scholarly journals An in vitro screening method for antitumor and/or antitumorigenic substances involving the transformation of chick embryo fibroblasts infected with Rous sarcoma virus.

1984 ◽  
Vol 37 (3) ◽  
pp. 235-238 ◽  
Author(s):  
MIEKO TAKEUCHI ◽  
YOSHIO SATO ◽  
KAZUO NITTA
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Screening Method ◽  
In Vitro Screening ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts

Long-term cultures of chick embryo fibroblasts transformed by the Schmidt–Ruppin strain (D) of Rous sarcoma virus

1976 ◽  
Vol 22 (10) ◽  
pp. 1474-1479
Author(s):  
Lorraine Leblond-Larouche ◽  
Réjean Morais
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Rous Sarcoma ◽  
Viable Cells ◽  
Deficient Medium ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts ◽  
Roller Cultures

Attempts have been made to keep in vitro, for extended periods of time, cultures of chick embryo fibroblasts transformed by the Schmidt–Ruppin strain of Rous sarcoma virus, subgroup D. Roller cultures of transformed chick cells kept in serum-deficient medium can be maintained without subcultivation for up to 6 months. The confluent cultures continuously release viruses and viable tumor cells into the medium. The released cells can be plated and have characteristics of growth and morphology which are relatively stable with time until the culture degenerates. Cells released at later stages of the culture produced substantially more viruses than those released earlier, suggesting that cell selection or differentiation occurs during long-term cultivation in low serum concentration. Long-term cultures of untransformed chick embryo fibroblasts can also be maintained in the same way. The release of viable cells by these confluent cultures, however, is negligible.

scholarly journals Decreased levels of collagen mRNA in rous sarcoma virus-transformed chick embryo fibroblasts

1978 ◽  
Vol 253 (16) ◽  
pp. 5869-5874
Author(s):  
B.H. Howard ◽  
S.L. Adams ◽  
M.E. Sobel ◽  
I. Pastan ◽  
B. de Crombrugghe
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Collagen Mrna ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts

scholarly journals COMPARATIVE STUDIES IN ROUS SARCOMA WITH VIRUS, TUMOR CELLS, AND CHICK EMBRYO CELLS TRANSFORMED IN VITRO BY VIRUS

1962 ◽  
Vol 115 (1) ◽  
pp. 245-251 ◽  
Author(s):  
Robert M. Dougherty ◽  
Herbert R. Morgan
Keyword(s):  
Chick Embryo ◽  
Tumor Cells ◽  
Comparative Studies ◽  
Rous Sarcoma Virus ◽  
Rous Sarcoma ◽  
Transformed Fibroblasts ◽  
Embryo Cells ◽  
Sarcoma Virus

Chick embryo fibroblasts infected in vitro with Rous sarcoma virus have properties similar to tumor cells when injected into virus-immune chickens. When such virus-transformed fibroblasts are injected into normal chickens, they apparently participate in the production of tumors independent of their release of virus and are thus apparently malignant in vivo.

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