EFFECTS OF DIFFERENT PHYSIOCHEMICAL FACTORS ON REGENERATION OF CYMBOPOGON POLYNEUROS STAPF VIA CALLUS CULTURE AND SUBSEQUENT CHROMOSOMAL STABILITY

In vitro regeneration of Cymbopogon polyneuros Stapf was obtained through callus culture using leaf base, node, and root as explants. Callus was induced from different explants with 2–5 mg/1 α-naphthalene acetic acid (NAA) and 1–2 mg/1 kinetin in Murashige and Skoog's (MS) basal medium. High frequency shoots were noticed from leaf-base callus supplemented with 3.5 mg/1 6-benzylaminopurine (BA), L-arginine, adenine, and a low level of NAA (0.2 mg/1). About 80–85 shoot buds were obtained from ca. 200 mg of callus per culture. The individual shoots produced root in the presence of 0.5–3 mg/1 indole 3-butyric acid or its potassium salt. Regenerated plants were cytologically and phenotypically stable. Regenerants were transplanted into soil and subsequently transferred to the field.

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In vitro Regeneration of Grass Pea (Lathyrus sativus L.)

Jahangirnagar University Journal of Biological Sciences ◽

10.3329/jujbs.v4i2.27790 ◽

2016 ◽

Vol 4 (2) ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

P Saha ◽

M Afrin ◽

AKM Mohiuddin ◽

AM Shohael

Keyword(s):

In Vitro Regeneration ◽

Basal Medium ◽

Multiple Shoots ◽

Nodal Explants ◽

Lathyrus Sativus ◽

Naphthalene Acetic Acid ◽

Grass Pea ◽

Half Strength ◽

Lathyrus Sativus L

In vitro regeneration protocol for grass pea (Lathyrus sativus L.) was optimized using different concentrations and combinations of growth regulators. Direct shoot regeneration obtained through shoot organogenesis from different explants of grass pea cultured on MS medium supplemented with Gamborg B5 vitamin containing 6-benzylaminopurine (BAP), Thidiazuron (TDZ) and ?-naphthalene acetic acid (NAA). Highest percentage of shoots were obtained at 4.0 mg/l of BAP on nodal explants. Stunted multiple shoots were developed from nodal explants while 1.5 mg/l TDZ was used. About 56% of direct shoots were also obtained, while the combination of BAP (4.0 mg/l) and NAA (0.5 mg/l) were used. Regenerated plantlets were rooted most effectively (40%) in rooting medium containing half strength of MS basal medium containing 1.0 mg/l NAA. Well rooted plantlets were further successfully acclimatized to ambient humidity level and grown in controlled environment until hardening.Jahangirnagar University J. Biol. Sci. 4(2): 1-8, 2015 (December)

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Micropropagation of Baptisia `Purple Smoke'

HortScience ◽

10.21273/hortsci.34.2.353 ◽

1999 ◽

Vol 34 (2) ◽

pp. 353-354 ◽

Cited By ~ 1

Author(s):

James R. Ault ◽

Kayri Havens

Keyword(s):

Plant Growth Regulator ◽

Potassium Salt ◽

Shoot Growth ◽

Basal Medium ◽

Shoot Multiplication ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Single Node ◽

