scholarly journals Peer Review #1 of "colocr: an R package for conducting co-localization analysis on fluorescence microscopy images (v0.1)"

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7255
Author(s):  
Mahmoud Ahmed ◽  
Trang Huyen Lai ◽  
Deok Ryong Kim

Background The co-localization analysis of fluorescence microscopy images is a widely used technique in biological research. It is often used to determine the co-distribution of two proteins inside the cell, suggesting that these two proteins could be functionally or physically associated. The limiting step in conducting microscopy image analysis in a graphical interface tool is the selection of the regions of interest for the co-localization of two proteins. Implementation This package provides a simple straightforward workflow for loading fluorescence images, choosing regions of interest and calculating co-localization measurements. Included in the package is a shiny app that can be invoked locally to interactively select the regions of interest where two proteins are co-localized. Availability colocr is available on the comprehensive R archive network, and the source code is available on GitHub under the GPL-3 license as part of the ROpenSci collection, https://github.com/ropensci/colocr.


2019 ◽  
Author(s):  
Mahmoud Ahmed ◽  
Trang Huyen Lai ◽  
Deok Ryong Kim

Background The co-localization analysis of fluorescence microscopy images is a widely used tech- nique in biological research. It is often used to determine the co-distribution of two proteins inside the cell, suggesting that these two proteins could be functionally or physically associated. The limiting step in conducting microscopy image analysis in a graphical interface tool is the selection of the regions of interest for the co-localization of two proteins. Implementation This package provides a simple straight forward workflow for loading fluorescence images, choosing regions of interest and calculating co-localization statistics. Included in the package is a shiny app that can be invoked locally to select the regions of interest where two proteins are interactively co-localized. Availability colocr is available on the comprehensive R archive network, and the source code is available on GitHub as part of the ROpenSci collection, https://github.com/ropensci/colocr. Keywords: R package, co-localization, image-analysis, fluorescence microscopy, statistics


2019 ◽  
Author(s):  
Mahmoud Ahmed ◽  
Trang Huyen Lai ◽  
Deok Ryong Kim

Background The co-localization analysis of fluorescence microscopy images is a widely used tech- nique in biological research. It is often used to determine the co-distribution of two proteins inside the cell, suggesting that these two proteins could be functionally or physically associated. The limiting step in conducting microscopy image analysis in a graphical interface tool is the selection of the regions of interest for the co-localization of two proteins. Implementation This package provides a simple straight forward workflow for loading fluorescence images, choosing regions of interest and calculating co-localization statistics. Included in the package is a shiny app that can be invoked locally to select the regions of interest where two proteins are interactively co-localized. Availability colocr is available on the comprehensive R archive network, and the source code is available on GitHub as part of the ROpenSci collection, https://github.com/ropensci/colocr. Keywords: R package, co-localization, image-analysis, fluorescence microscopy, statistics


Author(s):  
Saskia Delpretti ◽  
Florian Luisier ◽  
Sathish Ramani ◽  
Thierry Blu ◽  
Michael Unser

2021 ◽  
pp. 102168
Author(s):  
C. Ritter ◽  
T. Wollmann ◽  
J.-Y. Lee ◽  
A. Imle ◽  
B. Müller ◽  
...  

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66198 ◽  
Author(s):  
Jenna L. Mueller ◽  
Zachary T. Harmany ◽  
Jeffrey K. Mito ◽  
Stephanie A. Kennedy ◽  
Yongbaek Kim ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document