scholarly journals Changes in ATPase activity in meristematic cells of Allium cepa L. roots caused by oncostatics

2014 ◽  
Vol 56 (2) ◽  
pp. 201-207
Author(s):  
Maria Podbielkowska ◽  
Maria Wałęza ◽  
Mirosława Ważyńska
Keyword(s):  
Endoplasmic Reticulum ◽  
Atpase Activity ◽  
Allium Cepa ◽  
Cell Respiration ◽  
Meristematic Tissue ◽  
Meristematic Cells ◽  
Allium Cepa L ◽  
Root Apices

Specific changes in the activity of ATPases: mitochondrial and transport associated with the plasmalemma and membranes of endoplasmic reticulum have been observed during experiments on the meristematic tissue of <em>Allium cepa</em> root apices subject to cytostatics (methotrexate, cyclophosphamide, dacarbazine). It seems that an observed increase in the ATPase activity is correlated with a decrease in the level of cell respiration caused by oncostatics.

scholarly journals The suitability of the lead precipitation method for the localization of active ATPases in the meristematic cells of Allium cepa L. roots

2014 ◽  
Vol 56 (2) ◽  
pp. 191-200 ◽  
Author(s):  
Maria Podbielkowska ◽  
Maria Walęza
Keyword(s):  
Allium Cepa ◽  
Plasma Membranes ◽  
Precipitation Method ◽  
Mitochondrial Membranes ◽  
Transport Atpase ◽  
Meristematic Cells ◽  
Allium Cepa L ◽  
Pb Ions ◽  
Root Apices ◽  
The Relationship

Demonstrated in this study is the suitability of the lead precipitation method for the localization of active ATPases bound with the mitochondrial membranes, ER and plasma membranes of meristematic cells of <em>Allium cepa</em> L. root apices. It was found that the presence of glutaraldehyde in the fixative has a significant influence on the fixation of ATPases and that a somewhat smaller influence is exhibited by formaldehyde. Also shown in this study is the relationship between the course of the reaction indicating the presence of active ATPases and the precipitating activity of Pb ions as well as the stimulatory effect of Ca<sup>2</sup>, Mg<sup>2</sup> and K on the transport ATPase bound with the plasma and ER membranes.

scholarly journals Antiproliferative effect of the hydroalcoholic extract of Hymenaea stigonocarpa Mart. ex Hayne (Fabaceae, Caesalpinioideae) on the meristematic cells of Allium cepa L. roots

Biotemas ◽  
2014 ◽  
Vol 28 (1) ◽  
pp. 45 ◽  
Author(s):  
Layana Menezes da Silva ◽  
Ana Paula Peron ◽  
Francisco Ronielson da Silva Carvalho ◽  
Livia Martins ◽  
Iana Felício Bantim Calou ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Antiproliferative Effect ◽  
Hydroalcoholic Extract ◽  
Meristematic Cells ◽  
Allium Cepa L

scholarly journals Influence of extract from shoots of Taxus baccata var. elegantissima on ultrastructure and tubulin cytoskeleton of meristematic cells of Allium cepa L. roots

2014 ◽  
Vol 71 (3) ◽  
pp. 211-221 ◽  
Author(s):  
Agnieszka Majewska ◽  
Mirosława Furmanowa ◽  
Kazimierz Głowniak ◽  
Joanna Guzewska ◽  
Alicja Zobel ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Chromatin Condensation ◽  
Preprophase Band ◽  
Taxus Baccata ◽  
Cell Nuclei ◽  
Root Tips ◽  
Meristematic Cells ◽  
Allium Cepa L ◽  
Interphase Cells ◽  
Average Size

We investigated the influence of extract from <em>Taxus baccata</em> var. Elegantissima (TbE) shoots in 1:8 dilution, containing paclitaxel in concentration of 81,6 µg/g fresh mass on ultrastructure and tubulin cytoskeleton of meristematic cells of <em>Allium cepa</em> L. root tips. Incubation time 3, 6, 12 and 24 h was followed with postincubation in water for 12 and 24 h. During shorter incubation (till 12 h) the surface of the cell nuclei decreased and chromatin became condensed (in comparison to control) but after 24 h the average surface increased and chromatin condensation decreased. In the course of incubation the average size of plastids and vacuoles increased. Moreover, after treatment mitochondria and plastids showed degradation of ultrastructure, which was reversed after 12 h of postincubation. Immunocytochemical assays demonstrated that in the course of incubation in the ThE extract, the tubulin cytoskeleton became partially disorganised. In most interphase cells, cortical microtubules (MTs) lost their oval transverse orientation. The preprophase band (PPB) position in the cell was often asymmetrical. The MTs array of the karyokinetic spindle and phragmoplast was also disturbed. These alterations were completely reversed during postincubation.

Comparison of the Effects of He:Neon and Nd:YAG Laser Radiation on Mitotic Activity of Meristematic Cells of Allium cepa L. Bulbs

1990 ◽  
Vol 8 (6) ◽  
pp. 35-38 ◽  
Author(s):  
M. Fiol de Cuneo ◽  
S. Rubiales de Barioglio ◽  
G. Stutz ◽  
H. Juri ◽  
J. L. Lacuara
Keyword(s):  
Laser Radiation ◽  
Mitotic Activity ◽  
Allium Cepa ◽  
Nd:Yag Laser ◽  
Meristematic Cells ◽  
Allium Cepa L

Effects of anthocyanin-rich extract from red cabbage leaves on meristematic cells of Allium cepa L. roots treated with heavy metals

2007 ◽  
Vol 68 (3) ◽  
pp. 343-350 ◽  
Author(s):  
Sława Glińska ◽  
Magdalena Bartczak ◽  
Sylwia Oleksiak ◽  
Aneta Wolska ◽  
Barbara Gabara ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Allium Cepa ◽  
Red Cabbage ◽  
Meristematic Cells ◽  
Allium Cepa L

scholarly journals Influence of hypoxia on protoplast structure in the plant cell

2015 ◽  
Vol 44 (3) ◽  
pp. 369-376
Author(s):  
Maria Podbielkowska ◽  
Bożena Borys
Keyword(s):  
Endoplasmic Reticulum ◽  
Nucleic Acids ◽  
Allium Cepa ◽  
Plant Cell ◽  
Rough Endoplasmic Reticulum ◽  
Sodium Azide ◽  
Root Tips ◽  
Respiratory Inhibitors ◽  
Meristematic Cells ◽  
Parallel Structures

An influence of hypoxia on the protoplast’s structure in the root tips meristematic cells of onion (<i>Allium cepa</i> L.) and of <i>Tradescantia bracteata</i> Small has been investigated. Hypoxia was caused either by respiratory inhibitors (sodium azide, 2,4-dinitrophenol), phosfon-D or by anaeroibic conditions. In both cases characteristic membranization of cytoplasm was observed. It appeared as spherical and parallel structures of rough endoplasmic reticulum. The observed hypertrophy was not connected with the increase of nucleic acids and proteins synthesis. In the examined cells the membranization was accompanied by an increase of the lipids content.

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