scholarly journals Transgene Expression under the Adenoviral Major Late Promoter, Tripartite Leader Sequence and E1 Genes in Absence and Presence of Adenovirus Infection

2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Mohamed El-Mogy ◽  
Yousef Haj-Ahmad
Keyword(s):  
Transgene Expression ◽  
Leader Sequence ◽  
Adenovirus Infection ◽  
Late Promoter ◽  
Major Late Promoter

scholarly journals 653. The Controlled Transgene Expression in Oncolytic Adenoviral Vectors with Major Late Promoter for Therapy of Cancer

2006 ◽  
Vol 13 ◽  
pp. S251
Author(s):  
Wei Jiang ◽  
Rong Cai ◽  
Guanghua Yang ◽  
Kai Chen ◽  
Yu Chen ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Adenoviral Vectors ◽  
Late Promoter ◽  
Major Late Promoter

scholarly journals Arming Oncolytic Adenoviruses: Effect of Insertion Site and Splice Acceptor on Transgene Expression and Viral Fitness

2020 ◽  
Vol 21 (14) ◽  
pp. 5158
Author(s):  
Martí Farrera-Sal ◽  
Jana de Sostoa ◽  
Estela Nuñez-Manchón ◽  
Rafael Moreno ◽  
Cristina Fillat ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Therapeutic Potential ◽  
Insertion Site ◽  
Viral Fitness ◽  
Late Promoter ◽  
Oncolytic Adenoviruses ◽  
Major Late Promoter ◽  
Insertion Sites ◽  
Main Strategy

Oncolytic adenoviruses (OAds) present limited efficacy in clinics. The insertion of therapeutic transgenes into OAds genomes, known as “arming OAds”, has been the main strategy to improve their therapeutic potential. Different approaches were published in the decade of the 2000s, but with few comparisons. Most armed OAds have complete or partial E3 deletions, leading to a shorter half-life in vivo. We generated E3+ OAds using two insertion sites, After-fiber and After-E4, and two different splice acceptors linked to the major late promoter, either the Ad5 protein IIIa acceptor (IIIaSA) or the Ad40 long fiber acceptor (40SA). The highest transgene levels were obtained with the After-fiber location and 40SA. However, the set of codons of the transgene affected viral fitness, highlighting the relevance of transgene codon usage when arming OAds using the major late promoter.

The major late promoter and bipartite leader sequence of fowl adenovirus

1998 ◽  
Vol 143 (3) ◽  
pp. 537-548 ◽  
Author(s):  
M. Sheppard ◽  
W. Werner ◽  
R. J. McCoy ◽  
M. A. Johnson
Keyword(s):  
Leader Sequence ◽  
Late Promoter ◽  
Fowl Adenovirus ◽  
Major Late Promoter

Spacing requirements for simultaneous recognition of the adenovirus major late promoter TATAAAAG box and initiator element

2005 ◽  
Vol 435 (2) ◽  
pp. 347-362 ◽  
Author(s):  
Delin Ren ◽  
Yuri A. Nedialkov ◽  
Fang Li ◽  
Dianpeng Xu ◽  
Stephan Reimers ◽  
...  
Keyword(s):  
Late Promoter ◽  
Adenovirus Major Late Promoter ◽  
Initiator Element ◽  
Major Late Promoter

Pausing and termination of human RNA polymerase II transcription at a procaryotic terminator

1983 ◽  
Vol 3 (10) ◽  
pp. 1687-1693
Author(s):  
G W Hatfield ◽  
J A Sharp ◽  
M Rosenberg
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Sequence Specificity ◽  
Late Promoter ◽  
Transcription System ◽  
A Cell ◽  
Major Late Promoter ◽  
Dyad Symmetry ◽  
Rna Polymerase Ii Transcription ◽  
Terminator Sequence

Kinetic analyses of runoff transcription in a cell-free eucaryotic transcription system revealed that the bacteriophage lambda 4S RNA terminator caused human RNA polymerase II to pause on the template and partially terminate transcription of transcripts initiated by the adenovirus 2 major late promoter. Analogous to the procaryotic RNA polymerase, the eucaryotic enzyme terminated just beyond the guanine-plus-cytosine-rich region of dyad symmetry in the terminator sequence. These results suggest that the eucaryotic RNA polymerase II may respond to transcription termination sequences similar to those used by the procaryotic enzyme. However, similar templates containing lambda tint or lambda tR1 terminators did not elicit pausing or termination, suggesting that other features, such as sequence specificity, may also be involved.