Shoot Initiation

Shoot explants from actively growing, greenhouse-maintained plants of Baptisia `Purple Smoke' were cultured in vitro for shoot initiation on Murashige and Skoog (MS) basal medium containing vitamins and supplemented with 30 g·L–1 sucrose, 8.87 μm BA, and 4.14 μm K-IBA. All subsequent media were supplemented with 2.47 mm NaH2PO4 to enhance shoot growth. Single-node explants were subcultured for shoot multiplication on MS medium with either no plant growth regulator or with 2.22, 4.44, 8.87, 17.74, or 35.48 mm BA in combination with 0.0 or 4.14 μm K-IBA. Explants produced a maximum of 4.1 shoots on the medium with 2.22 μm BA. Shoots rooted on all concentrations of K-IBA (2.07, 4.14, 10.36, or 20.72 μm) and K-NAA (2.23, 4.46, 11.15, or 22.29 μm) tested. Maximum rooting was 100% on MS medium with 11.15 μm K-NAA; however, this treatment induced copious stem callusing. Rooted shoots were greenhouse-acclimatized for 2.5 weeks. Overall survival was 86%. For optimal rooting and subsequent acclimatization, treatment with 2.23 μm K-NAA is recommended; this resulted in 83% rooting and 87% acclimatization. Chemical names used: N6 benzyladenine (BA); potassium salt of indole-3-butyric acid (K-IBA); potassium salt of 1-naphthalene acetic acid (K-NAA).

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In vitro Regeneration of the Medicinal Herb of Nilgiri shola, Acmella calva L. from Leaf Derived Callus

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v17i2.2566 ◽

1970 ◽

Vol 17 (2) ◽

pp. 109-114 ◽

Cited By ~ 3

Author(s):

P. Senthilkumar ◽

S. Paulsamy ◽

K. K. Vijayakumar ◽

K. Kalimuthu

Keyword(s):

Key Words ◽

Callus Culture ◽

Callus Induction ◽

Plant Tissue ◽

In Vitro Regeneration ◽

Adventitious Shoots ◽

Basal Medium ◽

Root Initiation ◽

Organogenic Callus

Callus culture was initiated from leaf discs on MS supplemented with BAP, NAA and Kn. The highest frequency (95%) of organogenic callus induction was observed in MS containing BAP at 3.0 mg/l and NAA at 0.3 mg/l. Development of adventitious shoots occurred when the calli were subcultured on MS supplemented with BAP alone at the rate of 3.0 mg/l (80%) and BAP with NAA at the rate of 3.0 and 0.3 mg/l, respectively (95%) and BAP with Kn at the rate of 3.0 and 0.3 mg/l, respectively (70%). The root initiation and the rate of growth were higher in the basal medium containing BAP at 3.0 and 2.5 mg/l. The plantlets were establish successfully in the hardening medium composed by coir pith and soil (1 : 1).Key words: Acmella calva, Leaf derived callus, Nilgiris, IndiaÂ Â Plant Tissue Cult. & Biotech. 17(2): 109-114, 2007Â DOI: 10.3329/ptcb.v17i2.2566

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Optimization of Regeneration Protocol of Rice from Embryo Derived Callus

Progressive Agriculture ◽

10.3329/pa.v18i2.17461 ◽

2013 ◽

Vol 18 (2) ◽

pp. 25-33

Author(s):

N Kamal ◽

KM Nasuruddin ◽

MS Haque ◽

S Yasmin

Keyword(s):

High Frequency ◽

In Vitro Regeneration ◽

Basal Medium ◽

Ms Medium ◽

Rice Cultivars ◽

Regeneration Frequency ◽

Root Regeneration ◽

Maximum Root ◽

High Frequency Transformation

An efficient and reproducible protocol for in vitro regeneration is required to achieve high frequency transformation from transformed calli. We report here high frequency plant regeneration from mature embryonic calli of two BINA rice cultivars Binasail and Binadhan-4. Embryonic calllus initiated on MS basal medium supplemented with 2 mg/l 2, 4-D. Several media with different combinations of growth regulators were tried. Maximum shoot regeneration frequency (63.33%) was observed in Binadhan-4 on MS medium supplemented with 5 mg/l Kinetin + 0.5 mg/l NAA. Maximum root regeneration frequency (70.00%) was observed in Binadhan-4 on MS medium supplemented with 6 mg/l Kinetin + 0.5 mg/l NAA. Well developed plantlets were hardened and transferred to the glasshouse.DOI: http://dx.doi.org/10.3329/pa.v18i2.17461 Progress. Agric. 18(2): 25 - 33, 2007

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In vitro regeneration from leaf-base segments in three genotypes of Urochloa spp.

