scholarly journals Identification and characterization of vlf-1, a baculovirus gene involved in very late gene expression.

1994 ◽  
Vol 68 (12) ◽  
pp. 7746-7756 ◽  
Author(s):  
J R McLachlin ◽  
L K Miller
Virology ◽  
1994 ◽  
Vol 200 (2) ◽  
pp. 260-269 ◽  
Author(s):  
Timothy D. Morris ◽  
Jason W. Todd ◽  
Bradford Fisher ◽  
Lois K. Miller

2002 ◽  
Vol 83 (8) ◽  
pp. 2015-2023 ◽  
Author(s):  
Asha Acharya ◽  
Karumathil P. Gopinathan

Late gene expression factors, LEF-4, LEF-8, LEF-9 and P47 constitute the primary components of the Autographa californica multinucleocapsid polyhedrovirus (AcMNPV)-encoded RNA polymerase, which initiates transcription from late and very late promoters. Here, characterization of lef-9 and lef-8, which encode their corresponding counterparts, from Bombyx mori NPV is reported. Transcription of lef-9 initiated at two independent sites: from a GCACT sequence located at −38 nt and a CTCTT sequence located at −50 nt, with respect to the +1 ATG of the open reading frame. The 3′ end of the transcript was mapped to a site 17 nt downstream of a canonical polyadenylation signal located 7 nt downstream of the first of the two tandem translational termination codons. Maximum synthesis of LEF-9 was seen from 36 h post-infection (p.i.). The transcription of lef-8 initiated early in infection from a GTGCAAT sequence that differed in the corresponding region from its AcMNPV counterpart (GCGCAGT), with consequent elimination of the consensus early transcription start site motif (underlined). Peak levels of lef-8 transcripts were attained by 24 h p.i. Immunocopurification analyses suggested that there was an association between LEF-8 and LEF-9 in vivo.


2013 ◽  
Vol 175 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Wei Yu ◽  
Chao-Yi Du ◽  
Yan-Ping Quan ◽  
Zuo-Ming Nie ◽  
Jian Chen ◽  
...  

2013 ◽  
Vol 87 (24) ◽  
pp. 13214-13225 ◽  
Author(s):  
A. Handisurya ◽  
P. M. Day ◽  
C. D. Thompson ◽  
C. B. Buck ◽  
Y.-Y. S. Pang ◽  
...  

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