scholarly journals Peer Review #3 of "Up-regulation of cryptochrome 1 gene expression in cotton bollworm (Helicoverpa armigera) during migration over the Bohai Sea (v0.2)"

2019 ◽  
Author(s):  
Z Zou
Keyword(s):  
Gene Expression ◽  
Peer Review ◽  
Helicoverpa Armigera ◽  
Bohai Sea ◽  
Cotton Bollworm ◽  
The Bohai Sea ◽  
Helicoverpa Armígera ◽  
Cryptochrome 1

Silencing of cytochrome P450 CYP6B6 gene of cotton bollworm (Helicoverpa armigera) by RNAi

2013 ◽  
Vol 103 (5) ◽  
pp. 584-591 ◽  
Author(s):  
X. Zhang ◽  
X. Liu ◽  
J. Ma ◽  
J. Zhao
Keyword(s):  
Gene Expression ◽  
Helicoverpa Armigera ◽  
Target Gene ◽  
Insect Pests ◽  
Cotton Bollworm ◽  
Gene Expression Level ◽  
Expression Level ◽  
Rnai Pathway ◽  
Helicoverpa Armígera ◽  
Lepidopteran Pest

AbstractRNA interference (RNAi) induced through double-stranded RNA (dsRNA) has been used widely to study gene function in insects. In this paper we demonstrate the efficacy of RNAi in the cotton bollworm, Helicoverpa armigera. Using CYP6B6 as the target gene, which is expressed in the fat baby and midgut of the lepidopteran pest H. armigera, we constructed the vector which expressed dsRNA of CYP6B6. Northern blot analysis showed that dsRNA expressed in the Escherichia coli (HT115) was target gene. The results also showed that the gene expression level and protein expression level of H. armigera larvae fed with dsRNA expressed by E. coli were significantly lower than those of all controls, but the gene expression level was more obvious than that at the protein level; significant lethality differences were also found between HT115 bacteria containing L4440-dsC1 treatment and HT115 bacteria containing L4440 vector or CK (ddH2O) in instar larvae on 4 day when continuous feeding, 32.45% mortality was recorded in the group of feeding HT115 bacteria containing L4440-dsC1 on 10 day. Our results suggest that the RNAi pathway can be exploited to control insect pests.

scholarly journals Up-regulation of cryptochrome 1 gene expression in cotton bollworm (Helicoverpa armigera) during migration over the Bohai Sea

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8071
Author(s):  
Liyu Yang ◽  
Yingjie Liu ◽  
Philip Donkersley ◽  
Pengjun Xu
Keyword(s):  
Helicoverpa Armigera ◽  
Negative Selection ◽  
Bohai Sea ◽  
Purifying Selection ◽  
Migration Behavior ◽  
Negative Selection Pressure ◽  
Strong Negative Selection ◽  
Helicoverpa Armígera ◽  
Novel Transcripts ◽  
Cryptochrome 1

Cryptochromes (CRYs) are flavoproteins and play a pivotal role in circadian clocks which mediate behavior of organisms such as feeding, mating and migrating navigation. Herein, we identified novel transcripts in Helicoverpa armigera of six isoforms of cry1 and seven isoforms of cry2 by Sanger sequencing. Phylogenetic analysis showed that the transcripts of cry1 and cry2 align closely with other insect crys, indicating within-species divergence of Hacry. A dn/ds analysis revealed that the encoding sequence of the cry1 was under purifying selection by a strong negative selection pressure whereas the cry2 was less constraint and showed a less strong purification selection than cry1. In general, Hacrys were more abundantly transcribed in wild migrating populations than that in laboratory maintained populations, and expression of the cry2 was lower than cry1 in all samples tested. Moreover, when compared with the migrating parental population, offspring reared in laboratory conditions showed a significant reduction on transcription of the cry1 but not cry2. These results strongly suggest that cry1 was more related to the migration behavior of H. armigera than cry2.

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