Crop and Pasture Science ◽

10.1071/cp17395 ◽

2018 ◽

Vol 69 (5) ◽

pp. 527 ◽

Cited By ~ 1

Author(s):

Diliane Harumi Yaguinuma ◽

Luciana Midori Takamori ◽

Adriana Mendonça de Oliveira ◽

Luiz Gonzaga Esteves Vieira ◽

Alessandra Ferreira Ribas

Keyword(s):

In Vitro Regeneration ◽

Forage Grasses ◽

Leaf Base ◽

Breeding Programs ◽

Seed Formation ◽

Regenerated Plants ◽

Tropical Forage ◽

Half Strength ◽

The Media

The key agricultural species of Urochloa P.Beauv. (signal grass), important as tropical forage grasses, are characterised by asexual seed formation (apomixis), and this presents a challenge for breeding programs. Biotechnological approaches could be an option to develop improved cultivars. We evaluated the regenerative potential from three commercial genotypes, U. brizantha cv. Marandu, U. decumbens cv. Basilisk and U. ruziziensis cv. Ruziziensis, by using leaf-base segments as explants. We tested two auxins (2,4-D and picloram) and one cytokinin (TDZ) at four concentrations (1, 2, 3 and 4 mg L–1). Seeds were scarified, peeled and disinfected before inoculation on half-strength MS media in the dark for 14 days. Leaf-base explants were sectioned in thin slices and inoculated into the media. We analysed the number of primary calluses, number of calluses with shoots clusters and the average of regenerated plants. The lowest concentration of auxins tested (1 mg L–1) yielded the highest number of regenerated plants for Marandú and Basilisk, whereas the optimum for Ruziziensis was 2 mg L–1. Medium with higher concentrations of TDZ (4 mg L–1) was required to produce high frequency of plants for all genotypes. Explants cultured on media with TDZ produced very few calluses. These results indicate that the auxins and cytokinin tested can induce plant regeneration from Urochloa leaf-base segments, and may be used to produce transgenic plants in genetic transformation studies.

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High-frequency plant regeneration and histological analysis of callus in Cichorium intybus: An important medicinal plant

Journal of Phytology ◽

10.19071/jp.2016.v8.2980 ◽

2016 ◽

Vol 8 (1) ◽

Cited By ~ 2

Author(s):

K. Dakshayini ◽

C. Vaman Rao ◽

Anitha Karun ◽

U. Bhavyashree ◽

P. Ujwal

Keyword(s):

Acetic Acid ◽

Plant Regeneration ◽

Medicinal Plant ◽

High Frequency ◽

Leaf Disc ◽

Leaf Explants ◽

Cichorium Intybus ◽

Naphthalene Acetic Acid ◽

Regenerated Plants

<p>An efficient in vitro propagation and in vitro flowering protocols were developed for the medicinal plant Cichorium intybus (Asteraceae) using leaf disc explants. Media supplemented with the growth regulator naphthalene acetic acid (NAA) (1.5 mg/l) + 6-benzyle adenine (0.25 mg/l) was used for the initial induction of the callus and further subcultured to the same media for the proliferation of the callus. Pale yellow and green calli were noticed, which depends on incorporation of the growth hormones and their varying concentrations. Murashige and Skoog medium in addition with 2 mg/l kinetin+ 0.5 mg/l indole-3-acetic acid (IBA) + 500 mg/l casein hydrolysate resulted in maximum regeneration. Media supplemented via IBA (0.5 mg/l) and NAA (0.5 mg/l) (98%) was found to be<br />optimum for rhizogenesis for in vitro regenerated plants. For acclimatization 5-6 weeks mature in vitro regenerated plants were transferred into the greenhouse for acclimatization. The histological study revealed the presence actively dividing meristematic cells in callus. The occurrence of the peripheral meristematic zone associated with callus was noticed in after 20 days, which formed the shoot meristems after 45 days of incubation. To our knowledge, this is the first report on high-frequency plant regeneration which was carried out indirectly from the<br />leaf explants which was grown in controlled environment with varying concentration of the growth regulators and histology of callus of different stages from leaf explants of C. intybus.</p>

