells under conditions of low doses of cadmium chloride. The presented results show that cadmium ions can induce oxidative damage in brain cells and inhibit their viability through the induction of programmed death. Such effects of cadmium intoxication can be considered as a model of the impact of heavy metal pollution on vertebrates.

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Nutrition as an important component of treatment and rehabilitation in cancer patients

HEALTH OF WOMAN ◽

10.15574/hw.2020.149.15 ◽

2020 ◽

pp. 15-22

Author(s):

T.F. Tatarchuk ◽

◽

E.G. Manzhalii ◽

D.V. Pominchuk ◽

T.S. Shevchuk ◽

...

Keyword(s):

Cancer Patients ◽

High Frequency ◽

Nutritional Support ◽

Digestive System ◽

Multidisciplinary Approach ◽

Dietary Factors ◽

Protective Activity ◽

Macro And Micronutrients ◽

Diet And Lifestyle ◽

Cachexia Syndrome

The article deals with the importance of nutritional support for cancer patients. The high frequency of malnutrition and impaired absorption of macro and micronutrients in cancer patients and, which, at its maximum, is anorexia-cachexia syndrome, is the direct cause of the patient’s death. The progression of the tumor process is always accompanied by a violation of nutritional status, which is manifested in changes in weight, weakness, decreased physical activity, and disorders of the digestive system. It is estimated that about half of cancer-related deaths can be prevented, many of which are due to diet and lifestyle. Dietary factors can be involved in the initiation of tumor growth (carcinogens), promote growth, or have protective activity against the development of cancer. Therefore, nutritional support is one of the important components of a multidisciplinary approach in the treatment of cancer. Based on the analysis of the literature, the main tasks of nutritional support, indications, methods and criteria for the effectiveness of therapeutic nutrition are determined for the practitioner, a brief overview of the diets that are used by cancer patients is presented, and a list of anticarcinogenic products is compiled. Key words: anticarcinogenic products, diet, nutritional support, cancer patient, cancer prevention, nutrition.

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