scholarly journals Effect of Concentrate Level on the Formation of Conjugated Linoleic Acid and Trans-octadecenoic Acid by Ruminal Bacteria when Incubated with Oilseeds In Vitro

2002 ◽  
Vol 15 (5) ◽  
pp. 687-694 ◽  
Author(s):  
J. H. Wang ◽  
M. K. Song ◽  
Y. S. Son ◽  
M. B. Chang
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Octadecenoic Acid ◽  
Ruminal Bacteria ◽  
Trans Octadecenoic Acid ◽  
Concentrate Level

scholarly journals Duodenal and Milk Trans Octadecenoic Acid and Conjugated Linoleic Acid (CLA) Isomers Indicate that Postabsorptive Synthesis Is the Predominant Source of cis-9-Containing CLA in Lactating Dairy Cows

2002 ◽  
Vol 132 (6) ◽  
pp. 1235-1241 ◽  
Author(s):  
Liliana S. Piperova ◽  
Joseph Sampugna ◽  
Beverly B. Teter ◽  
Kenneth F. Kalscheur ◽  
Martin P. Yurawecz ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Octadecenoic Acid ◽  
Lactating Dairy Cows ◽  
Cla Isomers ◽  
Trans Octadecenoic Acid

Fatty Acid Profile of Canadian Dairy Products with Special Attention to the trans-Octadecenoic Acid and Conjugated Linoleic Acid Isomers

2008 ◽  
Vol 91 (4) ◽  
pp. 811-819 ◽  
Author(s):  
Sanjaya Mendis ◽  
Cristina Cruz-Hernandez ◽  
Walisundera M N Ratnayake
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Fatty Acid Profile ◽  
Dairy Products ◽  
Octadecenoic Acid ◽  
Partial Hydrogenation ◽  
Gas Liquid Chromatography ◽  
Trans Octadecenoic Acid

Abstract Current scientific evidence indicates that consumption of industrial trans fatty acids (TFA) produced via partial hydrogenation of vegetable oils increases the risk of coronary heart disease. However, some studies have suggested that ruminant TFA, especially vaccenic acid (VA or 11t-18:1) and rumenic acid (RA or 9c,11t-18:2), which is a conjugated linoleic acid (CLA) isomer, may have potential beneficial health effects for humans. To date, no concerted effort has been made to provide detailed isomer composition of ruminant TFA and CLA of Canadian dairy products, information that is required to properly assess their nutritional impacts. To this end, we analyzed the fatty acid profile of popular brands of commercial cheese (n = 17), butter (n = 12), milk (n =8), and cream (n = 4) sold in retail stores in Ottawa, Canada, in 20062007 by silver nitrate thin-layer chromatography and gas liquid chromatography. The average total TFA content of cheese, butter, milk, and cream samples were 5.6, 5.8, 5.8, and 5.5 of total fatty acids, respectively. VA was the major trans-octadecenoic acid (18:1) isomer in all the Canadian dairy samples with average levels of (as total trans-18:1) 33.9 in cheese, 35.6 in butter, 31.0 milk, and 30.1 in cream. The different dairy products contained very similar levels of CLA, which ranged from 0.5 to 0.9 of total fat. RA was the major CLA isomer of all the dairy products, accounting for 82.483.2 of total CLA. There were no significant differences (P > 0.05) in the fatty acid profile between the 4 different dairy groups, which suggests lack of processing effects on the fatty acid profile of dairy fat.

Direct Effects of Conjugated Linoleic Acid Isomers on P815 Mast Cells in vitro

2012 ◽  
Vol 41 (4) ◽  
pp. 399-411
Author(s):  
Siddharth Krishnan ◽  
Joshua Russell ◽  
MaryLou Bodziak ◽  
Stephen Koury ◽  
Patricia Masso-Welch
Keyword(s):  
Linoleic Acid ◽  
Mast Cells ◽  
Conjugated Linoleic Acid ◽  
Direct Effects

scholarly journals In silico characterization of linoleic acid biotransformation to rumenic acid in food derived Lactobacillus plantarum YW11

2020 ◽  
Author(s):  
Tariq Aziz ◽  
Abid Sarwar ◽  
Muhammad Fahim ◽  
Sam Al Dalali ◽  
Zia Ud Din ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Lactobacillus Plantarum ◽  
In Silico ◽  
Octadecadienoic Acid ◽  
Scale Up ◽  
Octadecenoic Acid ◽  
Acid Ethyl Ester ◽  
Dependent Manner ◽  
In Silico Characterization

