scholarly journals Effects of sheep slaughter age on myogenic characteristics in skeletal muscle satellite cells

2022 ◽  
Author(s):  
Yunfei Han ◽  
Wenrui Guo ◽  
Rina Su ◽  
Yanni Zhang ◽  
Le Yang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells ◽  
Slaughter Age

scholarly journals miR-21-5p Regulates the Proliferation and Differentiation of Skeletal Muscle Satellite Cells by Targeting KLF3 in Chicken

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 814
Author(s):  
Donghao Zhang ◽  
Jinshan Ran ◽  
Jingjing Li ◽  
Chunlin Yu ◽  
Zhifu Cui ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Muscle Development ◽  
Target Genes ◽  
Cell Number ◽  
Sequencing Data ◽  
Proliferation Markers ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells ◽  
Proliferation And Differentiation

The proliferation and differentiation of skeletal muscle satellite cells (SMSCs) play an important role in the development of skeletal muscle. Our previous sequencing data showed that miR-21-5p is one of the most abundant miRNAs in chicken skeletal muscle. Therefore, in this study, the spatiotemporal expression of miR-21-5p and its effects on skeletal muscle development of chickens were explored using in vitro cultured SMSCs as a model. The results in this study showed that miR-21-5p was highly expressed in the skeletal muscle of chickens. The overexpression of miR-21-5p promoted the proliferation of SMSCs as evidenced by increased cell viability, increased cell number in the proliferative phase, and increased mRNA and protein expression of proliferation markers including PCNA, CDK2, and CCND1. Moreover, it was revealed that miR-21-5p promotes the formation of myotubes by modulating the expression of myogenic markers including MyoG, MyoD, and MyHC, whereas knockdown of miR-21-5p showed the opposite result. Gene prediction and dual fluorescence analysis confirmed that KLF3 was one of the direct target genes of miR-21-5p. We confirmed that, contrary to the function of miR-21-5p, KLF3 plays a negative role in the proliferation and differentiation of SMSCs. Si-KLF3 promotes cell number and proliferation activity, as well as the cell differentiation processes. Our results demonstrated that miR-21-5p promotes the proliferation and differentiation of SMSCs by targeting KLF3. Collectively, the results obtained in this study laid a foundation for exploring the mechanism through which miR-21-5p regulates SMSCs.

scholarly journals Constitutive Notch Activation Upregulates Pax7 and Promotes the Self-Renewal of Skeletal Muscle Satellite Cells

2012 ◽  
Vol 32 (12) ◽  
pp. 2300-2311 ◽  
Author(s):  
Y. Wen ◽  
P. Bi ◽  
W. Liu ◽  
A. Asakura ◽  
C. Keller ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
The Self ◽  
Self Renewal ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells ◽  
Notch Activation

Identification of miR-2400 gene as a novel regulator in skeletal muscle satellite cells proliferation by targeting MYOG gene

2015 ◽  
Vol 463 (4) ◽  
pp. 624-631 ◽  
Author(s):  
Wei Wei Zhang ◽  
Hui Li Tong ◽  
Xiao Feng Sun ◽  
Qian Hu ◽  
Yu Yang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells

scholarly journals Large-scale isolation of human skeletal muscle satellite cells from post-mortem tissue and development of quantitative assays to evaluate modulators of myogenesis

2013 ◽  
Vol 4 (2) ◽  
pp. 157-169 ◽  
Author(s):  
Ian C. Scott ◽  
Wendy Tomlinson ◽  
Andrew Walding ◽  
Beverley Isherwood ◽  
Iain G. Dougall
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Large Scale ◽  
Human Skeletal Muscle ◽  
Post Mortem ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells

Chronic binge alcohol (CBA) consumption increases cell death and alters myogenic gene expression in skeletal muscle satellite cells

Alcohol ◽  
2013 ◽  
Vol 47 (7) ◽  
pp. 575
Author(s):  
L. Simon ◽  
N. LeCapitaine ◽  
P. Berner ◽  
T. Dodd ◽  
C. Vande Stouwe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Cell Death ◽  
Satellite Cells ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells

Mechanical stretch-induced activation of skeletal muscle satellite cells is dependent on nitric oxide production in vitro

2002 ◽  
Vol 73 (3) ◽  
pp. 235-239 ◽  
Author(s):  
Ryuichi TATSUMI ◽  
Akihito HATTORI ◽  
Ronald E. ALLEN ◽  
Yoshihide IKEUCHI ◽  
Tatsumi ITO
Keyword(s):  
Nitric Oxide ◽  
Skeletal Muscle ◽  
Satellite Cells ◽  
Mechanical Stretch ◽  
Nitric Oxide Production ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells ◽  
Oxide Production
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