Gene Functional Studies Using Bacterial Artificial Chromosome (BACs)

Gene expression from bacterial artificial chromosome (BAC) clones has been demonstrated to facilitate physiologically relevant levels compared to viral and nonviral cDNA vectors. BACs are large enough to transfer intact genes in their native chromosomal setting together with flanking regulatory elements to provide all the signals for correct spatiotemporal gene expression. Until recently, the use of BACs for functional studies has been limited because their large size has inherently presented a major obstacle for introducing modifications using conventional genetic engineering strategies. The development ofin vivohomologous recombination strategies based on recombineering inE. colihas helped resolve this problem by enabling facile engineering of high molecular weight BAC DNA without dependence on suitably placed restriction enzymes or cloning steps. These techniques have considerably expanded the possibilities for studying functional genetics using BACsin vitroandin vivo.

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Faculty Opinions recommendation of Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1009413.126057 ◽

2002 ◽

Author(s):

Grant McFadden

Keyword(s):

Escherichia Coli ◽

Bacterial Artificial Chromosome ◽

Vaccinia Virus ◽

Mammalian Cells ◽

Infectious Virus ◽

Artificial Chromosome ◽

Virus Genome

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Targeting CreERT2 expression to keratin 8-expressing murine simple epithelia using bacterial artificial chromosome transgenesis

Transgenic Research ◽

10.1007/s11248-012-9598-y ◽

2012 ◽

Vol 21 (5) ◽

pp. 1117-1123 ◽

Cited By ~ 15

Author(s):

Li Zhang ◽

Boyu Zhang ◽

Sang Jun Han ◽

Amy N. Shore ◽

Jeffrey M. Rosen ◽

...

Keyword(s):

Bacterial Artificial Chromosome ◽

Artificial Chromosome ◽

Keratin 8

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Revising the Chromosome-Specific Probes of White Hawk (Leucopternis albicollis)

Proceedings ◽

10.3390/iecge-07152 ◽

2020 ◽

Vol 76 (1) ◽

pp. 1

Author(s):

Ivanete Furo ◽

Rafael Kretschmer ◽

Jorge Pereira ◽

Darren Griffin ◽

Rebecca O’Connor ◽

...

Keyword(s):

Bacterial Artificial Chromosome ◽

Artificial Chromosome ◽

Bird Of Prey ◽

Inaccurate Data ◽

Intrachromosomal Rearrangements

Leucopternis albicollis is a diurnal bird of prey with extensive karyotype reorganization. Chromosome-specific probes from this species have been used successfully to detect intrachromosomal rearrangements in different species of bird since 2010. However, some gaps were detected in this first set of probes. Here, we have obtained a new set of whole chromosome probes in order to improve the previous one; also, we have performed experiments using bacterial artificial chromosome (BAC) from chicken microchromosomes. Our results demonstrated that the microchromosomes were involved in fusion events. In addition, a new nomenclature has been proposed for the new set of probes and some inaccurate data were corrected.

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