Lactobacillus plantarum YW11 capability to convert linoleic acid into conjugated linoleic acid and other metabolites was studied in a dose-dependent manner by supplementing LA at different concentrations. L. plantarum YW11 displayed a uniform distinctive growth curve of CLA and other metabolites at concentrations of LA ranging from 1% (w/v) to 10% (w/v), with slightly increased growth at higher LA concentrations. The biotransformation capability of L. plantarum YW11 evaluated by GC-MS revealed a total of one CLA isomer, i.e. 9-cis,11-trans-octadecadienoic acid, also known as the rumenic acid (RA), one linoleic acid isomer (linoelaidic acid), and LA metabolites: (E)-9-octadecenoic acid ethyl ester, trans, trans-9,12-octadecadienoic acid, propyl ester and stearic acid. All the metabolites of linoleic acid were produced from 1 to 10% LA supplemented MRS media, while surprisingly the only conjugated linoleic acid compound was produced at 10% LA. To assess the presence of putative enzymes, responsible for conversion of LA into CLA, in silico characterization was carried out. The in silico characterization revealed presence of four enzymes (10-linoleic acid hydratase, linoleate isomerase, acetoacetate decarboxylase and dehydrogenase) that may be involved in the production of CLA (rumenic acid) and LA isomers. The biotransformation ability of L. plantarum YW11 to convert LA into RA has great prospects for biotechnological and industrial implications that could be exploited in the future scale-up experiments.

scholarly journals Mining the Microbiota of the Neonatal Gastrointestinal Tract for Conjugated Linoleic Acid-Producing Bifidobacteria

2004 ◽  
Vol 70 (8) ◽  
pp. 4635-4641 ◽  
Author(s):  
E. Rosberg-Cody ◽  
R. P. Ross ◽  
S. Hussey ◽  
C. A. Ryan ◽  
B. P. Murphy ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Bifidobacterium Bifidum ◽  
Genomic Diversity ◽  
Gas Liquid Chromatography ◽  
Fecal Material ◽  
Single Strain ◽  
Different Strains

ABSTRACT This study was designed to isolate different strains of the genus Bifidobacterium from the fecal material of neonates and to assess their ability to produce the cis-9, trans-11 conjugated linoleic acid (CLA) isomer from free linoleic acid. Fecal material was collected from 24 neonates aged between 3 days and 2 months in a neonatal unit (Erinville Hospital, Cork, Ireland). A total of 46 isolates from six neonates were confirmed to be Bifidobacterium species based on a combination of the fructose-6-phosphate phosphoketolase assay, RAPD [random(ly) amplified polymorphic DNA] PCR, pulsed-field gel electrophoresis (PFGE), and partial 16S ribosomal DNA sequencing. Interestingly, only 1 of the 11 neonates that had received antibiotic treatment produced bifidobacteria. PFGE after genomic digestion with the restriction enzyme XbaI demonstrated that the bifidobacteria population displayed considerable genomic diversity among the neonates, with each containing between one and five dominant strains, whereas 11 different macro restriction patterns were obtained. In only one case did a single strain appear in two neonates. All genetically distinct strains were then screened for CLA production after 72 h of incubation with 0.5 mg of free linoleic acid ml−1 by using gas-liquid chromatography. The most efficient producers belonged to the species Bifidobacterium breve, of which two different strains converted 29 and 27% of the free linoleic acid to the cis-9, trans-11 isomer per microgram of dry cells, respectively. In addition, a strain of Bifidobacterium bifidum showed a conversion rate of 18%/μg dry cells. The ability of some Bifidobacterium strains to produce CLA could be another human health-promoting property linked to members of the genus, given that this metabolite has demonstrated anticarcinogenic activity in vitro and in vivo.

Effect of Dietary Vegetable Oil Supplementation on C18 Fatty Acids and Conjugated Linoleic Acid Production; An In vitro Fermentation Study

2013 ◽  
Vol 12 (6) ◽  
pp. 516-520 ◽  
Author(s):  
Julakorn Panatuk ◽  
Suthipong Uriyapongs ◽  
Chainarong Nawanukraw ◽  
Chirasak Phoemchala ◽  
Pitukpol Pornanake
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Vegetable Oil ◽  
Acid Production ◽  
In Vitro Fermentation

Phytosterols Esterified with Conjugated Linoleic Acid. In Vitro Intestinal Digestion and Interaction on Cholesterol Bioaccessibility

2012 ◽  
Vol 60 (45) ◽  
pp. 11323-11330 ◽  
Author(s):  
Maria I. Moran-Valero ◽  
Diana Martin ◽  
Guzman Torrelo ◽  
Guillermo Reglero ◽  
Carlos F. Torres
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Intestinal Digestion
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