scholarly journals Nuclear genome size and karyotype analysis in Papaver for BAC library construction

2005 ◽  
Vol 21 (2) ◽  
pp. 145-150
Author(s):  
T. K. Kyrylenko ◽  
O. I. Martynenko ◽  
O. G. Alkhimova
Keyword(s):  
Genome Size ◽  
Karyotype Analysis ◽  
Bac Library ◽  
Nuclear Genome ◽  
Library Construction

Nuclear genome size estimation and karyotype analysis of Lycium species (Solanaceae)

2013 ◽  
Vol 151 ◽  
pp. 46-50 ◽  
Author(s):  
Jianjun Chen ◽  
Xiaoli Liu ◽  
Linyao Zhu ◽  
Ying Wang
Keyword(s):  
Genome Size ◽  
Karyotype Analysis ◽  
Nuclear Genome ◽  
Size Estimation

Nuclear genome size and karyotype analysis ofAgave angustifoliaHaw. “Cimarron” and “Lineño” (Asparagales, Asparagaceae)

Caryologia ◽  
2017 ◽  
Vol 70 (2) ◽  
pp. 93-101
Author(s):  
G. Palomino ◽  
J. Martínez ◽  
P. Romero ◽  
R. Barba-González ◽  
B. Rodríguez-Garay
Keyword(s):  
Genome Size ◽  
Karyotype Analysis ◽  
Nuclear Genome

scholarly journals Nuclear genome size and karyotype analysis in Mammillaria species (Cactaceae)

Caryologia ◽  
2006 ◽  
Vol 59 (2) ◽  
pp. 177-186 ◽  
Author(s):  
Del Angel Christian ◽  
Guadalupe Palomino ◽  
Armando García ◽  
Ignacio Méndez
Keyword(s):  
Genome Size ◽  
Karyotype Analysis ◽  
Nuclear Genome

scholarly journals Electrophoretic Karyotypes and Genome Sizing of the Pathogenic Fungus Paracoccidioides brasiliensis

1998 ◽  
Vol 36 (3) ◽  
pp. 742-747 ◽  
Author(s):  
Maria Isabel Nogueira Cano ◽  
Patrícia Silva Cisalpino ◽  
Ivan Galindo ◽  
José Luiz Ramírez ◽  
Renato Arruda Mortara ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Confocal Microscopy ◽  
Genome Size ◽  
Chromosomal Location ◽  
Karyotype Analysis ◽  
Paracoccidioides Brasiliensis ◽  
Pathogenic Fungus ◽  
Nuclear Genome ◽  
Chromosomal Band ◽  
Chromosomal Bands

Here we present the karyotype analysis and genome sizing ofParacoccidioides brasiliensis, a pathogen refractory to conventional genetic analysis. We have established pulsed-field gel electrophoresis (PFGE) conditions to resolve the high-molecular-weight chromosomal bands of two clinical isolates ofP. brasiliensis. Both isolates showed four megabase-sized bands, ranging from 2.0 to 10.0 Mbp. Significant differences in chromosome sizes and in the chromosomal location of genes for the gp43 antigen and chitin synthase were found. Different technical approaches were employed to estimate the DNA content and to define the ploidy ofP. brasiliensis. An estimated genome size in the range of 45.7 to 60.9 Mbp was provided by the analysis of data generated by measuring the amplitude of fluorescence intensity of DAPI (4′,6-diamidino-2-phenylindole)-stained nuclei (by confocal microscopy). The nuclear genome size estimated by confocal microscopy is twice that estimated by the average sum of the molecular weight of chromosome-sized DNA molecules by PFGE, suggesting that each separatedP. brasiliensis chromosomal band is diploid.

scholarly journals Origin of the Eukaryotic Cell

2017 ◽  
Vol 01 (02) ◽  
pp. 108-120 ◽  
Author(s):  
Nick Lane
Keyword(s):  
Protein Synthesis ◽  
Host Cell ◽  
Genome Size ◽  
Energy Savings ◽  
Nuclear Genome ◽  
Eukaryotic Cell ◽  
Eukaryotic Cells ◽  
Bacterial Genomes ◽  
Complex Cells ◽  
Life On Earth

All complex life on Earth is composed of ‘eukaryotic’ cells. Eukaryotes arose just once in 4 billion years, via an endosymbiosis — bacteria entered a simple host cell, evolving into mitochondria, the ‘powerhouses’ of complex cells. Mitochondria lost most of their genes, retaining only those needed for respiration, giving eukaryotes ‘multi-bacterial’ power without the costs of maintaining thousands of complete bacterial genomes. These energy savings supported a substantial expansion in nuclear genome size, and far more protein synthesis from each gene.

Nuclear genome size variation in fleshy-fruited Neotropical Myrtaceae

2008 ◽  
Vol 276 (3-4) ◽  
pp. 209-217 ◽  
Author(s):  
Itayguara Ribeiro da Costa ◽  
Marcelo Carnier Dornelas ◽  
Eliana Regina Forni-Martins
Keyword(s):  
Genome Size ◽  
Size Variation ◽  
Nuclear Genome ◽  
Genome Size Variation
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