scholarly journals Near-Infrared Luciferin Analogs for In Vivo Optical Imaging

2021 ◽  
Author(s):  
Ryohei Saito-Moriya ◽  
Rika Obata ◽  
Shojiro A. Maki
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
Bioluminescence Imaging ◽  
Imaging System ◽  
Biological Tissues ◽  
Nir Light ◽  
In Vivo Bioluminescence Imaging ◽  
Firefly Bioluminescence ◽  
In Vivo Optical Imaging

The firefly bioluminescence reaction has been exploited for in vivo optical imaging in life sciences. To develop highly sensitive bioluminescence imaging technology, many researchers have synthesized luciferin analogs and luciferase mutants. This chapter first discusses synthetic luciferin analogs and their structure–activity relationships at the luminescence wavelength of the firefly bioluminescence reaction. We then discuss the development of luciferin analogs that produce near-infrared (NIR) light. Since NIR light is highly permeable for biological tissues, NIR luciferin analogs might sensitively detect signals from deep biological tissues such as the brain and lungs. Finally, we introduce two NIR luciferin analogs (TokeOni and seMpai) and a newly developed bioluminescence imaging system (AkaBLI). TokeOni can detect single-cell signals in mouse tissue and luminescence signals from marmoset brain, whereas seMpai can detect breast cancer micro-metastasis. Both reagents are valid for in vivo bioluminescence imaging with high sensitivity.

scholarly journals Role of Fluorophore Charge on the In Vivo Optical Imaging Properties of Near-Infrared Cyanine Dye/Monoclonal Antibody Conjugates

2015 ◽  
Vol 27 (2) ◽  
pp. 404-413 ◽  
Author(s):  
Kazuhide Sato ◽  
Alexander P. Gorka ◽  
Tadanobu Nagaya ◽  
Megan S. Michie ◽  
Roger R. Nani ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Optical Imaging ◽  
Near Infrared ◽  
Cyanine Dye ◽  
Antibody Conjugates ◽  
Imaging Properties ◽  
In Vivo Optical Imaging

scholarly journals Bright and stable near-infrared Pluronic–silica nanoparticles as contrast agents for in vivo optical imaging

2016 ◽  
Vol 4 (33) ◽  
pp. 5560-5566 ◽  
Author(s):  
Lesan Yan ◽  
Huiquan Wang ◽  
Anqi Zhang ◽  
Calvin Zhao ◽  
Yongping Chen ◽  
...  
Keyword(s):  
Lymph Node ◽  
Sentinel Lymph Node ◽  
Contrast Agents ◽  
Optical Imaging ◽  
Silica Nanoparticles ◽  
Near Infrared ◽  
Circulation Time ◽  
In Vivo Optical Imaging

The IR780@NPs exhibited excellent characteristics for in vivo imaging with a long circulation time and high retention in tumor and sentinel lymph node.

scholarly journals Near-Infrared Fluorescent Nanoprobes for in Vivo Optical Imaging

Nanomaterials ◽  
2012 ◽  
Vol 2 (2) ◽  
pp. 92-112 ◽  
Author(s):  
Chai-Hoon Quek ◽  
Kam W. Leong
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
In Vivo Optical Imaging

scholarly journals Near-infrared-emissive polymersomes: Self-assembled soft matter for in vivo optical imaging

2005 ◽  
Vol 102 (8) ◽  
pp. 2922-2927 ◽  
Author(s):  
P. P. Ghoroghchian ◽  
P. R. Frail ◽  
K. Susumu ◽  
D. Blessington ◽  
A. K. Brannan ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Soft Matter ◽  
Near Infrared ◽  
Self Assembled ◽  
In Vivo Optical Imaging

Synthesis and evaluation of curcumin-based near-infrared fluorescent probes for the in vivo optical imaging of amyloid-β plaques

