In vitro growth response of Artemisia annua seeds to different concentrations of plant growth regulators

2011 ◽  
Vol 10 (77) ◽  
Author(s):  
Jamaleddine Z. O.
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Growth Response ◽  
Artemisia Annua ◽  
In Vitro Growth

scholarly journals In Vitro Growth and Regeneration of Drosera spatulata Labill. on Various Media

HortScience ◽  
1996 ◽  
Vol 31 (6) ◽  
pp. 1033-1034 ◽  
Author(s):  
Mirna Curkovic Perica ◽  
Jasna Berljak
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Sodium Hypochlorite ◽  
Growth Regulators ◽  
Shoot Tips ◽  
Ms Medium ◽  
In Vitro Growth ◽  
In Vitro Multiplication

Conditions for in vitro multiplication and flowering of Drosera spatulata plants were established. Shoot tips of greenhouse-grown plants were sterilized with 1% or 0.5% sodium hypochlorite. The influence of different media concentrations, hormone supplementation, and pH was investigated. Full MS medium without growth regulators was the best for regeneration and multiplication of plants. Regenerated shoots rooted spontaneously on medium without growth regulators and without transfer to additional medium. In 3 months, 100 to 200 plants were generated per explant. Flowering was induced on media supplemented with plant growth regulators. Plants were acclimatized on sterile peat.

scholarly journals Effects of different plant growth regulators on in vitro growth of Aloe vera L.

2020 ◽  
Vol 49 (1) ◽  
pp. 159-162
Author(s):  
Unaiza Wahab ◽  
Muhammad Ashfaq ◽  
Muhammad Sajjad ◽  
Shabnum Shaheen ◽  
Riffat Sadique ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Aloe Vera ◽  
Leaf Explants ◽  
Leaf Node ◽  
Ms Medium ◽  
In Vitro Growth

An attempt was made to standardize the appropriate concentration of different growth regulators for successful in vitro growth of different explants (leaf, node and internode) of Aloe vera L. Results demonstrated best in vitro growth in leaf explants in MS medium supplemented with BAP (1.0 mg/l) and NAA (1.0 mg/l) at 26 ± 2ºC) with pH 5.70 using agar solidified medium and 16 hrs photoperiod.

scholarly journals Effect of analogues of plant growth regulators on in vitro growth of eukaryotic plant pathogens

2004 ◽  
Vol 53 (1) ◽  
pp. 58-64 ◽  
Author(s):  
V. Flors ◽  
M. C. Miralles ◽  
E. Varas ◽  
P. Company ◽  
C. Gonzalez-Bosch ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Pathogens ◽  
In Vitro Growth

Impact of precursors and plant growth regulators on in vitro growth, bioactive lignans, and antioxidant content of Phyllanthus species

2016 ◽  
Vol 52 (6) ◽  
pp. 598-607 ◽  
Author(s):  
Annamalai Muthusamy ◽  
Huliyar Narasimhamurthy Nagendra Prasad ◽  
Erathodi Ramachandran Sanjay ◽  
Mattu Radhakrishna Rao ◽  
Kapaettu Satyamoorthy
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Growth ◽  
Antioxidant Content

Artemisinin Production by Shoot Regeneration of Artemisia annua L. Using Thidiazuron

2008 ◽  
Vol 63 (1-2) ◽  
pp. 96-100 ◽  
Author(s):  
Wanwimon Lualon ◽  
Wanchai De-Eknamkul ◽  
Hiroyuki Tanaka ◽  
Yukihiro Shoyama ◽  
Waraporn Putalun
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Artemisia Annua ◽  
Multiple Shoot ◽  
Stem Explants ◽  
Ms Medium ◽  
Artemisia Annua L ◽  
Bud Induction

An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem explants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The sources of explants as well as plant growth regulators in the medium were found to influence the multiple shoot induction. The result shows that the stem segment cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg/l thidiazuron (TDZ) gave a perfect shoot formation (100%) and good shoot multiplication (57 shoots/explant) after 2 weeks of culture. Healthy regenerated shoots were elongated and rooted in MS medium without hormones. The artemisinin content in plants regenerated from stem explants using 0.1 mg/l TDZ was (3.36 ± 0.36) μg/mg dry weight and two-fold higher than that of in vitro grown plants of the same age [(1.73 ± 0.23) μg/mg DW]. This system exhibited a potential for a rapid propagation of shoots from the stem explant and makes it possible to develop a clonal propagation of A. annua.

scholarly journals IMPACT OF GROWTH REGULATORS ON IN VITRO GROWTH OF BANANA (MUSA SPP) CULTURED: A REVIEW

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Katkam Priyanka
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Multiplication Rate ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Musa Spp ◽  
Micro Propagation

Banana is important fruit crop in horticulture where it propagates through suckers which takes immense time for production and with low multiplication rate, to overcome these type of situation some protocols have been made such as the micro propagation where the tissue culture of banana is applied with help of plant growth regulators such as auxins and cytokinins are used at different concentration to attain the definite good results till now many studies had been done in the tissue culture of banana. In the present study it was observed that the explants cultured in MS medium containing 4 mg/l BAP + 0.5 mg/l IAA had highest number of shoot buds and number of shoots. Similar result was studied by Muhammad et al. (2007) where the highest multiplication ratio was observed at 4 mg/l BAP along with 1 mg/l IAA. Habiba et al. (2002) and Ahmed et al. (2014) reported that 4 mg/l BAP in combination with 2 mg/l IAA shown remarkable results.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

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