scholarly journals Effect of 1-naphthylacetic and indol-3-acetics acid on the intensity of callusogenesis and organogenesis of Linum usitatissimum L. in vitro

2021 ◽  
Vol 28 ◽  
pp. 100-105
Author(s):  
S. V. Mishchenko

Aim. Investigate the effect of auxins of exogenous origin in nutrient medium in vitro on the germination and organogenesis intensity in Linum usitatissimum L. convar. elongatum (‘Hlinum’ variety) at the constant concentration of 6-benzylaminopurine (BAP). Methods. Hypocotyl segments were cultured on Murashige and Skoog nutrient medium supplemented with sucrose (30 g/l) and phytohormones at various concentrations. Other conditions: photoperiod 16 hours, relative humidity 60–80%, air temperature 22–24°C. Results. Common flax has a great capacity to form callus and shoots under the effect of the following factors: 1) only auxins, 2) only cytokines, 3) combinations of auxins and cytokines. Somatic embryogenesis is also possible on a nonhormonal nutrient medium. Conclusions. For somatic embryogenesis in vitro, the optimal concentrations of BAP can be expressed as 1.0 ≤ BAP ≤ 1.75, the optimal concentrations of BAP for the medium supplemented with 1-naphthylacetic (NAA, 0.05 mg/l) 0.5 ≤ BAP ≤ 2.0, the optimal concentration of NAA for the medium supplemented with BAP (1.0 mg/l) 0.025 ≤ NAA ≤ 0.150, and the optimal concentrations of indol-3-acetics acid (IAA) for the medium supplemented with BAP (1.0 mg/l) 0.05 ≤ IAA ≤ 0.50. Keywords: Linum usitatissimum L., in vitro, phytohormones, callus, organogenesis.

2010 ◽  
Vol 32 (3) ◽  
pp. 527-533 ◽  
Author(s):  
Iva Smykalova ◽  
Miroslava Vrbova ◽  
Eva Tejklova ◽  
Martina Vetrovcova ◽  
Miroslav Griga

2020 ◽  
Vol 11 ◽  
Author(s):  
Shankhamala Bose ◽  
Thibaut Munsch ◽  
Arnaud Lanoue ◽  
Laurine Garros ◽  
Duangjai Tungmunnithum ◽  
...  

Author(s):  
S.V. Mishchenko ◽  
L.M. Krivosheeva

Goal. The study of different genetic origin collection samples of flax (Linum usitatissimum L.) for the frequency and intensity of callusogenesis and organogenesis in vitro conditions, the establishment of differences in convar. elongatum, intermedia and humile was the goal of our research. Results and Discussion. The L. usitatissimum L. species is significantly capable of forming callus and shoot regeneration in vitro under cultivation conditions with a photoperiod of 16 hours, relative humidity of 60 – 80 %, air temperature of 22 – 24°С and agarized Murashige and Skoog medium, supplemented 0.05 mg/L of 1‑naphthylacetic acid, 1.0 mg/L of 6‑benzylaminopurine and 30 g/L of sucrose. The frequency and intensity of callusogenesis and organogenesis depends on the genotype. Minimum and maximum values of signs were set for Глінум (UF0401603), Кром (UF0401494), Орион (UF0401867), Есмань (UF0402071), Белита (UF0402134), Nor Man (UF0401792), Mapun M.A. (UF0401819), Lisa (UF0401830), Taragvi (UF0401864), Visamo (1–356)/L. monnseo (UF0402178), Ручеек (UF0401897), Lirina (UF0401900), Опус (UF0402142), СКі‑1 (UF0402143), Ruta (UF0402228) samples. The frequency of callusogenesis was 15.0 – 100 %, the calus mass from one explant was 0.56 – 1.51 g, the frequency of organogenesis was 10.0 – 93.8 %, the number of shoots was 1.4 – 4.0 pieces and the height of the shoots was 0.78 – 2.37 cm. Conclusions. Collection samples of Глінум (Ukraine), Кром (Russia), Visamo (1‑356)/L. monnseo (Czech Republic), Ручеек (Russia) and СКі‑1 (USA) were the best of complex signs (callusogenesis frequency, organogenesis frequency and number of shoots). Elongata flax and humile flax are characterized by the highest frequency of callusogenesis and organogenesis on hypocotyl and epicotyl explants, intermedia flax forms the largest mass of callus from the explant, the number of regenerated shoots and their height, intermedia flax has the greatest variation of the studied signs.


Sign in / Sign up

Export Citation Format

Share Document