scholarly journals Possible replacement of cytokinins and auxins in culture in vitro

2019 ◽  
Vol 25 ◽  
pp. 293-297
Author(s):  
T. M. Oliynik ◽  
R. B. Kovbasenko ◽  
A. P. Dmitriev ◽  
P. G. Dulniev
Keyword(s):  
Cell Culture ◽  
Callus Tissue ◽  
Ms Medium ◽  
Pyridine Derivatives ◽  
Culture In Vitro ◽  
Somaclonal Variability ◽  
Proposed Modification ◽  
New Generation ◽  
Derivatives Of

Aim. To perform an analysis of the effectiveness of our proposed modification of the classical MS medium in which auxin and cytokinin are replaced by derivatives of the class of tetrahydrothio-fendioxide and pyridine. Methods. In this study, the work with cell culture in vitro, in particular aseptic sprouting of seeds, microclonal reproduction, callusogenesis and initiation of various types of morphogenesis was carried out according to known techniques. Two varieties of potatoes (Slovyanka, Lugovskaya) and two varieties of tomato (Lagidny, Bobrytsky) were used to induce somaclonal variability. Results. The ability of preparations of tetrahydrothiophenedioxide and pyridine derivatives to perform the function of auxins and cytokinins in the MS medium in the callusogenesis of Solanacea cultures in vitro was established. Conclusions. It has been shown that with the use of the new generation drug (tetrahydrothiophenedioxide-pyridine), as a substitute for auxin and cytokinin in the MS medium, the growth of callus tissue is even increased. The production of the pilot batch of this substitute proved to be much cheaper than synthesis or the purchase of phytohormones. Keywords: modification of MS medium, phytohormones substitute, tetrahydro-thiophenedioxide-pyridine, potato, tomato.

scholarly journals Development of a New Fluid Shearing System for Cell Culture in Vitro

2019 ◽  
Vol 626 ◽  
pp. 012013
Author(s):  
Pengfei Wu ◽  
Xin Wang ◽  
Lilan Gao ◽  
Chunqiu Zhang
Keyword(s):  
Cell Culture ◽  
Culture In Vitro

Mouse Periosteal Cell Culture, in vitro Differentiation, and in vivo Transplantation in Tibial Fractures

BIO-PROTOCOL ◽  
2021 ◽  
Vol 11 (15) ◽  
Author(s):  
Simon Perrin ◽  
Anais Julien ◽  
Oriane Duchamp de Lageneste ◽  
Rana Abou-Khalil ◽  
Céline Colnot
Keyword(s):  
Cell Culture ◽  
Tibial Fractures ◽  
In Vitro Differentiation ◽  
Culture In Vitro ◽  
Periosteal Cell

scholarly journals Comparative analyses of stevioside between fresh leaves and in-vitro derived callus tissue from Stevia rebaudiana Bert. using HPLC

2015 ◽  
Vol 49 (4) ◽  
pp. 199-204 ◽  
Author(s):  
S Mahmud ◽  
S Akter ◽  
IA Jahan ◽  
S Khan ◽  
A Khaleque ◽  
...  
Keyword(s):  
Retention Time ◽  
Callus Tissue ◽  
Callus Formation ◽  
Leaf Explants ◽  
Stevia Rebaudiana ◽  
Poor Performance ◽  
The Other ◽  
Ms Medium ◽  
Green Callus

A protocol was developed to produce large amount of callus in short a period of time from leaf explants of Stevia rebaudiana Bert. The highest amount of white callus was obtained on MS medium supplemented with 2.5 mg/l 2, 4-D and 0.5 mg/l BAP after 3 weeks of inoculating leaf segments. On the other hand, 0.5 mg/l BAP and 1.0 mg/l Kn exhibits poor performance towards callus formation while after using 1.0 mg/l Kn alone did not develop any callus. In this experiment, highest amount of green callus was obtained when MS medium supplemented with 2.5 mg/l NAA and 10% coconut water was used. An improved analytical method HPLC was applied to analyze stevioside extracted from the leaf and callus of Stevia rebaudiana. The stevioside in each sample were analyzed by comparing their retention times with those of the standards. The retention time (RT) of stevioside for leaves were found 14.96 and for callus 13.81 mins. The percentage of stevioside content from leaves and callus was 12.19% and 12.62% respectively DOI: http://dx.doi.org/10.3329/bjsir.v49i4.22621 Bangladesh J. Sci. Ind. Res. 49(4), 199-204, 2014

Regulation of hormonal secretion and DNA synthesis in lactotrophs of the rat adenohypophysis in primary cell culture in vitro

1988 ◽  
Vol 105 (4) ◽  
pp. 580-582
Author(s):  
I. S. Komolov ◽  
V. P. Fedotov ◽  
D. Rappay ◽  
I. Fazekas ◽  
V. V. Abramova ◽  
...  
Keyword(s):  
Cell Culture ◽  
Dna Synthesis ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
Culture In Vitro ◽  
Hormonal Secretion

scholarly journals The Effect of Pyridinecarbothioamides on Isoflavonoid Production in Genista tinctoria Cultures in Vitro

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800
Author(s):  
Lenka Tůmová ◽  
Věra Klimešová ◽  
Anna Vildová
Keyword(s):  
Suspension Culture ◽  
Callus Culture ◽  
Culture Medium ◽  
Culture In Vitro ◽  
Elicitor Treatment ◽  
Pyrazinecarboxylic Acid ◽  
Genista Tinctoria ◽  
Derivatives Of ◽  
Maximum Content

Previous studies showed that derivatives of pyrazinecarboxylic acid used as elicitors increased either flavonoid or flavonolignan production. The effect of 2-(3-bromobenzylsulfanyl) pyridine-4-carbothioamide on isoflavonoids production in Genista tinctoria culture in vitro was tested. The highest isoflavonoid content (0.3 mg.g−1 DW) in callus culture was found after elicitor application at a concentration of 2.96 × 10−5 mol.L−1 after 24 h of treatment. The maximum daidzein production (0.2 mg.g−1 DW) was detected after 48 h of elicitor treatment at a concentration of 2.96 × 10−3 mol.L-1 and after 24 h of elicitor treatment at a concentration of 2.96 × 10−5 mol.L−1. Suspension culture produced the highest amount of isoflavonoids (0.3 mg.g−1 DW) after elicitor application at concentrations of 2.96 × 10−4 mol.L−1 after 24 and 168 h of treatment, and 2.96 × 10−5 mol.L−1 after 168 h of treatment. The highest content of genistin (0.3 mg. g−1 DW) was detected after 24 h of elicitor treatment at a concentration of 2.96 × 10−4 mol.L−1. The maximum content of daidzein (3.80 mg.L−1) released into the suspension culture medium was found after elicitor application at a concentration of 2.96 × 10−4 mol.L−1 after 12 h of treatment.

Sign in / Sign up

Export Citation Format

Share Document