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The effect of genotype on a barley scutella culture. Histological aspects

Open Life Sciences ◽

10.2478/s11535-012-0113-5 ◽

2013 ◽

Vol 8 (1) ◽

pp. 30-37 ◽

Cited By ~ 1

Author(s):

Halina Ślesak ◽

Grzegorz Góralski ◽

Helena Pawłowska ◽

Barbara Skucińska ◽

Marzena Popielarska-Konieczna ◽

...

Keyword(s):

Histological Analysis ◽

Callus Formation ◽

In Vitro Regeneration ◽

Shoot Bud ◽

Barley Cultivars ◽

Regenerated Plants ◽

Morphogenesis In Vitro ◽

Morphogenic Callus ◽

The Individual

AbstractCereals are known to be recalcitrant to the induction of morphogenesis in vitro and the majority of the methods used are callus-mediated and species/genotype-dependent. In the present investigation, a method of morphogenesis induction from immature scutella of selected barley cultivars was used, and particular attention was paid to histology in the initial stages of the regeneration process in order to confirm whether it occurs directly or indirectly (via callus formation). The length of the period from inoculating scutella on the medium to obtaining plantlets depended on the cultivar and the individual scutellum of the barley and varied between 2.5–4 months. The regeneration efficiency and viability of barley scutella was revealed to be highly genotype dependent. The average number of regenerated plants per regenerating scutellum was highest in the case of cv Granal (3.7). A histological analysis of the cultured explants showed both non-morphogenic and morphogenic callus formation. Two types of indirect morphogenetic response were observed: organogenesis (shoot bud formation) and somatic embryogenesis. This is the first report concerning an analysis of in vitro regeneration from immature scutella of barley cultivars (Stratus, Ryton, Granal and Binal).

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In vitro regeneration of Persian poppy (Papaver bracteatum)

Biologia ◽

10.2478/s11756-010-0079-6 ◽

2010 ◽

Vol 65 (4) ◽

Cited By ~ 4

Author(s):

Sara Rostampour ◽

Haleh Sohi ◽

Ali Dehestani

Keyword(s):

Ascorbic Acid ◽

Callus Induction ◽

High Performance ◽

High Efficiency ◽

In Vitro Regeneration ◽

Naphthalene Acetic Acid ◽

Dichlorophenoxyacetic Acid ◽

Papaver Bracteatum ◽

Callus Proliferation

AbstractPersian poppy (Papaver bracteatum Lindl.) is an important commercial source of medicinal opiates and related compounds. In this research, calli were induced from seeds, roots, cotyledons and hypocotyls of P. bracteatum at a high efficiency. The optimized callus induction media consisted of the Murashige and Skoog (MS) basic media supplemented with 1.0 mg/L 2, 4-dichlorophenoxyacetic acid (2,4-D), 0.1 mg/L kinetin and 15 mg/L ascorbic acid. The concentrations of 2,4-D and ascorbic acid were found critical to callus induction and proliferation. Subsequent subcultures resulted in excellent callus proliferation. Ascorbic acid at concentration 15 mg/L increased the callus proliferation significantly. Maximum callus growth was achieved when the explants were incubated at 25°C. MS salts at full strength were found inhibitory for callus induction, while ľ MS salts were found to favor callus induction. Shoot regeneration of calli in vitro was achieved on ľ MS medium containing 0.5 mg/L benzylamine purine and 1.0 mg/L naphthalene acetic acid. Analysis of alkaloid extracts from Persian poppy tissues by high-performance liquid chromatography showed that thebaine accumulated in the tissues of plants. The thebaine alkaloid profile of the Persian poppy is a well-defined model to evaluate the potential for metabolic engineering of thebaine production in P. bracteatum.

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