scholarly journals The Effect of Pyridinecarbothioamides on Isoflavonoid Production in Genista tinctoria Cultures in Vitro

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800
Author(s):  
Lenka Tůmová ◽  
Věra Klimešová ◽  
Anna Vildová
Keyword(s):  
Suspension Culture ◽  
Callus Culture ◽  
Culture Medium ◽  
Culture In Vitro ◽  
Elicitor Treatment ◽  
Pyrazinecarboxylic Acid ◽  
Genista Tinctoria ◽  
Derivatives Of ◽  
Maximum Content

Previous studies showed that derivatives of pyrazinecarboxylic acid used as elicitors increased either flavonoid or flavonolignan production. The effect of 2-(3-bromobenzylsulfanyl) pyridine-4-carbothioamide on isoflavonoids production in Genista tinctoria culture in vitro was tested. The highest isoflavonoid content (0.3 mg.g−1 DW) in callus culture was found after elicitor application at a concentration of 2.96 × 10−5 mol.L−1 after 24 h of treatment. The maximum daidzein production (0.2 mg.g−1 DW) was detected after 48 h of elicitor treatment at a concentration of 2.96 × 10−3 mol.L-1 and after 24 h of elicitor treatment at a concentration of 2.96 × 10−5 mol.L−1. Suspension culture produced the highest amount of isoflavonoids (0.3 mg.g−1 DW) after elicitor application at concentrations of 2.96 × 10−4 mol.L−1 after 24 and 168 h of treatment, and 2.96 × 10−5 mol.L−1 after 168 h of treatment. The highest content of genistin (0.3 mg. g−1 DW) was detected after 24 h of elicitor treatment at a concentration of 2.96 × 10−4 mol.L−1. The maximum content of daidzein (3.80 mg.L−1) released into the suspension culture medium was found after elicitor application at a concentration of 2.96 × 10−4 mol.L−1 after 12 h of treatment.

In vitro stable regeneration of onion and garlic from suspension culture and chromosomal instability in solid callus culture

2005 ◽  
Vol 104 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Madhumita J. Mukhopadhyay ◽  
Papiya Sengupta ◽  
Sandip Mukhopadhyay ◽  
Sumitra Sen
Keyword(s):  
Suspension Culture ◽  
Callus Culture ◽  
Chromosomal Instability

scholarly journals Androgenesis of Red Cabbage in Isolated Microspore Culture In Vitro

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1950
Author(s):  
Anna Mineykina ◽  
Ludmila Bondareva ◽  
Alexey Soldatenko ◽  
Elena Domblides
Keyword(s):  
Culture Medium ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Antioxidant Properties ◽  
F1 Hybrids ◽  
Anthocyanin Content ◽  
Isolated Microspore Culture ◽  
Culture In Vitro ◽  
Red Cabbage

Red cabbage belongs to the economically important group of vegetable crops of the Brassicaceae family. A unique feature of this vegetable crop that distinguishes it from other members of the family is its unique biochemical composition characterized by high anthocyanin content, which gives it antioxidant properties. The production mainly uses F1 hybrids, which require constant parental lines, requiring 6–7 generations of inbreeding. Culture of isolated microspores in vitro is currently one of the promising methods for the accelerated production of pure lines with 100% homozygosity. The aim of this study is to investigate the factors and select optimal parameters for successful induction of red cabbage embryogenesis in isolated microspore culture in vitro and subsequent regeneration of DH plants. As a result of research, for the first time, it was possible to carry out the full cycle of obtaining DH plants of red cabbage from the induction of embryogenesis to their inclusion in the breeding process. The size of buds containing predominantly microspores at the late vacuolated stage and pollen at the early bi-cellular stage has to be selected individually for each genotype, because the embryoid yield will be determined by the interaction of these two factors. In the six samples studied, the maximum embryoid yield was obtained from buds 4.1–4.4 mm and 4.5–5.0 mm long, depending on the genotype. Cultivation of microspores was carried out on liquid NLN culture medium with 13% sucrose. The maximum number of embryoids (173.5 ± 7.5 pcs./Petri dish) was obtained on culture medium with pH 5.8 and heat shock at 32 °C for 48 h. Successful embryoid development and plant regeneration by direct germination from shoot apical meristem were achieved on MS culture medium with 2% sucrose and 0.7% agar, supplemented with 6-benzylaminopurine at a concentration of 1 mg/L. Analysis of the obtained regenerated plants, which successfully passed the stage of adaptation to ex vitro conditions by flow cytometry, showed that most of them were doubled haploids (up to 90.9%). A low number of seeds produced by self-fertilization in DH plants was observed.

