Effect of Relative Humidity in Air on the Transmission of Respiratory Viruses

Viral respiratory infections have plagued mankind over its known history. Unfortunately, there has been a lack of meaningful progress in preventing the spread of viral respiratory infections globally. The central dogma appears to be that viruses are the villains. This framing focuses on a viral load balance (VLB) in the air. It follows that physical dilution through various means have been the primary focus of attempts to reduce the spread of infections. The problem of obesity provides a good example of how paradigm blindness can slow down progress in a field. Obesity has been framed as an energy balance disorder that blames overeating and lack of exercise for weight gain. Reframing obesity as a disorder of fat metabolism and storage caused by the quantity and quality of carbohydrates in the diet, referred to as the carbohydrate-insulin model (CIM), opened an alternative line of questioning with a testable hypothesis. Similarly, we postulate an alternative way to frame the spread of viral respiratory infections that would lead to new insights and potentially new ways to prevent infections. It has long been recognized that viral respiratory infections show a pronounced seasonal variation, referred to as seasonal forging, such that they increase in the winter but decrease or virtually disappear in the summer. In temperate regions, people spend over 90% of their time indoors. This is, therefore, where most respiratory infections are expected to occur. Evidence has been accumulating for decades on the strong correlation between variations in indoor relative humidity (RH) and variations in infection rates. Within a RH Goldilocks zone of 40%-60%, encapsulated viruses like influenza and SARS are optimally inactivated outside the infected host. Below 40% and above 80%, viruses can survive for extended periods in the air or on surfaces. This may explain in part the seasonality of infections as the indoor level of RH in winter is typically about 20% and above 40% in summer in temperate regions. However, the mechanism for the inactivation at midrange RH (in summer) is not well understood. This paper offers a hypothesis that could explain these observations. We have demonstrated that H2O2 and other reactive oxygen species (ROS) are formed spontaneously at the water-air interface of pure water microdroplets. Using only water and a nebulizing gas in the presence of oxygen, we have demonstrated the significant disinfectant potential of pure water microdroplets caused by the activity of H2O2 and other ROS. We postulate that spontaneous H2O2 and ROS formation in viruses containing exhaled microdroplets have a similar virucidal effect at mid-range RH. The droplet evaporation rate is sufficient to concentrate the solutes and provide enough time for reactions to occur at significantly higher rates than in bulk solutions. The concentration of H2O2 has also been shown to be positively correlated to RH. In addition, several other ROS/RNS may be present or formed through interactions with H2O2 that may act as even more effective virucide disinfectants to inactivate the virus. Below RH 40% evaporation happens too rapidly for these reactions to make an impact before the droplet is desiccated, and above RH 80% the solutes remain too diluted. Rapid inactivation of viruses at midrange RH may therefore play a greater role in preventing infections than physical dilution of virus load in the air through excessive mechanical ventilation. Similar to obesity, we suggest that a new paradigm that considers virus infectivity outside the host rather than the virus load balance in the air alone could greatly contribute to our understanding of respiratory infections. The proposed new “Relative Humidity Infectivity” RHI paradigm could explain the causal mechanisms underlying seasonal respiratory infections. This can point to better prevention strategies that avoid further distortion of our indoor environment and create conditions within which humans can thrive and be optimally protected. We need more focus on testing the various hypotheses and more data to determine which of the two paradigms will lead us in the right direction or how to use the best of both in an optimal combination. The stakes cannot be higher, and the potential for eradicating future viral respiratory pandemics with nature-based solutions may be right under our noses, literally.

Inhaled Water and Salt Suppress Respiratory Droplet Generation and COVID-19 Incidence and Death on US Coastlines

Dry air alters salt and water balance in the upper airways and increases the risks of COVID-19 among other respiratory diseases. We explored whether such upper airway variations in salt and water balance might alter respiratory droplet generation and potentially contribute to observed impacts of airway hydration on respiratory disease. In a randomized 4-arm study of 21 healthy human subjects we found that the breathing of humid air, the wearing of cotton masks, and the delivery of (sodium, calcium, and magnesium chloride) salt droplets sized to deposit in the nose, trachea, and main bronchi similarly reduce the exhalation of respiratory droplets by approximately 50% ([Formula: see text] ¡ 0.05) within 10 minutes following hydration. Respiratory droplet generation returns to relatively high baseline levels within 60–90 minutes on return to dry air in all cases other than on exposure to divalent (calcium and magnesium) salts, where suppression continues for 4–5 hours. We also found via a preliminary ecological regression analysis of COVID-19 cases in the United States between January 2020 and March 2021 that exposure to elevated airborne salt on (Gulf and Pacific) US coastlines appears to suppress by approximately 25%–30% ([Formula: see text] ¡ 0.05) COVID-19 incidence and deaths per capita relative to inland counties — accounting for ten potential confounding environmental, physiological, and behavioral variables including humidity. We conclude that the hydration of the upper airways by exposure to humidity, the wearing of masks, or the breathing of airborne salts that deposit in the upper airways diminish respiratory droplet generation and may reduce the risks of COVID-19 incidence and symptoms.

