Baculovirus Gene Expression

10.5772/56955 ◽  
2013 ◽  
Author(s):  
Marcelo F. ◽  
M. Leticia ◽  
Ricardo Salvador ◽  
Alicia Sciocco ◽  
Vctor Romanowski
Keyword(s):  
Gene Expression ◽  
Baculovirus Gene

A Sensitive Model System for In Vivo Monitoring of Baculovirus Gene Expression in Single Infected Insect Cells

1992 ◽  
Vol 10 (5) ◽  
pp. 565-569 ◽  
Author(s):  
Matti Karp ◽  
Karl Åkerman ◽  
Christer Lindqvist ◽  
Ari Kuusisto ◽  
Petri Saviranta ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Model System ◽  
In Vivo Monitoring ◽  
Baculovirus Gene ◽  
Infected Insect

scholarly journals Establishment of a Large-Scale Purification Procedure for Purified Recombinant Bovine Interferon-τ Produced by a Silkworm-Baculovirus Gene Expression System

2004 ◽  
Vol 66 (11) ◽  
pp. 1395-1401 ◽  
Author(s):  
Hidekazu NAGAYA ◽  
Toshimichi KANAYA ◽  
Hiroki KAKI ◽  
Yoneko TOBITA ◽  
Masashi TAKAHASHI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Large Scale ◽  
Expression System ◽  
Purification Procedure ◽  
Gene Expression System ◽  
Baculovirus Gene

scholarly journals Sequence, transcriptional mapping, and overexpression of p47, a baculovirus gene regulating late gene expression.

1993 ◽  
Vol 67 (5) ◽  
pp. 2513-2520 ◽  
Author(s):  
E B Carstens ◽  
A L Lu ◽  
H L Chan
Keyword(s):  
Gene Expression ◽  
Late Gene ◽  
Late Gene Expression ◽  
Baculovirus Gene ◽  
Transcriptional Mapping

Detection of baculovirus gene expression in insect cells and larvae by low light video image analysis

1996 ◽  
Vol 61 (1-2) ◽  
pp. 151-156 ◽  
Author(s):  
W.H.R. Langridge ◽  
V.I. Krausova ◽  
A.A. Szalay ◽  
I. Fodor
Keyword(s):  
Gene Expression ◽  
Image Analysis ◽  
Insect Cells ◽  
Video Image ◽  
Low Light ◽  
Video Image Analysis ◽  
Baculovirus Gene

Expression of biologically active recombinant porcine GM-CSF by baculovirus gene expression system

1998 ◽  
Vol 76 (3) ◽  
pp. 195-201 ◽  
Author(s):  
S Inumaru ◽  
T Kokuho ◽  
S Denham ◽  
MS Denyer ◽  
E Momotani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Biologically Active ◽  
Gene Expression System ◽  
Gm Csf ◽  
Baculovirus Gene

Identification of lef-7: A Baculovirus Gene Affecting Late Gene Expression

Virology ◽  
1994 ◽  
Vol 200 (2) ◽  
pp. 260-269 ◽  
Author(s):  
Timothy D. Morris ◽  
Jason W. Todd ◽  
Bradford Fisher ◽  
Lois K. Miller
Keyword(s):  
Gene Expression ◽  
Late Gene ◽  
Late Gene Expression ◽  
Baculovirus Gene

Development of a Novel Preparation Method of Recombinant Proteoliposomes Using Baculovirus Gene Expression Systems

2008 ◽  
Vol 144 (6) ◽  
pp. 763-770 ◽  
Author(s):  
H. Fukushima ◽  
M. Mizutani ◽  
K. Imamura ◽  
K. Morino ◽  
J. Kobayashi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Preparation Method ◽  
Expression Systems ◽  
Baculovirus Gene

PRODUCTION OF BIOLOGICALLY ACTIVE RECOMBINANT BOVINE INTERFERON-γ BY TWO DIFFERENT BACULOVIRUS GENE EXPRESSION SYSTEMS USING INSECT CELLS AND SILKWORM LARVAE

Cytokine ◽  
2001 ◽  
Vol 13 (1) ◽  
pp. 18-24 ◽  
Author(s):  
Kenji Murakami ◽  
Akihiko Uchiyama ◽  
Takehiro Kokuho ◽  
Yasuyuki Mori ◽  
Hiroshi Sentsui ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Interferon Γ ◽  
Biologically Active ◽  
Expression Systems ◽  
Silkworm Larvae ◽  
Baculovirus Gene

Obtaining recombinant capsid protein VP60 of rabbit haemorrhagic disease virus and its antigenic and immunogenic activity study

2020 ◽  
pp. 34-37
Author(s):  
Aleksey N. Mukhin ◽  
◽  
Konstantin P. Alekseev ◽  
Anna S. Moskvina ◽  
Oleg A. Verkhovskiy ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Virus ◽  
Expression System ◽  
Hemorrhagic Disease ◽  
Gene Expression System ◽  
Rabbit Hemorrhagic Disease ◽  
Baculovirus Gene ◽  
The Russian Federation ◽  
Haemorrhagic Disease ◽  
Capsid Protein Vp60

Viral hemorrhagic disease of rabbits is an acute, highly contagious disease characterized by the phenomena of hemorrhagic diathesis in all organs, especially in the lungs and liver. The causative agent of viral haemorrhagic disease of rabbits is a virus of haemorrhagic disease of rabbits, belonging to the family Caliciviridae, genus Lagovirus. Currently, there are 4 genogroups of lagoviruses, two pathogenic: GI1 (GI1a-GI1d) and GI2, and two non-pathogenic: GI3 and GI4. The greatest danger to rabbits in the Russian Federation is posed by viruses of the GI1 genotype. The virulence of these viruses for rabbits is extremely high, the incubation period is 48-72 hours. Clinically, the disease is almost not manifested. Mortality can reach 100%. For the prevention of HBV in the Russian Federation, inactivated tissue vaccines are used, which are a suspension of the liver of rabbits infected with virulent strains of the rabbit hemorrhagic disease virus. Currently, in veterinary practice, subunit recombinant vaccines based on virus proteins obtained in the baculovirus gene expression system are increasingly used. The authors obtained the recombinant VP60 virus of rabbit hemorrhagic disease of the GI1 genotype in the baculovirus gene expression system and studied its antigenic and immunogenic activity for rabbits. It was found that the recombinant capsid protein VP60 of the hemorrhagic disease virus, administered to rabbits at a dose of 50 pg, causes the synthesis of specific antibodies in animals, detected by enzyme immunoassay and in the hemagglutination inhibition reaction, and protects 80% of animals during control infection with the virulent strain «Voronezh-87» at a dose of 1000 LD 50. These data indicate the possibility of using this protein as a specific component of a subunit vaccine against rabbit hemorrhagic disease caused by strains of the GI1 gene group.

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