2021 ◽  
pp. 105167
Author(s):  
Yong Dae Park ◽  
Mayank Kinger ◽  
Changho Min ◽  
Sang Yeob Lee ◽  
Youngjoo Byun ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Amyloid Β ◽  
In Vivo Optical Imaging

scholarly journals Pharmacokinetics and Biodistribution of Human Serum Albumin-TIMP-2 Fusion Protein Using Near-Infrared Optical Imaging

2011 ◽  
Vol 14 (3) ◽  
pp. 368 ◽  
Author(s):  
Mi-Sook Lee ◽  
Young Han Kim ◽  
Yeon Joo Kim ◽  
Seung-Hae Kwon ◽  
Jeong-kyu Bang ◽  
...  
Keyword(s):  
Body Weight ◽  
Fusion Protein ◽  
Optical Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Umbilical Vein ◽  
Cancer Therapeutics ◽  
Fluorescence Measurement ◽  
Standard Curve

Purpose TIMP-2 has been studied as an attractive cancer therapeutic candidate, and a TIMP-2 fusion protein (HSA/TIMP-2) displayed effective anticancer activity, despite a lack of information about its pharmacokinetics (PK) and biodistribution. The purpose of this work was to assess the PK and biodistribution of HSA/TIMP-2 as well as to quantify accumulated HSA/TIMP-2 in tumors. Methods Cy5.5 near-infrared (NIR) fluorescence was conjugated to the HSA/TIMP-2 protein (Cy5.5–HSA/TIMP-2) for monitoring spatio-temporal changes in vivo. For PK and biodistribution analysis, 0.2 μg/g body weight of Cy5.5–HSA/TIMP-2 was injected into MAT-LyLu prostate tumor xenografts, which were then imaged using an IVIS-200 optical imaging system. To quantify the accumulated HSA/TIMP-2 in tumors, we introduced a standard curve with depth-corrected fluorescence measurement. Results In the vascular tube formation assay with human umbilical vein endothelial cells (HUVECs), Cy5.5–HSA/TIMP-2 showed an antiangiogenic effect. In prostate cancer xenografts, Cy5.5–HSA/TIMP-2 exhibited a prolongation of blood half-life to 19.6 h and relatively preferential distribution to the tumor. The amount of tumor-accumulated Cy5.5–HSA/TIMP-2 was calculated to be 4.5 ± 0.5 ng/g body weight at 2 days, representing 2.25 ± 0.25% of the initial dose. Conclusions We evaluated the pharmacokinetic profile and biodistribution of HSA/TIMP-2 with favorable results, providing new information for more effective approaches to cancer therapeutics using HSA/TIMP-2. Additionally, real-time in vivo fluorescence imaging analysis using a depth-corrected standard curve may serve as a platform to quantify biodistributed drug in anticancer therapeutic studies. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Synthesis of chitosan-coated near-infrared layered double hydroxide nanoparticles for in vivo optical imaging

2012 ◽  
Vol 22 (12) ◽  
pp. 5503 ◽  
Author(s):  
Pei-Ru Wei ◽  
Shih-Hsun Cheng ◽  
Wei-Neng Liao ◽  
Kun-Che Kao ◽  
Ching-Feng Weng ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Layered Double Hydroxide ◽  
Near Infrared ◽  
Double Hydroxide ◽  
In Vivo Optical Imaging ◽  
Layered Double Hydroxide Nanoparticles

Near-infrared luminescent nanomaterials for in-vivo optical imaging

2008 ◽  
Vol 2 (1) ◽  
pp. 021920 ◽  
Author(s):  
Peter K. Bachmann
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
In Vivo Optical Imaging

scholarly journals In Vivo Optical Imaging of Acute Cell Death Using a Near-Infrared Fluorescent Zinc−Dipicolylamine Probe

2011 ◽  
Vol 8 (2) ◽  
pp. 583-590 ◽  
Author(s):  
Bryan A. Smith ◽  
Seth T. Gammon ◽  
Shuzhang Xiao ◽  
Wei Wang ◽  
Sarah Chapman ◽  
...  
Keyword(s):  
Cell Death ◽  
Optical Imaging ◽  
Near Infrared ◽  
In Vivo Optical Imaging
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