Envisioning the “Air Economy” — Powered by Reticular Chemistry and Sunlight for Clean Air, Clean Energy, and Clean Water

Addressing the three major stresses facing our planet, clean air, clean energy, and clean water, is within our reach. At present, new materials such as metal-organic frameworks and covalent organic frameworks, produced by reticular chemistry, are at the forefront of efforts to capture carbon dioxide from air and harvest water from air. We envision that the products of these two capture processes (carbon dioxide and water) can be fed into a conversion cycle in which they are used to produce fuels and chemicals via artificial photosynthesis. The use of air as a nonpolluting, cyclable, and sustainable resource for carbon and water can be powered by sunlight. We describe how the scientific basis for realizing this vision is either already achieved or being established, and that in the fullness of time this paradigm may lead to new global industries and a thriving “air economy.”

Authorization of Vaccines in the European Union

The current COVID-19 pandemic has had a vertiginous accelerating effect on the time from identification of a new virus to a vaccine being deployed. A key step in this journey, the marketing authorization process, is under intense scrutiny and time pressure. The marketing authorization is a necessary gateway that protects public health and provides healthcare professionals and the general public with reliable and relevant evidence reflected in the product information to allow the safe and effective use of these vaccines. Regulators have the expertise and the duty to evaluate vaccines’ benefits and risks and, importantly, monitor safety during clinical use. This evaluation is more important than ever during a pandemic—and must be allowed to be as thorough as ever.

Path to Success and Future Impact of Nucleic Acid Vaccines: DNA and mRNA

The rapid development of mRNA vaccines for COVID-19 has both astonished the world and raised concerns about their safety, perhaps because many people do not realize the decades’ long efforts for nucleic acid vaccines, both mRNA and DNA vaccines, including the licensure of several veterinary DNA vaccines. This manuscript traces the milestones for nucleic acid vaccine research and development (R&D), with a focus on the immune and safety issues they both raised and answered. The characteristics of the two entities are compared, demonstrating the similarities and differences between them, the advantages and disadvantages, which might lead toward using one or the other technology for different indications. In addition, as the SARS-CoV-2 pandemic has once again highlighted the importance of One Health, that is, the interactions between animal and human pathogens, focus will also be given to how DNA vaccine utilization and studies both in large domestic animals and in wildlife pave the way for more integrated approaches for vaccines to respond quickly to, and prevent, the global impacts of emerging diseases.

The Mole, Avogadro’s Number and Albert Einstein

The mole a concept and Avogadro’s number are discussed as sought by Albert Einstein in his PhD thesis of 1905. Einstein would probably have regarded the metric system of units based on centimetre-gram-second (cgs) preferable to today’s SI system and specifically he would have rejected a recent SI suggestion to redefine Avogadro’s constant as based on a nonatomistic continuum description of matter. He would probably also have preferred keeping a dualistic definition of mole able of bookkeeping both mass and number of particles: we advocate that here and call it the ‘Einstein Definition’ and as Avogadro’s number we shall adopt an integer, the cube of 84446888 as suggested by Fox and Hill, providing also a definition of the kilogram based on the atomic mass of the carbon 12 isotope. Einstein was the first to explain the microscopic movements of pollen grains reported by Robert Brown in 1828 and his explanation that the particles move as a result of an unequal number of water molecules bumping into them from opposite sides was what finally made the scientific world accept the atom theory in its modern shape. In a cosmic diffusion analogy, pollen or bacterial spores moving randomly in outer space driven by the solar winds between solar systems can be envisaged. Applying Einstein’s diffusion theory, one can argue that life might have emerged from far outside of our planet from billions of solar systems, though not from outside of our Milky Way galaxy. As a curiosity we note that the number of solar systems (stars) in the Universe has been estimated to be of the order of Avogadro’s number.

Airway Hygiene in Children and Adults for Lowering Respiratory Droplet Exposure in Clean and Dirty Air

Respiratory illness threatens the learning potential of hundreds of millions of children around the world. We find in a human volunteer study involving three sites and 253 volunteers that respiratory droplets — of the size and nature to potentially contain COVID-19, influenza, allergens and other contaminants — diminish in number on exhalation by up to 99% via the “airway hygiene” administration of a nasal saline rich in calcium. Exhaled particles were significantly higher and efficacy of airway hygiene greatest at the site (Bangalore India) with highest fine particle ambient air burden. We argue for the use of airway hygiene for pandemic and post-pandemic global learning.

How Technology Has Thrown Us Out of Balance with Nature and Why We Need to Get Back In

We have created a technology-based environment in an effort to control our world, but we need to understand that the biological forces that shaped us continue to play a role in our future. As more and more species go extinct because of human activity, we must learn how to reconnect with nature to fit in with the earth’s rhythm, not try to conquer it.

From Poliomyelitis to the COVID-19 Pandemic. Reflections by a Virologist

Never before have the media focused on a single infectious disease as they have in the case of the coronavirus COVID-19 pandemic that started to spread globally from China at the end of 2019. The consequences of the pandemic on health, economics, and the societal conditions of isolated individuals have been discussed from a range of different perspectives. Virologists are expected to be capable of providing immediate answers to many different kinds of questions—how and under what conditions is an individual infectious, what are the relative roles of the different arms of the immune system, do reinfections occur, when will a vaccine preventing infection with the virus become available, what are the possibilities of developing antiviral drugs capable of interfering with the disease, and so on. In many cases there are no immediate answers, since virologists globally are still in the middle of researching the particular problem in the focus of interest. The only proper answer to demanding questions of this kind should be “Welcome to the workshop of virologists.” However, what needs to be emphasized is that the tools available to understand the details of the interaction of a particular virus and the various organs in an infected human host have changed dramatically during the somewhat more than a hundred years of studies of viruses.