Downstream sequences affect transcription initiation from the adenovirus major late promoter

1986 ◽  
Vol 6 (7) ◽  
pp. 2684-2694 ◽  
Author(s):  
S L Mansour ◽  
T Grodzicker ◽  
R Tjian
Keyword(s):  
Transcription Initiation ◽  
Simian Virus 40 ◽  
T Antigen ◽  
Control Element ◽  
Coding Region ◽  
Late Promoter ◽  
Sv40 T Antigen ◽  
Transient Expression Assay ◽  
Adenovirus Major Late Promoter ◽  
Major Late Promoter

We analyzed a set of adenovirus-simian virus 40 (SV40) hybrids in which the SV40 T antigen coding sequences are inserted downstream from the adenovirus major late promoter within the first, second, and third segments of the tripartite leader. In infected cells, these viruses give rise to a matched set of hybrid SV40 mRNAs that differ only in the number of tripartite leader segments attached to the complete SV40 T antigen coding region. We found that the number of tripartite leader segments present at the 5' end of the hybrid SV40 mRNAs had little effect on the efficiency of T antigen translation. Surprisingly, insertion of SV40 sequences within the first leader segment, at +33 relative to the start of transcription, significantly reduced the frequency of transcription initiation from the major late promoter. The 3' boundary of this downstream transcriptional control element was mapped between +33 and +190 by showing that insertion of SV40 sequences within the intron after the first leader segment at +190 had very little effect on transcription initiation from the late promoter. A transient expression assay was used to show that the effect of downstream sequences on transcription initiation from the major late promoter is dependent on a trans-acting factor encoded or induced by adenovirus.

Mutations in the adenovirus major late promoter: effects on viability and transcription during infection

1987 ◽  
Vol 7 (3) ◽  
pp. 1091-1100
Author(s):  
L J Brunet ◽  
L E Babiss ◽  
C S Young ◽  
D R Mills
Keyword(s):  
Transcription Initiation ◽  
Experimental System ◽  
Transcription Unit ◽  
Tata Box ◽  
Productive Infection ◽  
Late Promoter ◽  
Lethal Mutant ◽  
Adenovirus Major Late Promoter ◽  
Specific Nucleotide Sequence ◽  
Major Late Promoter

We developed an experimental system to examine the effects of mutations in the adenovirus major late promoter in its correct genomic location during a productive infection. A virus was constructed whose genome could be digested to give a rightward terminal DNA fragment extending from the XhoI site at 22.9 map units, which can be ligated or recombined with plasmid DNA containing adenovirus sequences extending from 0 to 22.9 or 26.5 map units, respectively. Mutations were made by bisulfite mutagenesis in the region between base pairs -52 and -12 with respect to the cap site at +1 and transferred to the appropriate plasmids for viral reconstruction. Of 19 mutant plasmid sequences containing single or multiple G-to-A transitions, 14 could be placed in the viral genome with no apparent change in phenotype. These mutant sequences included those which contained four transitions in the string of G residues immediately downstream of the TATA box. There were no alterations in rates of transcription from the major late promoter, sites of transcription initiation, or steady-state levels of late mRNAs. All of the five mutant sequences which could not be placed in virus contained multiple transitions both up- and downstream of the TATA box. Two of these apparently lethal mutant sequences were used in promoter fusion experiments to test their ability to promote transcription of rabbit beta-globin sequences placed in the dispensable E1 region of the virus. Both sequences showed diminished ability compared with wild-type sequences to promote transcription in this context. Comparisons between these two sequences and the viable mutant sequences suggest a role for the string of G residues located between -38 and -33 in promoting transcription from the major late promoter. The data as a whole also demonstrate that the specific nucleotide sequence of this region of the major late promoter, which overlaps transcription elements of the divergent IVa2 transcription unit and coding sequences of the adenovirus DNA polymerase, is not rigidly constrained but can mutate extensively without loss of these several functions.

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