Optimization of explants surface sterilization condition for field grown peach (Prunus persica L. Batsch. Cv. Garnem) intended for in vitro culture

The main aim of present study was to overcome the problems associated with the in vitro culture initiation in Terminalia arjuna. The micropropagation of tree species is not easy as shrubs and herbs. Many problems encountered from explant collection to in vitro culture establishment. The problems that have been occurred during T. arjuna micropropagation were culture contamination, phenolic exudation, bud growth inhibition, shoots yellowing and leaf fall. All these problems have been solved by applying certain treatments prior to explant collection and inoculation. The mother tree was lopped in November months (six months prior to explant collection) to remove any inhibitory substance and release bud growth. Different sterilizing agents were used to minimize the bacterial and fungal contamination. Some modification in culture media (use of different concentration of NH4NO3 and KNO3 salts and adenine sulphate) was done. Surface sterilization of nodal explants collected from lopped branches with 0.1% HgCl2 for 8 min., treatment with chilled antioxidant solution (Ascorbic acid, Citric acid and PVP) and half strength of NH4NO3 and KNO3 salts of MS medium supported 100% bud break response with proliferation of green and healthy in vitro shoots. Removing these hurdles already in the initial stage of micropropagation is very important and maximize mass in vitro propagation of this medicinally important Arjun tree.

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How Carbon Source and Seedcoat Influence the In Vitro Culture of Peach (Prunus persica L. Batsch) Immature Seeds

HortScience ◽

10.21273/hortsci15502-20 ◽

2021 ◽

pp. 1-2

Author(s):

Margarita Pérez-Jiménez ◽

Alfonso Guevara-Gázquez ◽

Antonio Carrillo-Navarro ◽

José Cos-Terrer

Keyword(s):

Seed Germination ◽

Carbon Source ◽

In Vitro Culture ◽

Seedling Growth ◽

Woody Plant ◽

Prunus Persica ◽

In Vitro Germination ◽

Immature Seeds ◽

Woody Plant Medium

The effects of carbon source and concentration and of seedcoat were tested on the in vitro germination of peach seeds derived from crosses performed in the field. Seeds were extracted from the fruit and cultured in Woody Plant Medium (WPM) supplemented with sucrose, glucose, or sorbitol at concentrations of 15, 30, and 45 g·L−1. The percentage of germination as well as the root and hypocotyl lengths were measured after the stratification process and before acclimatization. Seedcoat did not have any influence on seed germination in any tested media and genotype. Glucose at a concentration of 15 g·L−1 and sucrose at 15, 30, and 45 g·L−1 resulted in greater stem seedling growth. The root developed the most when seeds were cultured in media with 15 or 30 g·L−1 of sucrose.

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SEED AND SEEDLING SURFACE-STERILIZATION FOR IN VITRO CULTURE OF TILLANDSIA GARDNERI (BROMELIACEAE)

Acta Horticulturae ◽

10.17660/actahortic.2012.961.50 ◽

2012 ◽

pp. 383-389

Author(s):

A.C.R. Pinto ◽

M.E.S.P. Demattê ◽

S. Creste ◽

J.C. Barbosa

Keyword(s):

In Vitro Culture ◽

Surface Sterilization

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Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Jahangirnagar University Journal of Biological Sciences ◽

10.3329/jujbs.v5i2.32514 ◽

2017 ◽

Vol 5 (2) ◽

pp. 15-26 ◽

Cited By ~ 1

Author(s):

Raihan I Raju ◽

Shyamal K Roy

Keyword(s):

In Vitro Culture ◽

Basal Medium ◽

Nodal Segments ◽

Garden Soil ◽

Root Induction ◽

Ms Medium ◽

Mass Propagation ◽

Surface Sterilization ◽

Half Strength

Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoogs (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.Jahangirnagar University J. Biol. Sci. 5(2): 15-26, 2016 (December)

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Rhizome Buds Disinfection for Preparation of Red Ginger (Zingiber officinale Roxb. var. rubrum Rosc.) In Vitro Culture

Media Pharmaceutica Indonesiana (MPI) ◽

10.24123/mpi.v3i1.2836 ◽

2020 ◽

Vol 3 (1) ◽

pp. 19-26

Author(s):

Popy Hartatie Hardjo ◽

Alfian Hendra Krisnawan

Keyword(s):

In Vitro Culture ◽

Axenic Culture ◽

Zingiber Officinale ◽

Culture Initiation ◽

Surface Sterilization ◽

Sterilization Method ◽

Ginger Rhizome ◽

Red Ginger ◽

Rhizome Buds

The success of culture initiation depends on explant surface sterilization techniques. Suitable concentration, combinations, and duration of exposure of sterilizing agents are important to raise in vitro culture successfully. The aim of this work is to obtain the suitable sterilization method for explant buds of red ginger rhizome to get the axenic culture. Four sterilizing agents, fungicide, bactericide, Cefotaxime antibiotic, and NaOCl were tested for sterilization by various concentration and duration of exposure. The results showed that sterilizing agents 200 mg/L Cefotaxime and 100 mg/L Benomyl combined with NaOCl decreased the contamination of explants, and achieved 20% axenic culture.

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