Regeneration of plants from gynogenetic carrot calluses

2015 ◽  
Vol 2 (3) ◽  
pp. 49-54
Author(s):  
T. Vitsenia ◽  
O. Sergiyenko
Keyword(s):  
Culture Medium ◽  
Average Weight ◽  
Callus Line ◽  
Culture In Vitro ◽  
Regenerant Plants

Aim. To optimize the regeneration and adaptation stages of the methods of obtaining dihaploid carrot plants via gynogenesis in the culture in vitro, namely embryogenesis from gynogenetic callus, and to obtain normally developed regenerant plants and regenerant roots. Methods. Common biotechnological methods were applied. The gynogenetic carrot callus, line 345, was obtained from non-fertilized ovaries using the method, invented by the authors. Results. The culture medium, in which the yield of embryos from one gynogenetic callus in- creases 4-fold, and the yield of normal plants – 12-fold, was invented. While growing roots using regenerant plants in unprotected soil the mulching of soil with white agrofi ber ensures the survival of regenerants at the level of 86.5 % which is 16 % higher than the control (without mulching) and the increase in the average weight of roots up to 92.8 g (38.5 g more compared to the control). Conclusions. The regenerating and adaptive stages of obtaining dihaploid regenerant plants from gynogenetic carrot calluses, induced from non-fertilized ovaries in culture in vitro were optimized. The effi cient culture medium for embryogenesis from gynogenetic calluses was elaborated. It was established that soil mulching using white agrofi ber increases the survival of gynoge- netic plants in unprotected soil considerably and ensures the increase in the average weight of roots. It allows refusing from greenhouses or tunnels while growing regenerant roots of gynogenetic origin.

scholarly journals Features of development of hametophytes Botrychium multifidum (S.G. Gmel.) Rupr. culture in vitro

2020 ◽  
Vol 24 ◽  
pp. 00043
Author(s):  
Igor Krinitsyn ◽  
Dmitry Zontikov ◽  
Svetlana Zontikova ◽  
A. Baghizadeh ◽  
P. Behroozi ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Spore Germination ◽  
Culture Medium ◽  
Mineral Salts ◽  
Nutrient Media ◽  
Culture In Vitro ◽  
Initial Inoculum ◽  
Development Of Gametophytes ◽  
Ph Level

The work is devoted to studying the influence of the type of culture medium and pH on the development of gametophytes Botrychium multifidum in vitro. The spores obtained from sterilized sporangia were suspended in liquid nutrient media with initial inoculum of 10000 spores per 1 ml. Nutrient media tested in the study were composed of full Murashige and Skoog or Knudson mineral salts supplemented with kinetin (1 mg/l) and pH level 4.8-6.4. All stages of development, from spore germination to thallus and gametophyte formation, were observed in tissue culture. A low level of germinating spores was noted.

scholarly journals Effect of medium gelatinized component on the effectiveness of androgenesis in vitro Oryza sativa L.

2016 ◽  
Vol 14 (2) ◽  
pp. 178-180
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
D. V. Shpak ◽  
A. O. Dobrova ◽  
S. O. Ignatova
Keyword(s):  
Anther Culture ◽  
Culture Medium ◽  
Oryza Sativa L ◽  
Modified Starch ◽  
Culture In Vitro ◽  
Chemically Modified ◽  
Androgenesis In Vitro ◽  
Negative Effect ◽  
Callus Regeneration

Aims. To study the effect of chemically modified starch D–5aM in the culture medium on the efficiency of androgenesis in vitro in anther culture of rice. Methods. Obtaining of rice double haploid lines by anther culture in vitro. The statistical methods. Results The influence different variants of gellatyne source in culture medium on the processes of induction and regeneration in anther culture of rice were studied. The 119 green plants-regenerants were received. Conclusions. The negative effect on the formation of green regenerants using a gel-forming components of the chemically modified starch D–5aM was shown. Keywords: rice, anther culture in vitro, callus, regeneration, chemically modified starch.

