Cloning and Characterization of a cDNA Encoding Type 1 Diacylglycerol Acyltransferase from Sunflower (Helianthus annuus L.)

2011 ◽  
Vol 66 ◽  
pp. 0063 ◽  
Author(s):  
L. Sun ◽  
C. Ouyang ◽  
S. Kou ◽  
S. Wang ◽  
Y. Yao ◽  
...  
2011 ◽  
Vol 66 (1-2) ◽  
pp. 63-72
Author(s):  
Li Sun ◽  
Chao Ouyang ◽  
Shanglong Kou ◽  
Shenghua Wang ◽  
Yunyi Yao ◽  
...  

A full-length cDNA encoding a putative diacylglycerol acyltransferase (DGAT; EC 2.3.1.20) was obtained from sunflower (Helianthus annuus L.) seeds. The 1524-bp open reading frame of this cDNA, designated as HaDGAT1, encodes a protein of 507 amino acids with a molecular mass of 58.5 kDa showing high homology to DGAT1 enzymes of other plants. The protein characters, such as a predicted structure with a long N-terminal hydrophilic domain followed by 9 transmembrane domains, acyl-CoA-binding signature, diacylglycerol (DAG)-binding and putative endoplasmic reticulum retrieval motifs (ER-DIR), also indicated that HaDGAT belongs to the DGAT1 family. HaDGAT1 is expressed in all plant tissues especially in developing seeds. Expression of recombinant HaDGAT1 in yeast showed an 1.76-fold increase of total fatty acids, especially unsaturated fatty acids such as palmitoleic acid (enhanced by 86.6%) and oleic acid (enhanced by 81.6%).


2016 ◽  
Vol 124 ◽  
pp. 29-37 ◽  
Author(s):  
Anna-Katharina Aschenbrenner ◽  
Moonhyuk Kwon ◽  
Jürgen Conrad ◽  
Dae-Kyun Ro ◽  
Otmar Spring

2011 ◽  
Vol 8 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Salwa N. Zein ◽  
Abd El-khalik ◽  
Khatab Samaa ◽  
A.A.H. Eman ◽  
Clara R. Azzam

2009 ◽  
Vol 70 (9) ◽  
pp. 1117-1122 ◽  
Author(s):  
M. Adrián Troncoso-Ponce ◽  
Nicholas J. Kruger ◽  
George Ratcliffe ◽  
Rafael Garcés ◽  
Enrique Martínez-Force

LWT ◽  
2012 ◽  
Vol 45 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Pablo R. Salgado ◽  
Silvina R. Drago ◽  
Sara E. Molina Ortiz ◽  
Silvana Petruccelli ◽  
Oscar Andrich ◽  
...  

2010 ◽  
Vol 29 (3) ◽  
pp. 239-248 ◽  
Author(s):  
Diego Zavallo ◽  
Marisa Lopez Bilbao ◽  
H. Esteban Hopp ◽  
Ruth Heinz

Plant Science ◽  
2018 ◽  
Vol 272 ◽  
pp. 117-130 ◽  
Author(s):  
M.A. Troncoso-Ponce ◽  
J. Rivoal ◽  
S. Dorion ◽  
R. Sánchez ◽  
M. Venegas-Calerón ◽  
...  

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