scholarly journals Determination of Salt Tolerance of Oil Palm SUP-PSU 1 Using Cell Suspension Culture

2021 ◽  
Author(s):  
Thirasak Sukdee ◽  
Sompong Te-chato ◽  
SUREERAT YENCHON
Keyword(s):  
Electrical Conductivity ◽  
Growth Rate ◽  
Suspension Culture ◽  
Oil Palm ◽  
Culture Medium ◽  
Proline Content ◽  
Repeated Application ◽  
Fresh Weight

Abstract Oil palm is an oil crop that produces the highest oil yield throughout the year. Repeated application of fertilizer in oil palm orchard results in severe salinity of planting areas. Therefore, the objective of this study was to determine growth, physiochemistry and re-growth of calli as effected by sodium chloride (NaCl). The calli were cultured in liquid oil palm culture medium (OPCM) supplemented with different concentrations of NaCl (0, 50, 100, 150, 200, 300 and 400 mM), 0.1 mg/l dicamba and 200 mg/l ascorbic acid for 4, 8 and 12 weeks. The results showed that increase in concentrations of NaCl and exposure times decreased growth rate of cells in suspension culture. The concentration of NaCl which inhibited growth rate at 50 % (IC50) was 166.73 mM after culture for 12 weeks. Increase in concentrations of NaCl and exposure times increased electrolyte leakage (EL), changing of electrical conductivity (EC) medium and proline content. NaCl at concentration of 400 mM gave the highest EL, changing of EC medium and proline content at 82.22 %, 3.77 mS/cm and 64.93 µg/gFW, respectively. Over 200 mM NaCl oil palm cells cannot survive. After transfer treated callus clumps to OPCM without NaCl for 8 weeks, the clumps treated in 50 mM NaCl gave the highest fresh weight at 416.5 mg while 100 mM gave the highest average number of nodules per tube at 84 nodules. This result suggests that cells of oil palm in vitro could not stand to NaCl at concentration higher than 200 mM.

scholarly journals Synthesis and Antiviral Evaluation of SATE-Foscarnet Prodrugs and New Foscarnet—AZT Conjugates

1998 ◽  
Vol 9 (1) ◽  
pp. 41-52 ◽  
Author(s):  
C Meier ◽  
A-M Aubertin ◽  
M de Monte ◽  
A Faraj ◽  
J-P Sommadossi ◽  
...  
Keyword(s):  
Culture Medium ◽  
Chromatographic Purification ◽  
Alkyl Esters ◽  
Chemical Hydrolysis ◽  
B Virus ◽  
Immunodeficiency Virus ◽  
Hydrolysis Of ◽  
Derivatives Of ◽  
Anti Hiv

The synthesis of a range of di- and triester derivatives of phosphonoformate (PFA; foscarnet) as potential lipophilic, membrane-soluble prodrugs is described. In addition to normal alkyl esters in the carboxylate and phosphonate residues of PFA, the bioreversible S-(pivaloyl)thioethyl ( t-butyl-SATE) group was introduced in an attempt to deliver PFA after bioactivation inside the cells. Furthermore, PFA—AZT conjugates were prepared in order to develop combinational drugs. The key synthetic step was in all cases the formation of the P—C bond to build up the different PFA esters. In contrast to the diester derivatives, the triesters of PFA showed high hydrolytic instability during chromatographic purification. The compounds were evaluated in vitro for their ability to inhibit viruses in several tissue culture systems. All PFA alkyl di- and triesters proved poorly active or inactive against human immunodeficiency virus (HIV) and inactive against hepatitis B virus. In contrast, the PFA—AZT conjugates exhibited significant anti-HIV activity. However, this activity was nearly completely lost in thymidine kinase-deficient cells, suggesting a fast unselective chemical hydrolysis of the conjugates to yield the nucleoside analogue AZT in the cell culture medium. Furthermore, no synergistic effect of PFA and AZT was observed.

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